Taxanaha Metagenomic -NGS
Metagenome-ku waa ururinta wadarta walxaha hidde-sidaha ee bulshada isku dhafan ee noolaha, sida deegaanka iyo metagenom-yada aadanaha. Waxa uu ka kooban yahay genome-ka noolaha la beeran karo iyo kuwa aan la beeran karin labadaba. Isku-xigta metagenomic ee Shotgun ee NGS waxay awood u siinaysaa daraasadda muuqaalladan genomic-ga ah ee ku dhex-jira muunadaha deegaanka iyadoo la siinayo wax ka badan sifada taxonomic, iyadoo sidoo kale siinaya aragtiyo qaawan oo ku saabsan kala duwanaanshaha noocyada, dhaqdhaqaaqyada fara badan, iyo qaabdhismeedka dadweynaha ee kakan. Marka laga soo tago daraasaadka taxonomiga, metagenomics shotgun waxa kale oo ay bixisaa aragti genomics shaqaynaysa, taas oo awood u siinaysa sahaminta hiddo-sidaha la calaamadeeyay iyo doorkooda wax-ka-qabashada ee hababka deegaanka. Ugu dambayntii, samaynta isku xidhka isku xidhka walxaha hidde-sidaha iyo arrimaha deegaanka waxa ay gacan ka geysataa faham buuxa ee isdhexgalka adag ee ka dhexeeya bulshooyinka microbial iyo asalkooda deegaan. Gebagebadii, isku xigxiga metagenomic waxa uu u taagan yahay sidii qalab muhiim u ah daah-furka dhexgalka genomic ee bulshooyinka yaryar ee kala duwan, isaga oo iftiiminaya xidhiidhada dhinacyo badan leh ee ka dhexeeya hidde-sideyaasha iyo deegaanka ee ka jira hab-nololeedyadan kakan.
Platforms: Illumina NovaSeq iyo DNBSEQ-T7
Faa'iidooyinka Adeegga
●Go'doominta iyo Habka Beerista Xorta ah ee Xog-ururinta Bulshada MicrobialAwoodsiinta isku xigxiga walxaha hidde-sideyaasha noolaha aan la beeran karin.
●Qaraarka Sare: Soo ogow noocyada tirada yar ee shaybaarada deegaanka.
●Falanqaynta Bioinformatics oo dhamaystiran:Aan diirada saarnayn oo kaliya kala duwanaanshaha cashuuraha laakiin sidoo kale kala duwanaanshaha shaqada ee bulshada.
●Khibrad Balaadhan:Iyada oo la raacayo rikoorka ah in si guul leh loo xiro mashruucyo badan oo metagenomics ah oo laga sameeyay qaybo cilmi baaris oo kala duwan iyo habaynta in ka badan 200,000 muunado, kooxdeenu waxay u keentaa khibrad badan mashruuc kasta.
Tilmaamaha Adeegga
| Madal isku xigxiga | Istaraatiijiyada Taxanaha | Xogta lagu taliyay | Xakamaynta tayada |
| Illumina NovaSeq ama DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Shuruudaha Adeegga
| Xooga saarida (ng/µL) | Tirada guud (ng) | Mugga (µL) |
| ≥1 | ≥30 | ≥20 |
● Ciidda / dhoobada: 2-3g
● Waxyaalaha xiidmaha-xoolaha: 0.5-2g
● Waxa ku jira xiidmaha- cayayaan: 0.1-0.25g
● Dusha dhirta (barar la xoojiyey): 0.5-1g
● maraq qashin ah oo la hodmay: 0.2-0.5g
● Saxarada (xoolaha waaweyn): 0.5-2g
● Saxarada (jiirka): 3-5 hadhuudh
● Dheecaanka sambabada alveolar: warqad filter
● Xuubka siilka: 5-6 suuf
● Maqaarka/maqaarka xubinta taranka/candhuufta/xuubka jilicsan ee afka
● Ilmo-nooleyaasha dusha sare: 5-6 suuf
● Waterbody/hawo/filim noole: warqad filter
● Endophytes: 2-3g
● Plaque-ga Ilkaha: 0.5-1g
Socodka Shaqada Adeegga
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Waxa ku jira falanqaynta soo socota:
● Isku dayga ilaalinta tayada xogta
● Isku-ururinta Metagenome iyo saadaalinta hidda-wadaha
● Tilmaamaha hidda-wadaha
● Taxonomic alfa falanqaynta kala duwanaanshaha
● Falanqaynta shaqada ee bulshada: shaqada nafleyda, dheef-shiid kiimikaadka, iska caabbinta antibiyootiga
● Falanqaynta kala duwanaanta shaqada iyo taxonomiga labadaba:
Falanqaynta kala duwanaanta beta
Falanqaynta kooxaha dhexdooda
Falanqaynta isku xidhka: inta u dhaxaysa arrimaha deegaanka iyo ka kooban OUT iyo kala duwanaanta
Falanqaynta shaqada: iska caabbinta antibiyootiga KAARD
Falanqaynta kala duwan ee waddooyinka dheef-shiid kiimikaadka ee KEGG: khariidadda kulaylka ee waddooyinka muhiimka ah
Kala duwanaanshaha Alpha ee qaybinta cashuuraha: ACE index
Kala duwanaanshaha Beta ee qaybinta cashuuraha: PCoA
Sahami horumarka ay fududeeyeen adeegyada isku xigxiga ee metagenome ee BMKGene ee Illumina iyada oo loo marayo ururinta daabacaado la soo xulay.
Hai, Q. iyo al. (2023) 'Fayraska dheef-shiid kiimikaadka iyo dheef-shiid kiimikaadka ee isbeddelada ku jira xiidmaha mindhicirka ee tufaax qaanso-roobaadka ( Oncorhynchus mykiss) oo uu ku dhacay fayraska necrosis hematopoietic necrosis ee heerkulka biyaha dhaqamada kala duwan',Xuduudaha Microbiology, 14, p. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. iyo al. (2023) "Bulshooyinka microbial, hiddo-wadaha iska caabinta, iyo khatarta iska caabinta ee harooyinka magaalooyinka ee gobollada kala duwan ee trophic: Xiriirinta gudaha iyo saameynta dibadda',Joornaalka Horumarka Agabka Khatarta ah, 9, p. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. iyo al. (2022) 'Falanqaynta dheef-shiid kiimikaad ayaa daaha ka qaaday faraqa u dhexeeya curinta iyo shaqada udhaxaysa noolaha dareeraha ah iyo kuwa adag ee la xidhiidha nooleyaasha adhiga',Xuduudaha Microbiology, 13, p. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. iyo al. (2023) 'Mikrobiota ka soo jeeda doofaarka Ningxiang cayilan waxay dib u soo celisaa dheef-shiid kiimikaadka carnitine si kor loogu qaado kaydinta acid dufanka muruqa ee doofaarka DLY ee caatada ah',Hal-abuurka, 4(5), b. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. iyo al. (2023) 'Fikirka metagenomic ee khataraha iman kara ee wakiilka bio/ balaastikada aan dumin karin iyo qashinka aan caaga ahayn ee meelaha sare iyo hoose ee Haihe Estuary, Shiinaha',Sayniska Guud ee Deegaanka, 887, p. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
