Tshawb nrhiav cov noob thawj cov qauv ntawm qhov chaw qub ntawm ib qho muaj sia yog qhov tseem ceeb rau kev nkag siab cov hom thiab cov haujlwm ntawm nws cov hlwb. Txawm li cas los xij, kev soj ntsuam spatial transcriptome kev txwv tsis muaj kev txwv xws li kev tawm tsam qis lossis tsis txaus. Lub BMKManu S1000 Spatial nti, tsim los ntawm BMKManu, tuaj yeem pom hauv-situ cov cuab yeej cov ntaub ntawv ntawm subcellular daws teeb meem, ua kom muaj nuce kev txhais ntawm cov qauv nqaij.
Npaj txhij los ua peb cov khoom tshiab tshaj plaws! Ua kev zoo siab nws tso tawm, peb tab tom muab tshwj xeebYuav plaub tau txais ib qho pub dawbKev nce qib rau lub sijhawm tsawg xwb.
Cheebtsam
BMKManu S1000 Spatial nti thiab txuam cov khoom siv reagent suav nrog:
1) Cov Khoom Siv Ua Kom Zoo Dua Ntaub Ntawv
2) Cov khoom siv cov khoom siv tawm, muaj cov tshuaj tiv thaiv rau ntes MRNA hauv cov ntaub so ntswg rau kev npaj tom qab thiab npaj ua ntu zus.
3) Cov Khoom Siv Pib Pib: suav nrog lub thermostatic adapter thiab transcriavav sib nqus separator.
Cov hauv paus ntsiab lus
Lub nti yog raws li micropores thiab microbores thiab mRA tuaj yeem ntes tau hauv situ los ntawm oligo ntawm microbeads. Tus oligo muaj li plaub ntu :2, spatial barcode, umi, thiab poly (dt) vn. Tom qab cov ntaub so ntswg tau txuas rau nti, mRAMA raug tso tawm ntawm cov ntaub so ntswg los ntawm ib tog neeg ua kom enzyme. Txij li feem ntau MRNA 3 'xaus muaj poly-tus Tsov tus tw, lawv tau ntes los ntawm oligo nrog poly (dt) vn. Tom qab RT-PC thiab CDNA amplification, cov tsev qiv ntawv npaj thiab ua ntu zus, txoj haujlwm sib luag yog tiav los ntawm kev sib thooj. Qhov no tso cai rau Gene kev tshaj tawm kev tsom xam nyob rau cov chaw sib txawv thiab cov ntaub so ntswg heterogeneity.
Cov khoom lag luam zoo
Cov Ntawv Thov
Nws tuaj yeem siv rau feem ntau cov cheeb tsam ntawm feem ntau cov chaw ntawm kev tshawb fawb roj ntsha thiab kev kho mob, kev txhim kho biology, kev txhim kho biology, kev txhim kho kev tawm tsam ntawm cov kab ke nrog kev daws teeb meem.
Mob qog thiab kab mob:
Spatial heterogeneity thiab microenvironment ntawm hlav hlav thiab kab mob
OVSET thiab kev loj hlob ntawm cov qog thiab cov kab mob
Kev kho cov lus teb thiab kab mob
Kev loj hlob biology
SpatioCendEmail Atlas ntawm cov kabmob
Gene kev cai tswj cov txheej txheem thaum lub sijhawm kev txhim kho
● Kev txhawj xeeb txog kev txhawj xeeb
Bioti Kev Nyuaj Siab Cov Lus Teb
Abiotic Kev Nyuaj Siab Teb
● Kev txhaj tshuaj
Kev tiv thaiv kab mob hauv cov khoom nruab nrog
Kev tiv thaiv kev tiv thaiv kev qog thiab cov kab mob
Pathogenesis ntawm autoimmune kab mob
Tshuaj Xyuas Kev Tshuaj Xyuas Tshuaj Tshuaj
Kev siv tshuab ntawm cov yeeb tshuaj tiv thaiv
Kev tshawb nrhiav thiab kev txhim kho cov tshuaj tshiab
Cov Ntaub Ntawv thiab Demo Cov Ntaub Ntawv
BMKManu S1000 tau ua tiav txoj kev ua tau zoo nyob rau ntau pua tus mob hla cov nqaij sib txawv.
