Ukuphonononga iipateni zokuchazwa kofuzo kwindawo yokuqala yezinto eziphilayo kubalulekile ekuqondeni iindidi kunye nemisebenzi yeeseli zayo.Nangona kunjalo, iindlela zangoku zohlalutyo lwe-spatial transcriptome zinemida efana ne-output ephantsi okanye isisombululo esingonelanga.I-BMKMANU S1000 Spatial Chip, ephuhliswe yi-BMKMANU, inokubona i-in-situ ingcaciso yegenesis kumacandelo apheleleyo e-tissue kwisisombululo se-subcellular, eyenza ukutolika okungafaniyo kwesakhiwo sezicubu.
Zilungiselele ukufumana imveliso yethu entsha!Ukubhiyozela ukukhululwa kwayo, sinikezela ngokhethekileyoThenga ezine Fumana enye Mahalaukunyuswa kwexesha elithile kuphela.
Amacandelo eMveliso
I-BMKMANU S1000 itshiphu yendawo kunye nekiti ehambelanayo ye-reagent ibandakanya:
I-1) I-Tissue Optimization Kit, equlethe i-reagents yokuphucula izicubu (ixesha elifanelekileyo lokuhlola i-tissue permeabilization).
2) I-Gene Expression Kit, equlethe ii-reagents zokubamba i-mRNA kwizilayi zethishu ukulungiselela ukulungiswa kwethala leencwadi elilandelayo kunye nolandelelwano.
3) Ikiti yokuQalisa: Ibandakanya i-adapter ye-thermostatic kunye ne-transcriptome magnetic separator.
Umgaqo wobugcisa
I-chip isekelwe kwi-micropores kunye ne-microbeads, kwaye i-mRNA inokubanjwa kwi-situ nge-Oligo kwii-microbeads.I-Oligo yenziwe ngamacandelo amane: Read1, Spatial Barcode, UMI, kunye nePoly(dT)VN.Emva kokuba i-tissue ifakwe kwi-chip, i-mRNA ikhutshwa kwi-tissue nge-enzyme ye-permeabilization.Ekubeni uninzi lweziphelo ze-mRNA 3' zinePoly-A umsila, zibanjwa yi-Oligo ngePoly(dT)VN.Emva kwe-RT-PCR kunye nokwandiswa kwe-cDNA, ukulungiswa kwethala leencwadi kunye nolandelelwano, ukulandelwa kwendawo kufezekiswa ngeBhakhowudi yeSithuba.Oku kuvumela uhlalutyo lokubonakaliswa kwemfuza kwiindawo zendawo kunye ne-tissue heterogeneity.
Iinzuzo zeMveliso
Usetyenziso
Ingasetyenziswa kwiindawo ezininzi zebhayoloji kunye nophando lwezonyango, kubandakanya ithumba, isifo, i-immunology, kunye nebhayoloji yophuhliso, inceda ukufezekisa impumelelo emitsha kwezi nkalo ngohlalutyo lwembonakalo yendawo kwisisombululo esincinci.
● Ithumba kunye nezifo:
I-Spatial heterogeneity kunye ne-microenvironment yamathumba kunye nezifo
Ukuqala kunye nokuphuhliswa kwamathumba kunye nezifo
Impendulo yonyango lwamathumba kunye nezifo
●Ibhayoloji yoPhuhliso
Iatlasi yeSpatiotemporal yamalungu
Iindlela zokulawula imfuza ngexesha lophuhliso
●Ukusabela Ngoxinezeleko
Impendulo yoxinzelelo lwe-Biotic
Impendulo yoxinzelelo lweAbiotic
●I-Immunology
Ukusabela kwamajoni omzimba kwi-organ transplantation
Iinkqubo zokuzivikela ezifweni kunye nezifo
I-Pathogenesis yezifo ezizimele
● Uhlalutyo lokunganyangeki kweziyobisi
Iinkqubo zokunganyangeki ngamachiza
Uphando nophuhliso lwamachiza amatsha
Amatyala kunye neDatha yeDemo
I-BMKMANU S1000 iye yaqinisekiswa ukusebenza kumakhulu eemeko kwiintlobo ezahlukeneyo zeethishu.
