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Alaabta

circRNA sequencing-Illumina

Isku xigxiga RNA wareegtada ah (circRNA-seq) waa in la soo bandhigo oo la falanqeeyo RNA-yada wareegsan, koox ka mid ah molecules RNA kuwaas oo sameeya wareegyo xiran oo ay ugu wacan tahay dhacdooyinka kala-baxa ee aan kanoniga ahayn, taasoo siinaysa RNA-gan xasilooni korodhay.Iyadoo qaar ka mid ah circRNA-yada la muujiyey inay u dhaqmaan sidii isbuunyo microRNA ah, kala soocida microRNA-yada oo laga hortagayo inay nidaamiyaan mRNA-yada bartilmaameedka ah, circRNA-yada kale ayaa laga yaabaa inay la falgalaan borotiinnada, beddelaan muujinta hidda-wadaha, ama waxay door ku leeyihiin hababka gacanta.Falanqaynta muujinta circRNA waxay bixisaa aragtiyo ku saabsan doorarka nidaaminta unugyadan iyo muhiimada ay ku leeyihiin hababka kala duwan ee unugyada, heerarka korriinka, iyo xaaladaha cudurka, taasoo gacan ka geysaneysa faham qoto dheer oo ku saabsan kakanaanta nidaamka RNA ee macnaha guud ee muujinta hidda-wadaha.


Faahfaahinta Adeegga

Bioinformatics

Natiijooyinka Demo

Daabacaada sifada

Astaamaha

● Qalafsanaanta rRNA oo ay ku xigto diyaarinta maktabadda jihada, taasoo awood u siinaysa xogta isku xigxiga ee u gaarka ah.

● Socod-shaqeedka bioinformatic wuxuu awood u siinayaa saadaasha circRNA iyo cabbirka muujinta

 

Faa'iidooyinka Adeegga

Maktabadaha RNA oo dhamaystiran:Waxaan isticmaalnaa dhimista rRNA halkii aan ka isticmaali lahayn dhimista RNA toosan diyaarintayada maktabadda ka hor, hubinta in xogta taxanaha ah ay ku jiraan kaliya maahan circRNA laakiin sidoo kale mRNA iyo lncRNA, taas oo awood u siinaysa falanqaynta wadajirka ah ee xogtan

Falanqaynta ikhtiyaariga ah ee shabakadaha RNA (ceRNA) ee tartanka ku jira: bixinta aragti qoto dheer oo ku saabsan hababka nidaaminta gacanta

Khibrad Balaadhan: iyada oo leh rikoodh raad-raac ah oo lagu farsameeyo in ka badan 20,000 oo muunado BMK ah, oo ku baahsan noocyo kala duwan oo muunado iyo mashruucyo lncRNA ah, kooxdeenu waxay u keentaa khibrad badan mashruuc kasta.

Xakamaynta tayada adagWaxaan hirgelineynaa qodobbada kontoroolka asaasiga ah ee dhammaan heerarka, laga bilaabo muunad iyo diyaarinta maktabadda ilaa isku xigxiga iyo bioinformatics.Kormeerkan taxaddar leh wuxuu xaqiijinayaa gaarsiinta natiijooyin tayo sare leh oo joogto ah.

● Taageerada iibka kadibBallanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib.Inta lagu jiro wakhtigan, waxaan bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto su'aalo kasta oo la xidhiidha natiijooyinka.

Tusaalaha Shuruudaha iyo Bixinta

Maktabadda

Platform

Xogta lagu taliyay

Xogta QC

Poly A waa la hodmay

Illumina PE150

16-20 Gb

Q30≥85%

Tusaalaha Shuruudaha:

Nucleotides:

Conc.(ng/μl)

Qadarka (μg)

daahirnimo

Daacadnimo

≥ 100

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Xaddidan ama aan lahayn borotiin ama wasakh DNA oo lagu muujiyey jel.

Dhirta: RIN≥6.5;

Xoolaha: RIN≥7.0;

5.0≥28S/18S≥1.0;

xaddidan ama aan lahayn sare u qaadis aasaasi ah

● Dhirta:

Xididka, stem ama Petal: 450 mg

Caleen ama abuur: 300 mg

Miraha: 1.2 g

● Xoolaha:

Wadnaha ama mindhicirka: 450 mg

Viscera ama Maskaxda: 240 mg

Murqaha: 600 mg

Lafaha, timaha ama maqaarka: 1.5g

● Arthropods:

Cayayaanka: 9g

Crustacea: 450 mg

● Dhiig dhan:2 tuubbo

Unugyada: 106 unugyo

● Serum iyo Plasma: 6 ml

Tusaalaha keenista lagu taliyay

Weel: 2 ml oo tuubo sentrifuge ah (Faylada qasacadaysan laguma talinayo)

Tusaalaha calaamadaynta: Kooxda+ku celcelinta tusaale A1, A2, A3;B1, B2, B3......

Raritaanka:

1. Barafka qallalan: Muunadodu waxay u baahan yihiin in lagu xidho bac oo lagu aaso baraf qallalan.

2. Tuubooyinka RNAstable: Muunada RNA waxaa lagu qallajin karaa tuubada xasilinta RNA (tusaale RNAstable®) waxaana lagu rari karaa heerkulka qolka.

Socodka Shaqada Adeegga

Tusaalaha QC

Naqshadaynta tijaabada

muunad keenid

Tusaalaha keenista

Tijaabada tijaabada

soo saarida RNA

Diyaarinta Maktabadda

Dhismaha maktabadda

Diyaarinta Maktabadda

Isku xigxiga

Falanqaynta xogta

Falanqaynta xogta

Adeegyada iibka kadib

Adeegyada iibka kadib


  • Hore:
  • Xiga:

  • Bioinformatics

    wp_doc_15

    saadaasha circRNA: qaybinta koromosoomyada

     图片36

     

    circRNA-yada si ka duwan loo soo bandhigay - Dhulkaanaha

     图片37

     

    Si ka duwan loo soo bandhigay circRNA-yada - kooxaynta kala sareynta

     图片38

     

    Kobcinta shaqaynta ee hiddo-wadaha martida loo yahay ee circRNA

     图片39

     

     

    Sahami horumarka cilmi-baarista ee ay fududeeyeen adeegyada isku xigxiga ee BMKGene' circRNA iyada oo loo marayo ururinta daabacaadyo la soo xulay.

     

    Wang, X. iyo al.(2021) 'CPSF4 waxay xakameysaa samaynta circRNA iyo microRNA dhexdhexaadinta hiddo-wadaha aamusnaanta hepatocellular carcinoma', Oncogene 2021 40:25, 40(25), bogga. 4338-4351.doi: 10.1038/s41388-021-01867-6.

    Xia, K. iyo al.(2023) 'X oo-jawaab qoraal ah ayaa daaha ka qaadaya doorka wareegtada RNA133 ee u adkeysiga cudurrada iyadoo la xakameynayo muujinta OsARAB ee bariiska', Cilmi-baarista Phytopathology, 5(1), bogga 1-14.doi: 10.1186/S42483-023-00188-8/SAWIRRO/6.

    Y, H. iyo al.(2023) 'CPSF3 waxay beddeshaa dheelitirka qoraallada wareegta ah iyo kuwa toosan ee kansarka hepatocellular.doi: 10.21203/RS.3.RS-2418311/V1.

    Zhang, Y. iyo al.(2023) 'Qiimayn dhamaystiran oo cirrno ah oo ku jira cirrhotic cardiomyopathy ka hor iyo ka dib beerka bedelka', International Immunopharmacology, 114, p.109495. doi: 10.1016/J.INTIMP.2022.109495.

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