Tixraaca aan tixraacin ku saleysan Mrna
Astaamaha
● Qabsashada poly Mrna ka hor diyaarinta maktabadda
● Waxay ka madax banaanayaan gole kasta oo GOMOFOme: oo ku saleysan Golaha Maqaarka Devo: Abuuritaanka liiska Hatabas-ka ee lagu sharaxay xog badan
Fan falanqaynta bioinffic-ka dhameystiran ee muujinta hidda-wadaha iyo qaab-dhismeedka qoraalka ah
Faa'iidooyinka adeegga
●Khibrad ballaaran: Iyada oo la raacayo diiwaan-galinta wadada ka badan 600,000 oo muunado ah oo ka jira BMKENE, oo ah noocyo muunad kala duwan ah sida dhaqamada unugyada, unugyada, iyo dareeraha jirka, kooxdeenu waxay u keentaa khibrad badan oo mashruuc kasta ah. Waxaan si guul leh u xirnaayeen in kabadan 100,000 oo mashruuc Mrna-SEQ mashruuc ka mid ah domain cilmi-baaris oo kala duwan.
●Xakamaynta tayada adag ee adag: Waxaan ka hirgelinaa qodobbada kontoroolka core ee dhammaan marxaladaha, tusaale ahaan muunadda iyo u diyaarinta maktabadda si ay u helaan isku xigxiga iyo bioinformatics. Korjoogteyntan sifiican loo kormeer ayaa hubiya gaarsiinta natiijooyin tayo sare leh oo joogto ah.
● Qoraal dhameystiran: Waxaan u isticmaalnaa xog-ururinta badan si aan uga shaqeyno hidda-wadaha dib-u-eega ee lagu muujiyey (DEG-yada) oo sameeya falanqaynta kobcinta ee u dhiganta, iyagoo siinaya aragtiyo ku saabsan geedi socodka gacanta iyo molikalar ee salka ku haya jawaabta qoraalka.
●Taageerada iibka ka dib: Balankeenna wuxuu ku fidsan yahay dhameystirka mashruuca ee leh muddo 3 bilood iibka iibka ah ka dib. Inta lagu jiro waqtigan, waxaan bixinaa dabagal dabagal mashruuc, gargaar dhibaatayn, iyo Q & SS & Kalfadhiyo si wax looga qabto wixii su'aalo ah ee la xiriira natiijooyinka.
Shuruudaha muunadda iyo dhalmada
| Maktabad | Istaraatiijiyadda isku xigta | Xogta lagu taliyay | Xakamaynta Tayada |
| Poly si fiican | Iftiimina PE150 Dnbseq-t7 | 6-10 GB | Q3t85% |
Shuruudaha muunadda:
Nucleotatides:
| CODKA. (NG / μL) | Tirada (μg) | Daahir | Sharaf |
| 10 | ≥ 0.2 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Dhirta: Rin≥4.0; Xayawaanka: Rin≥4.5; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
Dhirta:
Xidid, asliga ah ama petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
● Xayawaanka:
Wadnaha ama xiidmaha: 300 mg
Viscera ama maskaxda: 240 mg
Murqaha: 450 mg
Lafaha, timaha ama maqaarka: 1g
● Arthropods:
Cayayaanka: 6g
Crustacea: 300 mg
Dhiiga oo dhan: 1 tuubbo
● Unugyada: 106 unugyada
Keenista muunad
Weelka: 2 ml centrifouge tuubada (foil tin tin laguma talinayo)
Calaamadaha muujinta: Kooxda + waxay ku daydaa tusaale ahaan a1, A2, A3; B1, B2, B3.
Shixnad:
1. Ice-ICE: Saamiyada ayaa loo baahan yahay in lagu buuxiyo bacaha oo lagu aasay baraf qalalan.
2. Tuunnada lagu qurxiyo
Socodka shaqada adeegga
Naqshadeynta Tijaabada
Keenista muunad
Soo saarida RNA
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Bioinformatics
Golaha Transcritome iyo Xulashada Ungerite
Faahfaahinta Unigient
Sawirka muunad iyo qiimeynta buugga bayoolajiga
Hiddo-wadaha si kala soocaya ee muujiyey (DEG-yada)
Faahfaahinta Shaqada ee Shaqada
Kobcinta shaqeysa ee DEGS
Baadh horumarka ay fududeeyaan BMKGene's's Eukaryotic's Eukaryat Scraquions Scraquions ee loo maro aruurinta daaqsinta daabacaadaha.
Shen, F. et al. (2020) 'Devo Trampibriptome Gole' iyo tilmaanta hiddaha galmada ee godadka ee galmada kafiska ah (sirus asitus) ', setros, 112 (3), pp. Doi: 10.1016 / J.ygegalyo.2020.01.026.
Zhang, c. et al. (2016) 'Falanqaynta Transcriptome ee dheef-shiid kiimikaad inta lagu gudajiro bararka iyo horumarka basasha (Allium Cepa l.)', xuduudaha sayniska dhirta, 7 (Sebtember), p. 212763. DOI: 10.3389 / FPLS.2016.01425 / Bibtex.
Zhu, c. et al. (2017) 'Devo Golaha, astaamaha iyo faahfaahinta transcritomome of Sarcocheilichililiss sinenssis', plos mid, 12 (2). doi: 10.1371 / Journal.POon.Tone.0171966.
Zou, L. et al. (2021) Falanqaynta Devocport ombercriport waxay bixisaa aragtiyo ku saabsan dulqaadkii cusbada ee podocarpus macropylusulus hoostiisa bayoolajiyada dhirta ee '', bayoolajiga dhirta ee loo yaqaan '' ', bayoolajiga dhirta ee BMC, 21 (1), PP. 1-17. DI: 10.11186 / S12870-021-032-032-13274 / Tirooyin / 9.
