● Ku darida Illumina NovaSeq oo leh PE150.
Adeeggu wuxuu u baahan yahay muunado unug, halkii laga soo saari lahaa asiidhyada nucleic-ka ah, si looga gudbo formaldehyde oo loo ilaaliyo isdhexgalka borotiinka DNA.
● Tijaabada Hi-C waxay ku lug leedahay xaddidaadda iyo dhammaadka hagaajinta cidhifyada dhegdhegaysan ee biotin, oo ay ku xigto wareegtaynta darafyada aan fiicneyn iyadoo la ilaalinayo isdhexgalka. DNA-da waxaa lagu soo dejiyaa kuulka streptavidin waxaana lagu nadiifiyaa diyaarinta maktabadda xigta.
Dulmarka Hi-C
(Lieberman-Aiden E et al.,Sayniska, 2009)
●Ciribtirka Baahida Xogta Dadweynaha Hiddaha:Hi-C waxay beddeshaa macluumaadka muhiimka ah ee looga baahan yahay joogteynta.
●Cufnaanta calaamade sare:taas oo horseedaysa in saamiga barkinta kontiig sare uu ka sarreeyo 90%.
●Khibrad ballaadhan iyo diiwaannada daabacaadda:BMKGene waxay leedahay waayo-aragnimo ballaaran oo leh in ka badan 2000 xaaladood oo Hi-C Genome Assembly oo ka socda 1000 nooc oo kala duwan iyo shatiyo kala duwan. In ka badan 200 oo kiis oo la daabacay ayaa leh saameyn urursan oo ka badan 2000.
●Kooxda bioinformatics ee xirfad sare leh:iyada oo leh shatiyada guriga iyo xuquuqda daabacaadda software-ka ee tijaabooyinka Hi-C iyo falanqaynta xogta, software-ka xogta aragga ee is-horumariyay ayaa awood u siinaya xannibaadda gacanta ku dhaqaaqista, rogida, burinta, iyo dib u samaynta.
●Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
●Tibaaxo Dhamaystiran: waxaan isticmaalnaa xog uruurin badan si aan si shaqeyn leh ugu sharaxno hiddo-wadaha kala duwanaansho la aqoonsaday oo aan sameyno falanqaynta kobcinta u dhigma, anagoo bixinayna fikrado ku saabsan mashruucyo cilmi baaris oo badan.
Diyaarinta maktabadda | Istaraatiijiyada isku xigxiga | Soo saarista xogta lagu taliyay | Xakamaynta tayada |
Hi-C maktabadda | Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Nudaha | Qadarka loo baahan yahay |
Viscera xoolaha | ≥ 2 g |
Murqaha Xoolaha | |
Dhiiga naasleyda | ≥ 2 ml |
Dhiiga digaaga/Kalluunka | |
Dhirta- Caleen Cusub | ≥ 3 g |
Unugyada Dhaqanka | ≥ 1x107 |
Cayayaanka | ≥ 2 g |
1) Xogta ceeriin QC
2) Maktabadda Hi-C QC: qiyaasta isdhexgalka Hi-C ee ansax ah
3) Golaha Hi-C: ururinta kontiig kooxeedyo, oo ay ku xigto in koox walba la isku diro oo loo sameeyo jihaynta contig
4) Qiimaynta Hi-C
Maktabadda Hi-C QC - qiyaasta Hi-C lammaane is dhexgalka ansax ah
Golaha Hi-C - tirakoobyada
Qiimaynta shirka ka dib - khariidadda kulaylka ee xoojinta calaamadaha u dhexeeya qashinka
Sahami horumarada ay fududaysay BMKGene's Hi-C adeegyada isu-ururinta daabacaadyada.
Tian, T. iyo al. (2023) 'Geome-ga ururinta iyo kala qaybinta hidde-sidaha ee jeermiska galleyda abaarta u adkeysta ee caanka ah', Nature Genetics 2023 55:3, 55(3), bogga. 496-506. doi: 10.1038/s41588-023-01297-y.
Wang, ZL iyo al. (2020) 'Golaha Miisaanka Chromosome-ka ee Asian Honeybee Apis cerana Genome', Xudduudaha ku jira Hiddaha, 11, b. 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.
Zhang, F. iyo al. (2023) 'Daah furka horumarka tropane alkaloid biosynthesis iyadoo la falanqeynayo laba genomes ee qoyska Solanaceae', Isgaarsiinta Dabeecadda 2023 14:1, 14(1), pp. 1-18. doi: 10.1038/s41467-023-37133-4.
Zhang, X. iyo al. (2020) 'Genomes of the Banyan Tree and pollinator Wasp' Waxay bixiyaan aragtiyo ku saabsan berdaha-wasp Coevolution', Cell, 183(4), bogga 875-889.e17. doi: 10.1016/J.CELL.2020.09.043