Exploring gene expression patterns at the original location of an organism is crucial for understanding the types and functions of its cells. However, current spatial transcriptome analysis methods have limitations such as low throughput or insufficient resolution. The BMKMANU S1000 Spatial Chip, developed by BMKMANU, can detect in-situ gene expression information in complete tissue sections at subcellular resolution, enabling a nuanced interpretation of tissue structure.
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BMKMANU S1000 spatial chip and matching reagent kit includes:
1) Tissue Optimization Kit, containing reagents for tissue optimization (optimal time exploration for tissue permeabilization).
2) Gene Expression Kit, containing reagents for capturing mRNA in tissue slices for subsequent library preparation and sequencing.
3) Startup Kit: Includes a thermostatic adapter and a transcriptome magnetic separator.
The chip is based on micropores and microbeads, and mRNA can be captured in situ through Oligo on the microbeads. The Oligo is composed of four parts: Read1, Spatial Barcode, UMI, and Poly(dT)VN. After the tissue is attached to the chip, mRNA is released from the tissue by a permeabilization enzyme. Since most mRNA 3' ends have a Poly-A tail, they are captured by Oligo with Poly(dT)VN. After RT-PCR and cDNA amplification, library preparation and sequencing, spatial position tracing is achieved through Spatial Barcode. This allows for gene expression analysis in spatial locations and tissue heterogeneity.
It can be applied to most areas of biological and medical research, including tumor, disease, immunology, and developmental biology, helping to achieve new breakthroughs in these fields with spatial expression analysis at subcellular resolution.
●Tumor and Disease:
Spatial heterogeneity and microenvironment of tumors and diseases
Onset and development of tumors and diseases
Treatment response of tumors and diseases
Spatiotemporal atlas of organs
Gene regulatory mechanisms during development
Biotic stress response
Abiotic stress response
Immune response in organ transplantation
Immune mechanisms of tumors and diseases
Pathogenesis of autoimmune diseases
●Drug resistance analysis
Mechanisms of drug resistance
Research and development of new drugs
Cases and Demo Data
BMKMANU S1000 has been performance-verified in hundreds of cases across different tissue types.