Alaabta

Isku-xiridda Hiddo-sidaha Dhan ee Dhirta/Xayawaanka

Dhirta/Xayawaanada Dhan ee Taxanaha Genome-ga Sawirka

Dib-u-soo-celinta genome-ga oo dhan, oo sidoo kale loo yaqaan WGS, waxay awood u siinaysaa muujinta isku-beddellada caadiga ah iyo kuwa naadirka ah ee dhammaan genome-ga oo ay ku jiraan Single Nucleotide Polymorphism (SNP), Tirtirka Gelinta (InDel), Kala duwanaanshaha qaab-dhismeedka (SV), iyo Kala duwanaanshaha Tirada Nuqulka (CNV) ).SV-yadu waxay ka kooban yihiin qayb weyn oo ka mid ah saldhigga kala duwanaanshiyaha marka loo eego SNPs waxayna saameyn weyn ku leeyihiin genome-ka, taas oo saameyn weyn ku leh noolaha.Dib u akhrinta dheer waxay u ogolaanaysaa in si sax ah loo aqoonsado jajabyo waaweyn iyo kala duwanaansho adag sababtoo ah akhrinta dheer waxay aad u fududaynaysaa ka gudubka koromosoomyada ee gobolada adag sida soo noqnoqoshada tandem, GC/AT-gobolada qani ku ah, iyo gobolada isbedbeddelka badan.

Platform: Illumina, PacBio, Nanopore

Nala soo xiriir

Faahfaahinta Adeegga

Natiijada Demo

Daabacaada sifada

1. Faa'iidooyinka Adeegga

Khibrad balaadhan oo ku saabsan isku xigxiga genome ee in ka badan 1000 nooc.

In ka badan 800 oo kiis oo la daabacay oo leh saameyn urursan oo ka badan 4000.

Falanqaynta bioinformatics oo dhamaystiran oo ku saabsan wacitaanka kala duwanaanshaha iyo falanqaynta shaqada.

2. Tilmaamaha Adeegga

Platform		Maktabadda	qoto dheer seq lagu taliyay
Illumina		PE 150	Loogu talagalay SNP, InDel wacitaanka ≥ 10x Wixii SV, CNY wac ≥ 30x
Nanopore		8 kb	Wixii SV, CNY wac ≥ 20x
Pacbio	CCS	15 kb	Wixii loogu talagalay SNP, InDel, SV, CNY wacitaanka ≥ 10x

3. Shuruudaha Tusaalaha

Platform		Conc.(ng/μL)	Qadarka (ng)	daahirnimo		Agaroose jel
				OD260/280	OD260/230	1. Nadiifi koox weyn oo aan lahayn ama xaddidnaynhoos u dhaca lagu arkay jel. 2. Maya ama xaddidan wasakheynta borotiinka RNA
Illumina		≥1	≥30	-	-
Nanopore		≥30	Waxay kuxirantahay soosaarka xogta 10μg / unug	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. Falanqaynta Xogta Noolaha

5. Socodka Shaqada Adeegga

Tusaalaha keenista

Soo saarista DNA

Dhismaha maktabadda

Isku xigxiga

Falanqaynta xogta

gaarsiinta xogta

Hore: BMKMANU S1000 Spatial Transcriptome

Xiga: Isku-dabaynta hal-xuduneed ee RNA

1) Tirakoobka Khariidaynta Genome

Shaxda 1aad ee natiijada khariidaynta

Jaantuska 1 Qaybinta cabbirka gelinta iyo akhrinta daboolka.

2) Kala duwanaansho ogaanshaha

Jaantuska 2 Tirakoobyada iyo sharraxaadda SNP/INDEL/SV ee ka mid ah Tusaalooyinka

Jaantuska 3 Qaybinta genome-ballaaran ee fasaxyada

3) Sharaxaada hawleed ee kala duwanaanshaha

2019	Isgaarsiinta dabeecadda	Isku soo wada duuboo-genome-ku wuxuu muujinayaa asalka Brassica napus iyo meesha hidda-socodka ee ku lug leh horumarintiisa
2020	PNAS	Asalkii koray iyo taariikhda qoysnimo ee kalluunka dahabka (Carassius auratus)
2021	Wargeyska Biotechnology ee dhirta	Tixraaca genome ee labada nooc ee jute la beeray
2021	Wargeyska Biotechnology ee dhirta	Saxeexa genomic ee khudradda iyo miraha saliidda ah allopolyploid Brassicajuncea iyo loci hidda-socodka oo xakameynaya ururinta glucosinolates
2019	Warshada Molecular	Isku soo wada duuboo genome-ga guud ee u ekaanshaha ururinta abuuritaanka kufsiga ee adduunka oo dhan ayaa daaha ka qaadaya aasaaska hidde-sidaha kala duwanaanshaha deegaankooda.
2022	Cilmi-baarista Dhirta	Falanqaynta ururrada guud ee genome waxay bixisaa aragtiyo molecular ee kala duwanaanshaha dabiiciga ah ee cabbirka iniinaha qaraha
2021	Wargeyska Tijaabada Dhirta	Daraasada ururka genome-ballaaran ayaa tilmaamaysa noocyada GhSAD1 ee siinaya dulqaad qabow suufka
2021	Wargeyska Tijaabada Dhirta	Daraasadda ururka guud ee genome iyo isbarbardhigga qoraalka qoraalka ayaa muujinaya QTL cusub iyo hiddo-wadaha murashaxiinta ee xakameynaya cabbirka tufaax ee abuurkii kufsiga.