Taxanaha Bisulfite ee la dhimay (RRBS)
Tilmaamaha Adeegga
Platform: NovaSeq platform, PE150
Nooca maktabadda: 200-400bp gelin bisulfite daaweeyay maktabadda DNA
Istaraatiijiyada isku xigxiga: Lammaanaha-Dhamaadka 150 bp
Soo saarista xogta lagu talinayo: 10 Gb xog cayriin/muunad
Shuruudaha Tusaalaha
Tirada DNA: ≥ 2ug
Heerarka DNA: ≥ 20ng/μl.
Daahirsanaanta: OD260/280 = 1.8 ilaa 2.0 iyada oo aan hoos u dhicin ama wasakhayn RNA
Falanqaynta bioinformatics
Socodka Shaqada Adeegga
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
a.Tixraaca is-waafajinta genome-ga
Tirakoobka khariidaynta ayaa akhriya
Stirakoobka isku xigxiga qoto dheer iyo daboolida goobta C
Acaymis urursan
Dwaxtarka dheefshiidka ee muunad kasta
b.Ogaanshaha goobta methylation
Liiska heerarka methylation ee goobta C
BBeddelka S
Mcytosine ethylated
Qaybinta heerka methylation
c.Khariidadda Methylation
Gballaaran
Gelement enome
d.Isbarbardhigga heerarka methylation ee u dhexeeya shaybaarada
PIsku xirka hawada ee CG methylation
PCA-CG methylation
Cdhaldhalaalka CG methylation
d.Falanqaynta methylation kala duwan
STusmada ugu hooseysa ee DMR
Qaybinta dhererka DMR
Hcun khariidad
DQaybinta MR ee gobolka la beegsaday
