Qiyaasta qaaska ah ee goynta ah (slaf-seq)
Alwaax alwaax ah
Nidaamka Farsamo
Astaamaha adeegga
● Isku xigxiga lovasegaq oo leh PE150.
● Maktabadda diyaarinta oo leh laba geesood, karti u yeelo barkadaha ka badan 1000 shay.
● Farsamadahan waxaa loo isticmaali karaa ama iyada oo aan la helin tixraac loogu talagalay, oo leh dhuumaha saldhiga ah ee loo yaqaan 'bioinffilies' oo loo yaqaan 'Belinf' kiis kasta:
Iyada oo la tixraacayo tixraaca Genome: SNP iyo daahfurka Indel
Iyada oo aan la tixraacin Genome: Muunad isku-dhafan oo muunad ah iyo daahfurka SNP
● InsilicoMarxaladda naqshadeynta ee hordhaca ah ayaa la baarayaa si loo helo kuwa dhalinaya qaybinta labiska ee calaamadaha Slaf ee wadada dhinaceeda.
● Intii lagu gudajiro tijaabinta hore, saddex shay oo enzyme ah ayaa lagu tijaabiyaa 3 saac oo lagu soo saaro 9 maktabado SLAF, waxaana macluumaadkan loo adeegsadaa si loo doorto isku darka enzyme-ka ugu habboon ee mashruuca.
Faa'iidooyinka adeegga
●Baabuur-gallada hiddaha ee sare: Isku-daridda Nidaamka Codka-Sare ee Sareynta Laba-labo-labo-labo ayaa u oggolaanaya taxanaha isku mar ee dadka waaweyn, iyo cod-weyneynta gaarka ah waxay kor u qaadeysaa hufnaanta, hubinta in nambarada sumadda ay buuxiyaan shuruudaha kaladuwan ee su'aalaha cilmi-baarista ee kaladuwan.
● Ku tiirsanaanta hoose ee hiddo-wadaha: Waxaa lagu dabaqi karaa noocyada leh oo aan tixraac lahayn oo aan tixraac lahayn.
●Naqshadeynta qorsheynta dabacsan: Hal-enzyme, laba-enzyme, dheefshiidka badan, iyo noocyo kala duwan oo enzymes ah ayaa dhammaantood loo xulan karaa si ay ugu daboolaan yoolalka cilmi-baarista ee kaladuwan ama noocyada. -Ga / -dasilicoNaqshadeynta Hore ayaa loo sameeyaa si loo hubiyo qaabeynta enzyme ee ugu habboon.
● Waxqabadka sare ee dheefshiidka enzymaalka: Xamuulka asilicoNaqshadeynta Hore iyo Imtixaanka Hore ee loo yaqaan 'Hore'
●Khibrad ballaaran: Kooxdeenu waxay u keentaa qibrad badan oo ku saabsan mashruuc kasta, oo leh rikoor waddo ah oo lagu xirayo 5000 mashruuc oo SLF-SEQ ah, oo ay ku jiraan dhirta, shimbiraha, shimbiraha, cayayaanka, iyo noolaha.
● Is-soo-kicinta Shaqo-xumada Biiinfflow: BMKGenE waxay horumarisay shaqo-ku-meel-gaar ah oo isku dhafan oo loo yaqaan 'sal-hanashada saldhiga loo yaqaan' saldhiga 'Slaf-seq' si loo hubiyo isku halaynta iyo sax ahaanta soosaarka kama dambaysta ah.
Faahfaahinta adeegga
| Nooca falanqaynta | Qiyaasta shacabka ee lagu taliyay | Istaraatiijiyadda isku xigta | |
| Qoto dheer oo ah taxanaha sumadda | Lambarka TAG | ||
| Khariidadaha hidda-wadaha | 2 Waaliddiinta iyo> 150 farcan | Waaliddiinta: 20x WGS DIIWAANKA: 10x | Cabbirka Gedda: <400 MB: WGS waa lagu talinayaa <1GB: 100K calaamadaha 1-2GB :: 200K calaamadaha > 2GB: 300K calaamadaha Max 500K calaamadaha |
| Daraasaadka Ururka Genome-ballaaran (Gwas) | Muunadaha | 10x | |
| Evolution hidde | Saamballada muunadaha, oo leh> 10 saamba koox hoosaad kasta | 10x | |
Shuruudaha adeegga
U fiirsashada ≥ 5 ng / μl
Wadarta lacagta ≥ 80 ng
Nanodrop OD260 / 280 = 1.6-2.5
Agaresol Gel: Maya ama xadidan ama faddarayn
Keenista muunad
Weelka: 2 ml centrifuge tuubada tuubada
(Inta badan saamiyada, waxaan kugula talineynaa inaadan ka badbaadin ethanol)
Calaamadaha muunad: muunadaha waxay u baahan yihiin in si cad loogu calaamadeeyo oo isku mid ah in la soo gudbiyo foomka macluumaadka muunad tusaalaha ah.
Shixnadda: ICE-ICE: Saamiyada ayaa loo baahan yahay in lagu duubo bacaha marka hore laguna aasay baraf qalalan.
Shaqada adeegga
Muunad Qc
Tijaabada tijaabada
SLAF-Timement
Diyaarinta Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Waxaa ku jira falanqaynta soo socota:
- Isugeynta xogta Qc
- Horumarka Slaf TAG
Khariidadda si loo tixraaco tirinta Genome
Iyada oo aan la helin tixraaca Genime: Isku-darka
- Falanqaynta calaamadaha SLAF.: Tirakoobka, qeybinta guud ee Genome
- Marker daahfurka: SNP, SNP, Infel, SNV, CV wacitaan iyo akhlaaq xumo
Qaybinta calaamadaha Slaf ee koromosoomyada:
Qaybinta SNPs ee koromosoomyada:
Faahfaahinta SNP
| Sannad | Wargeys | IF | Cinwaan | Codsiyada |
| 2022 | Isgaarsiinta Dabeecadda | 17.694 | Aasaaska Genomic ee Giga-koromosoomyada iyo Giga-Giga-Genome of Peony Paeonia osta | Slaf-Gwas |
| 2015 | Phytorof-tayo cusub | 7.433 | Meelaha laga soo bilaabo xargaha xargaha majaajillada gobollada geeska ah ee muhiimadda aasaasiga ah ee soybeans | Slaf-Gwas |
| 2022 | Joornaalka cilmi baarista horumarsan | 12.822 | Geeraha Genime-oo ballaaran ee farshaxanka ee farshaxanka ee gospadudsense wuxuu galay G. Horsutumum Muuji Losi ISKI ee Hordhaca Isku mar ee tayada suuf ee suufka iyo dhalidda sidaar | Genepics Slaf-Evolucary |
| 2019 | Warshad molikalar | 10.81 | Falanqaynta Genomiyada ee dadka iyo Golaha Devo ee De Novo waxay muujinayaan asal ahaan Bariis ahaan ciyaar isbedel ah | Genepics Slaf-Evolucary |
| 2019 | Dabeecadaha dabiiciga ah | 31.616 | Isku xigxiga Genome iyo Kala-duwanaanta hidda-wadaha ee rooga caadiga ah, Cyprinum Cardio | Khariidadda Slaf-Link-Link |
| 2014 | Dabeecadaha dabiiciga ah | 25.455 | Penime of Peanut ee la beero waxay bixisaa fahamka lugta Legame Karyotypes, Polyploid Evolution iyo Goobta dalaga. | Khariidadda Slaf-Link-Link |
| 2022 | MADAXWEYNAHA PREGECHOLOLOGOLOLOLOLOLOLOLS | 9.803 | Aqoonsiga ST1 waxay muujineysaa xulkiisa ku lug leh hirtchhiking ee morphilogy iyo saliida saliida inta lagu gudajiro hantanka soybean | Horumarinta Slaf-Marker |
| 2022 | Joornaalka caalamiga ah ee cilmiga molikalar | 6.208 | Aqoonsiga iyo sumadeeyaha DNA-da ee horumarinta qamadiga ee Mollis 2ns (2D) Diyaarad koromosoom ah | Horumarinta Slaf-Marker |
| Sannad | Wargeys | IF | Cinwaan | Codsiyada |
| 2023 | Xuduudaha ee sayniska dhirta | 6.735 | Khariidadda Qtl iyo Falanqaynta Transcriport ee ku saabsan sonkorta sonkorta inta lagu gudajiro miro bisil ah ee Pyrus Pyrifolia | Khariidadda hiddo-wadaha |
| 2022 | MADAXWEYNAHA PREGECHOLOLOGOLOLOLOLOLOLOLS | 8.154 | Aqoonsiga ST1 waxay muujineysaa xulkiisa ku lug lahaanshaha muruqyada morphicikology iyo saliida saliida inta lagu gudajiray
| Wacitaanka SNP |
| 2022 | Xuduudaha ee sayniska dhirta | 6.623 | Khariidadda Ururka Genome-Arday ee Nasiibka ah ee jawiga aan caadiga ahayn ee jawiga abaarta.
| Gwas |
