Isku xigxiga qoraalka oo dhan - Illumina
Astaamaha
● Labada maktabadood si ay isugu xig-xigaan qoraalka qoraalka oo dhammaystiran: rRNA-yarida oo ay ku xigto diyaarinta maktabadda PE150 iyo xulashada cabbirka oo ay ku xigto diyaarinta maktabadda SE50
● Falanqaynta bioinformatics oo dhammaystiran ee mRNA, lncRNA, circRNA iyo miRNA ee warbixinnada bioinformatics ee kala duwan
● Falanqaynta wadajirka ah ee dhammaan tibaaxaha RNA ee warbixinta la isku daray, oo ay ku jiraan falanqaynta shabakadaha ceRNA.
Faa'iidooyinka Adeegga
●Falanqaynta qoto dheer ee shabakadaha sharciyeyntaFalanqaynta shabakada ceRNA waxaa awood u leh isku xigxiga wadajirka ah ee mRNA, lncRNA, circRNA iyo miRNA iyo socodka shaqada bioinformatic oo dhamaystiran.
●Tibaaxo DhamaystiranWaxaan isticmaalnaa xog uruurin badan si aan si shaqeyn leh u qeexno Hidde-yaasha kala duwan (DEGs) oo aan sameyno falanqaynta kobcinta ee u dhiganta, siinta fikrado ku saabsan hababka gacanta iyo molecular ee ka hooseeya jawaabta qoraalka.
●Khibrad Balaadhan: iyada oo si guul leh loo xidhay in ka badan 2000 mashruucyo qoraal ah oo kala duwan oo cilmi baaris ah, kooxdeenu waxay u keentaa khibrad badan mashruuc kasta.
●Xakamaynta tayada adagWaxaan hirgelineynaa qodobbada kontoroolka asaasiga ah ee dhammaan heerarka, laga bilaabo muunad iyo diyaarinta maktabadda ilaa isku xigxiga iyo bioinformatics.Kormeerkan taxaddar leh wuxuu xaqiijinayaa gaarsiinta natiijooyin tayo sare leh oo joogto ah.
● Qoraal DhameystiranWaxaan isticmaalnaa xog uruurin badan si aan si shaqeyn leh u qeexno Hidde-yaasha kala duwan (DEGs) oo aan sameyno falanqaynta kobcinta ee u dhiganta, siinta fikrado ku saabsan hababka gacanta iyo molecular ee ka hooseeya jawaabta qoraalka.
●Taageerada Iibka KadibBallanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib.Inta lagu jiro wakhtigan, waxaan bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto su'aalo kasta oo la xidhiidha natiijooyinka
Tusaalaha Shuruudaha iyo Bixinta
| Maktabadda | Istaraatiijiyada isku xigxiga | Xogta lagu taliyay | Xakamaynta tayada |
| rRNA yaraatay | Illumina PE150 | 16 Gb | Q30≥85% |
| Cabbirka la doortay | Illumina SE50 | 10-20M ayaa akhriya |
Tusaalaha Shuruudaha:
Nucleotides:
| Conc.(ng/μl) | Qadarka (μg) | daahirnimo | Daacadnimo |
| ≥ 100 | ≥ 1 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Xaddidan ama aan lahayn borotiin ama wasakh DNA oo lagu muujiyey jel. | Dhirta: RIN≥6.5 Xayawaanka: RIN≥7.0 5.0≥28S/18S≥1.0; xaddidan ama aan lahayn sare u qaadis aasaasi ah |
Tusaalaha keenista lagu taliyay
Konteenar:
2 ml oo tuubo sentrifuge ah (Faylada qasacadaysan laguma talinayo)
Tusaalaha calaamadaynta: Kooxda+ku celcelinta tusaale A1, A2, A3;B1, B2, B3......
Raritaanka:
1.Baraf qallalan: Tijaabooyinku waxay u baahan yihiin in lagu xidho bacaha oo lagu aaso baraf qallalan.
Tubooyinka RNAstable: Muunada RNA waxaa lagu qallajin karaa tuubada xasilinta RNA (tusaale RNAstable®) waxaana lagu rari karaa heerkulka qolka.
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
soo saarida RNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Bioinformatics
dulmar muujinta RNA
Hiddo-sidaha si ka duwan loo soo bandhigay
falanqaynta ceRNA
MiRNA-yada si kala duwan loo muujiyay iyo RNA-yada la xidhiidha
Sahami horumarka cilmi-baadhiseed ee ay fududaysay BMKGene dhammaan adeegyada isku xigxiga qoraalka qoraalka ah iyada oo loo marayo ururinta daabacaado la soo xulay.
Dai, Y. iyo al.(2022) 'Qaybaha odhaah dhamaystiran ee mRNAs, lncRNAs iyo miRNAs ee cudurka Kashin-Beck ee lagu aqoonsaday RNA-sequencing', Molecular Omics, 18(2), bogga 154-166.doi: 10.1039/D1MO00370D.
Liu, N. nan et al.(2022) 'Gene, 830, bogga. 146503-146503.doi: 10.1016/J.GENE.2022.146503.
Wang, XJ iyo al.(2022) 'Is-dhexgalka-Multi-Omics-ku Salaysan Mudnaanta ee Ku Tartamaya Shabakadaha Xeerka RNA ee ku jira Kansarka Sambabada Unugyada Yar: Tilmaamaha Molecular iyo Murashaxiinta Maandooriyaha', Frontiers in Oncology, 12, p.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al.(2022) 'Falanqaynta isku dhafan ee profiles-lncRNA/circRNA-miRNA-mRNA waxay muujinaysaa fikrado cusub oo ku saabsan hababka suurtagalka ah ee ka jawaab celinta nematodes-ka xididdada ee lawska', BMC Genomics, 23(1), pp. 1-12.doi: 10.1186/S12864-022-08470-3/SAWIRRO/7.
Yan, Z. iyo al.(2022) 'Taxane guud oo qoraal ah RNA wuxuu muujinayaa hababka molecular ee la xidhiidha dayactirka tayada goosashada ka dib ee brokoli ee iftiinka LED-ka cas', Bayoloji iyo Tignoolajiyada Dib-u-soo-saarka, 188, p.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
