Isku-xiridda Hiddo-sidaha Dhan ee Dhirta/Xayawaanka
Tilmaamaha Adeegga
● Diyaarinta maktabaddu waxay noqon kartaa mid caadi ah ama PCR-la'aan ah
● Waxaa laga heli karaa 4 goobood oo isku xiga: Illumina NovaSeq, MGI T7, Nanopore Promethion P48, ama PacBio Revio.
● Falanqaynta bioinformatic waxay diiradda saartay ogaanshaha noocyada kala duwan: SNP, InDel, SV iyo CNV
Faa'iidooyinka Adeegga
●Khibrad Balaadhan iyo Diiwaanada Daabacaadda: Khibrada la ururiyey ee isku xigxiga genome ee in ka badan 1000 nooc ayaa keentay in ka badan 1000 kiis oo la daabacay oo leh saameyn isugeyn ah oo ka badan 5000.
●Falanqaynta Bioinformatics oo dhamaystiran: Oo ay ku jiraan kala duwanaansho wacitaanka iyo sharraxaadda shaqada.
● Taageerada iibka kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
●Tibaaxo Dhamaystiran: Waxaan isticmaalnaa xog uruurin badan si aan si shaqeyn leh ugu sharaxno hiddo-wadaha kala duwanaansho la aqoonsaday oo aan sameyno falanqaynta kobcinta u dhigma, anagoo bixinayna fikrado ku saabsan mashruucyo cilmi baaris oo badan.
Tilmaamaha Adeegga
| Kala duwanaanshiyaha la aqoonsanayo | Istaraatiijiyada isku xigxiga | qoto dheer lagu taliyay |
| SNP iyo InDel | Illumina NovaSeq PE150 ama MGI T7 | 10x |
| SV iyo CNV (sax yar) | 30x | |
| SV iyo CNV (sax badan) | Nanopore Prom P48 | 20x |
| SNPs, Indels, SV iyo CNV | PacBio Revio | 10x |
Shuruudaha Tusaalaha
| Nudaha ama asiidhyada nucleic-ka ah ee la soo saaray | Illumina/MGI | Nanopore | PacBio
| ||
| Viscera xoolaha | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Murqaha Xoolaha | ≥ 5 g
| ≥ 5 g
| |||
| Dhiiga naasleyda | 1.5 ml | ≥ 0.5 ml
| ≥ 5 ml
| ||
| Dhiiga digaaga/Kalluunka | ≥ 0.1 ml
| ≥ 0.5 ml
| |||
| Dhirta- Caleen Cusub | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
| Unugyada Dhaqanka |
| ≥ 1x107
| ≥ 1x108
| ||
| Cayayaanka jilicsan / Shakhsi ahaaneed | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| DNA la soo saaray
| Xoog-saarista: ≥ 1 ng/µL Qadarka: ≥ 30 ng Xaddidan ama aan hoos u dhicin ama faddarayn
| Xoog-saarid Qadarka
OD260/280
OD260/230
Xaddidan ama aan hoos u dhicin ama faddarayn
| ≥ 40 ng/µL 4 µg / unug qulqulaya / muunad
1.7-2.2
≥1.5 | Xoog-saarid Qadarka
OD260/280
OD260/230
Xaddidan ama aan hoos u dhicin ama faddarayn | ≥ 50 ng/µL 10 µg / unug qulqulaya / muunad
1.7-2.2
1.8-2.5 |
| Diyaarinta Maktabada-La'aanta PCR: Isku-duubni≥ 40 ng/µL Qadarka ≥ 500 ng | |||||
Socodka Shaqada Adeegga
Tusaalaha keenista
Soo saarista DNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
gaarsiinta xogta
Waxa ku jira falanqaynta soo socota:
- Xakamaynta tayada xogta ceeriin
- Tirakoobyada toosinta genome-ka tixraaca
- Aqoonsiga kala duwan: SNP, InDel, SV iyo CNV
- Sharaxaada hawleed ee kala duwanaanshaha
Tirakoobyada isku toosan ee genome-ka tixraaca - qaybinta qoto dheer ee isku xigxiga
SNP oo wacaya muunado badan
Aqoonsiga InDel - tirakoobka dhererka InDel ee gobolka CDS iyo gobolka-ballaaran genome
Kala duwanaanshiyaha qaybinta genome-ga - Dhulka Circos
Tilmaanta shaqaynaysa ee hiddo-sidaha leh noocyo la aqoonsaday - Gene Ontology
Chai, Q. iyo al. (2023) 'Glutathione S-transferase GhTT19 ayaa go'aamisa midabaynta ubaxa ubaxa iyada oo loo marayo nidaaminta ururinta anthocyanin ee suufka', Journal Biotechnology Journal, 21(2), p. 433. doi: 10.1111/PBI.13965.
Cheng, H. iyo al. (2023) 'Heerka koromosoomyada duurjoogta Hevea brasiliensis genome wuxuu bixiyaa qalab cusub oo taranka lagu caawiyo genomic iyo loci qiimo leh si kor loogu qaado wax soo saarka caag', Plant Biotechnology Journal, 21(5), pp. 1058-1072. doi: 10.1111/PBI.14018.
Li, A. iyo al. (2021) 'Genome of the estuarine oyster' waxa uu bixiyaa aragtiyo ku saabsan saamaynta cimilada iyo balaastikada la qabsiga', Isgaadhsiinta Bayoolaji 2021 4:1, 4(1), bogga 1-12. doi: 10.1038/s42003-021-02823-6.
Zeng, T. iyo al. (2022) 'Falanqaynta isbeddellada genome iyo methylation ee digaagga asaliga ah ee Shiinaha waqti ka dib waxay siinaysaa aragtida ilaalinta noocyada', Bayoolojiga Isgaarsiinta, 5(1), bogga 1-12. doi: 10.1038/s42003-022-03907-7.
