CircrNa Isu-duwaha
Astaamaha
● Qalliinka RRNA waxaa ku xigay diyaarinta maktabada jihada, karti u yeelashada xogta xididdada gaarka ah ee xididka.
● Boodhfflowlowlowlowglowflowfffflowsflow waxay u sahlaysaa saadaalinta Circrna iyo tirinta muujinta
Faa'iidooyinka adeegga
●Maktabadaha RNA ee dhameystiran ee RNA:Waxaan u isticmaalnaa Rrna debletin halkii aan ka heli lahayn dena diyaarinta maktabadda ee maktabadda, hubinta in xogta isku xirka ay kujirto kaliya Circre laakiin sidoo kale Mrna iyo Lancna, oo awood u siinaya falanqaynta wadajirka ah ee ku saabsan xog-ururintan
●Falanqaynta ikhtiyaariga ah ee ku saabsan shabakadaha loo tartamayo ee rnenous (Cerma): Bixinta faham qoto dheer oo ku saabsan farsamooyinka sharci-dejinta ee unugyada
●Khibrad ballaaran: Iyada oo la raacayo diiwaan-galinta wadooyinka ee ka badan 20,000 oo muunadood oo ka badan BMKGene, oo ay soo saarto noocyo muunad oo kala duwan iyo mashruucyo Lancna, kooxdeenu waxay u keentaa khibrad badan oo ku saabsan mashruuc kasta.
●Xakamaynta tayada adag ee adag: Waxaan ka hirgelinaa qodobbada kontoroolka core ee dhammaan marxaladaha, tusaale ahaan muunadda iyo u diyaarinta maktabadda si ay u helaan isku xigxiga iyo bioinformatics. Korjoogteyntan sifiican loo kormeer ayaa hubiya gaarsiinta natiijooyin tayo sare leh oo joogto ah.
● Taageerada iibka ka dib: Balankeenna wuxuu ku fidsan yahay dhameystirka mashruuca ee leh muddo 3 bilood iibka iibka ah ka dib. Inta lagu jiro waqtigan, waxaan bixinaa dabagal dabagal mashruuc, gargaar dhibaatayn, iyo Q & SS & Kalfadhiyo si wax looga qabto wixii su'aalo ah ee la xiriira natiijooyinka.
Shuruudaha muunadda iyo dhalmada
| Maktabad | Boosteejo | Xogta lagu taliyay | XIGASHADA QC |
| RrNa dhammaatay Maktabadda Joogtada ah ee RRNA | Iftiimina PE150 | 16-20 GB | Q3t85% |
Shuruudaha muunadda:
Nucleotatides:
| CODKA. (NG / μL) | Tirada (μg) | Daahir | Sharaf |
| 80 | ≥ 0.8 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Rin≥6.0; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
Dhirta:
Xidid, asliga ah ama petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
● Xayawaanka:
Wadnaha ama xiidmaha: 450 mg
Viscera ama maskaxda: 240 mg
Murqaha: 600 mg
Lafaha, timaha ama maqaarka: 1.5g
● Arthropods:
Cayayaanka: 9g
Crustacea: 450 mg
Dhiiga oo dhan:2 tuubooyin
● Unugyada: 106 unugyada
● Serum iyo plasma: 6 ml
Keenista muunad
Weelka: 2 ml centrifouge tuubada (foil tin tin laguma talinayo)
Calaamadaha muujinta: Kooxda + waxay ku daydaa tusaale ahaan a1, A2, A3; B1, B2, B3.
Shixnad:
1. Ice-ICE: Saamiyada ayaa loo baahan yahay in lagu buuxiyo bacaha oo lagu aasay baraf qalalan.
2. Tuunyo aan la qiimeyn karin: Muunadaha RNA-da ayaa lagu qallajin karaa tuubbooyinka xasilinta ee RNA (tusaale, rnastable®) oo lagu ridayaa heerkulka qolka.
Socodka shaqada adeegga
Naqshadeynta Tijaabada
Keenista muunad
Soo saarida RNA
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Bioinformatics
Saadaalinta Circrena: qaybinta koromosoomyada
Si kala sooc ah u muujiyey Circrnas - Prodcano Plant
Si kala duwan u muujiyey Circrnas - isku-darka hantida
Kobcinta howsha hidda-wadaha ee Circrna
Baadh horumarka cilmi baarista ee ay fududeeyaan BMKGene 'Circre Services Sconcention Scoenking' iyada oo loo marayo aruurinta daaqsinta daabacaadaha.
Wang, x. et al. (2021) 'CPSF4 waxay nidaamisaa dhismaha Circrena oo ay ku dhexjirtay aamusnaanta hidda-wadeyaasha ee ku yaal hepatocellular carcinoma', oncogene 2021 40), pp. 4338-4351. doi: 10.1038 / s41388-021-01867-6.
Xia, k. et al. (2023) 'X oo ah Jawaabta Jawaabta waxay muujineysaa doorka wareegga RNA133 ee loo yaqaan' Resespict rna 'oo lagu habeeyo muujinta dhawaaqa Osarab ee bariiska', cilmi baarista phytopatology, 1 (1), pp. 1-14. DI: 10.11186 / S42483-023-00188-8 / Jaantusyada / 6.
Y, H. et al. (2023) 'CPSF3 waxay qayb ka qaadataa isku-dheelitirka wareegga wareegga iyo tooska ah ee ku saabsan qoraalada laf-dhabarta ee buugga' hepatocellular ''. doi: 10.21203 / Rs.3.RS-2418311 / V1.
Zhang, y. et al. (2023) Qiimaynta dhameystiran ee Circrnas ee Cirridnas Cirridyopathy ka hor iyo ka dib beerka ka-tallaalidda beerka ', Tartanka Caalamiga ah ee Jinlootiga, 114, P. 109495. Do: 10.1016 / j.intifte.2022.109495.
