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Alaabta

Hi-C Ku Salaysan Golaha Genome

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Hi-C waa hab loogu talagalay in lagu qabto qaabeynta koromosoomyada iyadoo la isku darayo isdhexgalka ku saleysan dhowaanshaha iyo isku xigxiga sareynta. Xoogga is dhexgalkaan ayaa la rumeysan yahay inay si xun ula xiriirto fogaanta jireed ee koromosoomyada. Sidaa darteed, xogta Hi-C waxa loo isticmaalaa in lagu hago ururinta, dalbashada, iyo jihaynta taxanaha la isku keenay ee hiddo-wadaha qabyada ah iyo ku xidhidhiyeyaasha tiro cayiman oo koromosoomyo ah. Farsamadani waxay awood siinaysaa isu imaatinka heerka koromosoomyada marka ay maqan tahay khariidad hidde-wadaaga ku salaysan. Genom kasta wuxuu u baahan yahay Hi-C.


Faahfaahinta Adeegga

Bioinformatics

Natiijooyinka Demo

Daabacaada sifada

Tilmaamaha Adeegga

● Ku darida Illumina NovaSeq oo leh PE150.

Adeeggu wuxuu u baahan yahay muunado unug, halkii laga soo saari lahaa asiidhyada nucleic-ka ah, si looga gudbo formaldehyde oo loo ilaaliyo isdhexgalka borotiinka DNA.

● Tijaabada Hi-C waxay ku lug leedahay xaddidaadda iyo dhammaadka hagaajinta cidhifyada dhegdhegaysan ee biotin, oo ay ku xigto wareegtaynta darafyada aan fiicneyn iyadoo la ilaalinayo isdhexgalka. DNA-da waxaa lagu soo dejiyaa kuulka streptavidin waxaana lagu nadiifiyaa diyaarinta maktabadda xigta.

Faa'iidooyinka Adeegga

1 Mabda'a-Hi-C-isku-xigta

Dulmarka Hi-C
(Lieberman-Aiden E et al.,Sayniska, 2009)

Ciribtirka Baahida Xogta Dadweynaha Hiddaha:Hi-C waxay beddeshaa macluumaadka muhiimka ah ee looga baahan yahay joogteynta.

Cufnaanta calaamade sare:taas oo horseedaysa in saamiga barkinta kontiig sare uu ka sarreeyo 90%.

Khibrad ballaadhan iyo diiwaannada daabacaadda:BMKGene waxay leedahay waayo-aragnimo ballaaran oo leh in ka badan 2000 xaaladood oo Hi-C Genome Assembly oo ka socda 1000 nooc oo kala duwan iyo shatiyo kala duwan. In ka badan 200 oo kiis oo la daabacay ayaa leh saameyn urursan oo ka badan 2000.

Kooxda bioinformatics ee xirfad sare leh:iyada oo leh shatiyada guriga iyo xuquuqda daabacaadda software-ka ee tijaabooyinka Hi-C iyo falanqaynta xogta, software-ka xogta aragga ee is-horumariyay ayaa awood u siinaya xannibaadda gacanta ku dhaqaaqista, rogida, burinta, iyo dib u samaynta.

Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.

Tibaaxo Dhamaystiran: waxaan isticmaalnaa xog uruurin badan si aan si shaqeyn leh ugu sharaxno hiddo-wadaha kala duwanaansho la aqoonsaday oo aan sameyno falanqaynta kobcinta u dhigma, anagoo bixinayna fikrado ku saabsan mashruucyo cilmi baaris oo badan.

Tilmaamaha Adeegga

Diyaarinta maktabadda

Istaraatiijiyada isku xigxiga

Soo saarista xogta lagu taliyay

Xakamaynta tayada

Hi-C maktabadda

Illumina NovaSeq PE150

100x

Q30 ≥ 85%

Shuruudaha Tusaalaha

Nudaha

Qadarka loo baahan yahay

Viscera xoolaha

≥ 2 g

Murqaha Xoolaha

Dhiiga naasleyda

≥ 2 ml

Dhiiga digaaga/Kalluunka

Dhirta- Caleen Cusub

≥ 3 g

Unugyada Dhaqanka

≥ 1x107

Cayayaanka

≥ 2 g

Socodka Shaqada Adeegga

Tusaalaha QC

Naqshadaynta tijaabada

muunad keenid

Tusaalaha keenista

Diyaarinta Maktabadda

Dhismaha maktabadda

Isku xigxiga

Isku xigxiga

Falanqaynta xogta

Falanqaynta xogta

Adeegyada iibka kadib

Adeegyada iibka kadib


  • Kii hore:
  • Xiga:

  • 流程图 羽莹-01

    1) Xogta ceeriin QC

    2) Maktabadda Hi-C QC: qiyaasta isdhexgalka Hi-C ee ansax ah

    3) Golaha Hi-C: ururinta kontiig kooxeedyo, oo ay ku xigto in koox walba la isku diro oo loo sameeyo jihaynta contig

    4) Qiimaynta Hi-C

    Maktabadda Hi-C QC - qiyaasta Hi-C lammaane is dhexgalka ansax ah

     

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    Golaha Hi-C - tirakoobyada

     

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    Qiimaynta shirka ka dib - khariidadda kulaylka ee xoojinta calaamadaha u dhexeeya qashinka

     

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    Sahami horumarada ay fududaysay BMKGene's Hi-C adeegyada isu-ururinta daabacaadyada.

    Tian, ​​T. iyo al. (2023) 'Geome-ga ururinta iyo kala qaybinta hidde-sidaha ee jeermiska galleyda abaarta u adkeysta ee caanka ah', Nature Genetics 2023 55:3, 55(3), bogga. 496-506. doi: 10.1038/s41588-023-01297-y.

    Wang, ZL iyo al. (2020) 'Golaha Miisaanka Chromosome-ka ee Asian Honeybee Apis cerana Genome', Xudduudaha ku jira Hiddaha, 11, b. 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.

    Zhang, F. iyo al. (2023) 'Daah furka horumarka tropane alkaloid biosynthesis iyadoo la falanqeynayo laba genomes ee qoyska Solanaceae', Isgaarsiinta Dabeecadda 2023 14:1, 14(1), pp. 1-18. doi: 10.1038/s41467-023-37133-4.

    Zhang, X. iyo al. (2020) 'Genomes of the Banyan Tree and pollinator Wasp' Waxay bixiyaan aragtiyo ku saabsan berdaha-wasp Coevolution', Cell, 183(4), bogga 875-889.e17. doi: 10.1016/J.CELL.2020.09.043

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