Ixesha elide Non-coding Ulandelelwano-Illumina
Iinkonzo eziluncedo
●Uhlalutyo oludibeneyo lwe-mRNA kunye ne-lncRNA: ngokudibanisa ubungakanani be-mRNA transcripts kunye nesifundo se-lncRNA kunye neethagethi zabo, kunokwenzeka ukuba ufumane inkcazo enzulu yendlela yokulawula ephantsi kwempendulo yeselula.
●UbuGcisa obuBanzi: Iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye, kunye nerekhodi yokusetyenzwa ngaphezulu kwe-23,000 yeesampulu kwi-BMK ethatha iintlobo ngeentlobo zeesampulu kunye neeprojekthi ze-lncRNA.
●Ulawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
●Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Iqonga | Idatha ecetyiswayo | Idatha ye-QC |
| Ithala leencwadi le-rRNA liphelile | Illumina PE150 | 10-16 Gb | Q30≥85% |
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
● Izityalo:
Ingcambu, Isiqu okanye iPetal: 450 mg
Igqabi okanye Imbewu: 300 mg
Isiqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye Amathumbu: 450 mg
Viscera okanye Brain: 240 mg
Izihlunu: 600 mg
Amathambo, Iinwele okanye Ulusu: 1.5g
● IiArthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi elipheleleyo:2 iityhubhu
● Iiseli: 106 iiseli
● ISerum kunye nePlasmayena: 6 ml
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3; B1, B2, B3.
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu ze-RNAstable: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
Uhlalutyo oluhlukeneyo lweGene Expression (DEGs).
Ubungakanani benkcazo ye-lncRNA - ukudibanisa
Ukutyebisa kwi-lncRNA yofuzo ekujoliswe kuyo
Uhlahlelo lwe-Joint mRNA kunye ne-lncRNA yendawo - i-Circos plot (isangqa esiphakathi yi-mRNA kunye ne-cicrlce yangaphakathi yi-lncRNA)
Phonononga inkqubela phambili eququzelelwa yi-BMKGene's lncRNA iinkonzo zokulinganisa ngengqokelela egciniweyo yopapasho.
Ji, H. et al. (2020) 'Ukuchonga, ukubikezela okusebenzayo, kunye nokuqinisekiswa okungundoqo kwe-lncRNA ye-lncRNAs ezinxulumene noxinzelelo olubandayo kwisibindi segundane', IiNgxelo zeNzululwazi 2020 10: 1, 10 (1), iphepha 1-14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'Uhlalutyo oludibeneyo lwe-Transcriptomic lutyhila i-Immune Mechanism ye-CyHV-3-Resistant Common Carp Strain', i-Frontiers kwi-Immunology, i-12, iphe. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ et al. (2022) 'I-Multi-Omics Integration-Based Prioritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Iimpawu zeMolekyuli kunye naBagqatswa beziyobisi', iFrontiers kwi-Oncology, i-12, iphe. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'I-Genetic dissection ye-gene coexpression network ephantsi kwe-photosynthesis kwi-Populus', i-Plant Biotechnology Journal, i-18 (4), iphe. 1015-1026. doi: 10.1111/PBI.13270.
UZheng, H. et al. (2022) 'I-Global Regulatory Network ye-Dysregulated Gene Expression kunye neSibonakaliso esingaqhelekanga seMetabolic kwiiSeli ze-Immune kwi-Microenvironment ye-Graves' Disease kunye ne-Hashimoto's Thyroiditis', iMida kwi-Immunology, i-13, iphe. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
