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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing

PacBio-Full-length 16S/18S/ITS Amplicon Sequencing Umfanekiso ofakiweyo

Iqonga le-Amplicon (16S / 18S / ITS) liphuhliswa kunye neminyaka yamava kuhlalutyo lweprojekthi ye-microbial diversity, equlethe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial, umxholo wokuhlalutya ucebile kwaye ubanzi, kunye neziphumo zohlalutyo zinikezelwe ngohlobo lweengxelo zeprojekthi;Umxholo wohlalutyo lobuqu uhlukile.Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu.Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Ukusebenzisa idatha yakho

Ukuhamba komsebenzi weBioinformatics

Umngcelele wedatha ekrwada

Ubude obupheleleyo non-chimeric(FLNC) ifundeka ukuchongwa

Ukuchongwa kwemibhalo yemvumelwano

Ukuchongwa okubhaliweyo

Ukwakhiwa kwe-unigenes

Ukuchongwa kwe-isoform

Enye indlela yokudibanisa

Ukudityaniswa kolunye uhlobo

Uhlalutyo lwe-APA

I-APA eyahlukileyo

Imfuza yeFusion

Ijini yokudibanisa eyahlukileyo

Iimfuza ze-Homologuos

Ukulungelelaniswa kolwakhiwo lwemfuza

Uhlalutyo lwe-SNP

Uqikelelo lwe-lncRNA

Ingqikelelo ekujoliswe kuyo ye-lncRNA

Uqikelelo lweCDS

Into yokukhutshelwa

Ubungakanani benkcazo

Uhlalutyo lwentetho eyohlukileyo (kwimizila yemfuza nakwimibhalo ebhaliweyo)

Inkcazo esebenzayo

Ukutyebisa okusebenzayo

Inethiwekhi yeprotheyini yokunxibelelana

Ngaphambili: I-PacBio-ubude obugcweleyo be-Transcriptome (Non-Reference)

Okulandelayo: IBacterial kunye neFungal Whole Genome Re-Sequencing

I-CircRNA-ulandelelwano-uhlalutyo-lokuhamba komsebenzi

Bioinformatics

I-CircRNA-ulandelelwano-uhlalutyo-lokuhamba komsebenzi

fumana isicatshulwa

Bhala umyalezo wakho apha kwaye uwuthumele kuthi

Imvelisoiindidi

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