Isku-darka aan-doog lahayn ee isku-darka
Faa'iidooyinka adeegga
●Falanqaynta wadajirka ah ee Mrna iyo LNcrna: Adoo isku daraya tirinta qoraalka sheekooyinka MRNA ee daraasadda Lancna iyo bartilmaameedyadooda, waxaa suurtagal ah in la helo dulmar guud oo ku saabsan hannaanka gacanta.
●Khibrad ballaaran: Kooxdayadu waxay u keentaa waayo-aragnimo badan oo ku saabsan mashruuc kasta, oo leh rikoor waddo ah oo ka badan 23,000 muunado ah oo ku saabsan noocyada tusaalaha ah ee BMK ee kaladuwan iyo mashaariicda Lancna.
●Xakamaynta tayada adag ee adag: Waxaan ka hirgelinaa qodobbada kontoroolka core ee dhammaan marxaladaha, tusaale ahaan muunadda iyo u diyaarinta maktabadda si ay u helaan isku xigxiga iyo bioinformatics. Korjoogteyntan sifiican loo kormeer ayaa hubiya gaarsiinta natiijooyin tayo sare leh oo joogto ah.
●Taageerada iibka ka dib: Balankeenna wuxuu ku fidsan yahay dhameystirka mashruuca ee leh muddo 3 bilood iibka iibka ah ka dib. Inta lagu jiro waqtigan, waxaan bixinaa dabagal dabagal mashruuc, gargaar dhibaatayn, iyo Q & SS & Kalfadhiyo si wax looga qabto wixii su'aalo ah ee la xiriira natiijooyinka.
Shuruudaha muunadda iyo dhalmada
| Maktabad | Boosteejo | Xogta lagu taliyay | XIGASHADA QC |
| RrNa dhammaatay Maktabadda Joogtada ah ee RRNA | Iftiimina PE150 | 10-16 GB | Q3t85% |
Nucleotatides:
| CODKA. (NG / μL) | Tirada (μg) | Daahir | Sharaf |
| 80 | ≥ 0.8 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Rin≥6.0; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
Dhirta:
Xidid, asliga ah ama petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
● Xayawaanka:
Wadnaha ama xiidmaha: 450 mg
Viscera ama maskaxda: 240 mg
Murqaha: 600 mg
Lafaha, timaha ama maqaarka: 1.5g
● Arthropods:
Cayayaanka: 9g
Crustacea: 450 mg
Dhiiga oo dhan:2 tuubooyin
● Unugyada: 106 unugyada
● Serum iyo plasma: 6 ml
Keenista muunad
Weelka: 2 ml centrifouge tuubada (foil tin tin laguma talinayo)
Calaamadaha muujinta: Kooxda + waxay ku daydaa tusaale ahaan a1, A2, A3; B1, B2, B3.
Shixnad:
1. Ice-ICE: Saamiyada ayaa loo baahan yahay in lagu buuxiyo bacaha oo lagu aasay baraf qalalan.
2. Tuunnada lagu qurxiyo
Socodka shaqada adeegga
Naqshadeynta Tijaabada
Keenista muunad
Soo saarida RNA
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Bioinformatics
Falanqaynta Gene ee Kala-duwan (DEEGs) Falanqaynta
Tirada muujinta LNCRIN - Isku-darka
Kobcinta hiddaha bartilmaameedka Lancra
Wadajirnimada Mrna iyo Lancna Point Falanqaynta
Baadh horumarka ay fududeysay adeegyo casaan ee BMKNE-ga LNCRA iyada oo loo marayo aruurinta daaqsinta daabacaadaha.
JI, H. et al. (2020) 'aqoonsi, saadaalin shaqeynaya, iyo furaha LNCRA-yada LNCRIN ee LNCRNAS CLEARNAS ee LNCRINA ee Beerka', warbixinnada sayniska ee 2020 10: 10 (1), pp. 1-14. DOI: 10.1038 / S41598-02020-57451-7.
Jia, Z. et al. (2021) Falanqaynta Transpritomic-ka Dhexgalka Dhexgalka ayaa muujisay hababka difaaca jirka ee Cyhv-3-u adkaysta Cabbirka Carp-3, xuduudaha ee loo yaqaan 'Chipers', oo ah 12, p. 687151. DOI: 10.3389 / Fimmu.2021.687151 / Bibtex.
Wang, xji et al. (2022) 'is-dhexgalka bulshada is dhexgalka bulshada ee ku saleysan ee ku saleysan Shabakadaha Lineogenous RNAGNASS ee Kansarka Sambas ee unugyada yar: astaamaha moliga ah iyo musharixiinta daroogada', xuruufta muraayadaha indhaha, 12, p. 904865. Do: 10.3389 / Fonc.2022.904865 / Bibtex.
Xiao, L. et al. (2020) '' Fikradda hidda-wadaha ee shabakada 'Colecnworm' oo hoos imanaya sawir-qaadista POSYNESSIS ee populus ', joornaalka bayootarka', 18 (4), pp. 1015-1026. doi: 10.1111 / PBI.13270.
Zheng, h. et al. (2022) Shabakad sharci oo caalami ah oo loogu talagalay muujinta hiddaha ee caagga ah iyo isu-duwaha dheef-shiid kiimikaad ee aan caadi ahayn ee unugyada cudurada ka yar ee cudurka '' Hashimototo 'ee cudurka qanjirada' Hashimoto's thyrialogy, 13, p. 879824. Do: 10.3389 / fimmu.2022.879824 / Bibtex.
