Xilliga dhererka buuxa ee MRNA
Astaamaha
● Qabsashada poly-a-Mrna waxaa ku xigay CDNNA SCRITESIS iyo diyaarinta maktabadda
● Isku xigxiga waraaqaha dhererka buuxa
Falanqaynta bioinfformatic ee ku saleysan isku-darka tixraaca geedka
Falanqaynta bioinffratic-ga waxaa ka mid ah muujinta oo keliya ee hidda-wadaha iyo hiddo-wadeyaasha, sidoo kale falanqaynta LNCRA, Foosha-qalooca ee loo yaqaan 'Feeeln', Proy-Adcelytion
Faa'iidooyinka adeegga
●Tirada muujinta ee heerka Isomorm: Awood u yeelashada falanqaynta muujinta oo sax ah oo sax ah, isbeddel ka soo horjeedda oo laga yaabo in la dabooli karo markii la falanqayo muujinta hiddaha oo dhan
●Baahida xogta ee la dhimay:Marka la barbardhigo xisbiyada soo socda (NGS), isku xirka nanopore ayaa muujinaya shuruudo yar oo xog ah, oo u oggolaanaya heerar u dhigma oo u dhiganta fadhiyada xaraashka ah ee xogta yar.
●Sax ahaanta sare ee tirinta muujinta: Labadaba heerka hidda-wadaha iyo hiddo-wadaha
●Aqoonsiga macluumaad dheeri ah: Beddelka plandeyaderinylation-ka kale, fulinta hidda-wadaha iyo LCNRNRNA iyo hiddo-wadaha
●Khibrad ballaaran: Kooxdayadu waxay u keentaa waayo-aragnimo badan oo ku saabsan mashruuc kasta, oo dhammeystiray wax ka badan 850 mashruuc oo ah mashaariic kooban oo loo qoondeeyay oo ay ka shaqeeyaan wax ka badan 8,000 oo muunado ah.
●Taageerada iibka ka dib: Balankeenna wuxuu ku fidsan yahay dhameystirka mashruuca ee leh muddo 3 bilood iibka iibka ah ka dib. Inta lagu jiro waqtigan, waxaan bixinaa dabagal dabagal mashruuc, gargaar dhibaatayn, iyo Q & SS & Kalfadhiyo si wax looga qabto wixii su'aalo ah ee la xiriira natiijooyinka.
Shuruudaha muunadda iyo dhalmada
| Maktabad | Istaraatiijiyadda isku xigta | Xogta lagu taliyay | Xakamaynta Tayada |
| Poly si fiican | Iftiimina PE150 | 6/12 GB | Celcelis ahaan celcelis ahaan tayada tayada: Q10 |
Shuruudaha muunadda:
Nucleotatides:
| CODKA. (NG / μL) | Tirada (μg) | Daahir | Sharaf |
| ≥ 100 | ≥ 1.0 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Dhirta: Rin≥7.0; Xayawaanka: Rin≥7.5; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
Dhirta:
Xidid, asliga ah ama petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
● Xayawaanka:
Wadnaha ama xiidmaha: 300 mg
Viscera ama maskaxda: 240 mg
Murqaha: 450 mg
Lafaha, timaha ama maqaarka: 1g
● Arthropods:
Cayayaanka: 6g
Crustacea: 300 mg
Dhiiga oo dhan: 1 tuubbo
● Unugyada: 106 unugyada
Keenista muunad
Weelka: 2 ml centrifouge tuubada (foil tin tin laguma talinayo)
Calaamadaha muujinta: Kooxda + waxay ku daydaa tusaale ahaan a1, A2, A3; B1, B2, B3.
Shixnad:
1. Ice-ICE: Saamiyada ayaa loo baahan yahay in lagu buuxiyo bacaha oo lagu aasay baraf qalalan.
2. Tuunnada lagu qurxiyo
Socodka shaqada adeegga
Nucleotatides:
Keenista muunad
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Socodka shaqada adeegga
Unugyada:
Naqshadeynta Tijaabada
Keenista muunad
Soo saarida RNA
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
● Xogta xogta ceeriin
● Aqoonsiga qoraalka ah
● Kala-qaybin kale
● Tirada tibaabinta heerka hidda-wadaha iyo heerka imoform
● falanqaynta muujinta kala-sooca
● Faahfaahinta shaqeynta iyo kobcinta (degs iyo pros)
Falanqaynta Kala-sooca Kala-soocidda
Falanqaynta kale ee plandeyadenynyelylation (APA)
Saadaalinta LNCRNA
Faahfaahinta hiddaha cusub
Ku-darista BRES
Shabakadaha borotiinka-borotiinka borotiinka ee ku jira DEEGs
Sahaminta Horumarinta ay fududeysay Bmkgene ee Nannopore ee Adeegyada Dhexe ee Qaab-damiska M M Mrna ee Isuduwaha ee loo maro aruurinta daaqsinta daabacaadaha.
Gong, B. et al. (2023) firfircoonaanta epidenoe iyo firfircoonida ee qarkacaysa ee Kinse-ka ah ee Kinse-ka ah ee 'Comcogene' oo ah oncogene ee gloma ', joornaalka hidda-galka iyo setros, 50 (6), pp. doi: 10.1016 / j.Jgg.2023.01.008.
Isaga, z. et al. (2023) 'Tuspitocpitoc-ka dhererka buuxa ee dareeraha qanjidhada ayaa ka jawaabaya jawaabta ugu dhaqma ee Th1-lindures ee qulqulka (Paralichthys Oliveus)', Kalluunka Olicologs, 134, p. 108636. Do: 10.1016 / J.Fsi.2023.108336.
MA, y. et al. (2023) Falanqaynta Isbarbardhiga Pacio iyo Hababka Isku-darka ee Jnacyada ee loo yaqaan 'Nebopilama noterai', Genomas, 115 (6), p. 110709. DOI: 10.1016 / J.ygego.2023.110709.
Yu, d. et al. (2023) Falanqaynta Nano-Seq waxay shaaca ka qaadaysaa udub dhexaadka u dhexeeya walxaha iyo microvessics-ka iyo microvessicles-ka iyo ka soo baxa Humsc ', cilmi baarista unugyada ee asliga ah iyo daaweynta, 14 (1), pp. 1-13. doi: 10.11186 / s13287-023-0391-5 / miisaska / 6.
