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I-nucleus ye-RNA enye
Ukuqhubela phambili ekuthathweni kweseli enye kunye nobuchule bokwakha ithala leencwadi ngalinye kudityaniswe nolandelelwano olugqithileyo luvumela izifundo zokuchazwa kofuzo ngokwesiseko seseli-nge-seli.Ivumela uhlalutyo olunzulu kunye olupheleleyo lwenkqubo kwiiseli ezintsonkothileyo, apho ubukhulu becala inqanda ukufihla ukungafani kwazo ngokuthatha umndilili wazo zonke iiseli.
Nangona kunjalo, ezinye iiseli azifanelekanga ukuba zenziwe zibe yi-single-cell suspension, ngoko ke ezinye iindlela zokulungiselela iisampulu-i-nucleus extraction evela kwizicubu ziyafuneka, oko kukuthi, i-nucleus ikhutshwa ngokuthe ngqo kwiithishu okanye kwiseli kwaye ilungiswe ibe yi-single-nucleus suspension for single- ulandelelwano lweseli.
I-BMK inikezela nge-10 × i-Genomics yeChromium TM esekelwe kwinkonzo yokulandelelana kwe-RNA yeseli enye.Le nkonzo isetyenziswe ngokubanzi kwizifundo kwizifundo ezinxulumene nesifo, ezifana nokwahlukana kweeseli zomzimba, i-tumor heterogeneity, uphuhliso lwezicubu, njl.
I-Spatial transcriptome chip: 10× Genomics
Iqonga: I-Illumina NovaSeq Platform
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I-BMKMANU S1000 i-Spatial Transcriptome
I-Spatial transcriptomics imi phambili kubuchule obutsha bezenzululwazi, ixhobisa abaphandi ukuba baphengulule kwiipateni ezintsonkothileyo zokubonakaliswa kofuzo ngaphakathi kwezicubu ngelixa begcina imeko yabo yendawo.Phakathi kwamaqonga ahlukeneyo, iBMKGene iphuhlise iBMKManu S1000 Spatial Transcriptome Chip, iqhayisaisisombululo esongeziweyoye 5µM, ifikelela kuluhlu olusezantsi, kunye nokwenzauseto lwesisombululo esinamanqanaba amaninzi.Itshiphu ye-S1000, enamabala amalunga nezigidi ezi-2, isebenzisa ii-microwells ezalekwe ngamaso alayishwe nge-spatially barcoded capture probes.Ilayibrari ye-cDNA, etyetyiswe ngeebhakhowudi zendawo, ilungiswa ukusuka kwitshiphu ye-S1000 kwaye emva koko ilandelelaniswe kwiqonga le-Illumina NovaSeq.Ukudityaniswa kweesampulu ezinebhakhowudi yendawo kunye nee-UMIs ziqinisekisa ukuchaneka kunye nokuchaneka kwedatha eyenziweyo.Uphawu olukhethekileyo lwe-chip ye-BMKManu S1000 ilele kukuguquguquka kwayo, inika useto lwesisombululo esinamanqanaba amaninzi anokuthi alungelelaniswe kakuhle kwiithishu ezahlukeneyo kunye namanqanaba eenkcukacha.Oku kuguquguquka kubeka itshiphu njengokhetho olubalaseleyo lwezifundo ezahlukeneyo zoshicilelo lwesithuba, ukuqinisekisa ukudityaniswa kwendawo echanekileyo kunye nengxolo encinci.
Ukusebenzisa i-chip ye-BMKManu S1000 kunye nobunye ubuchwephesha be-transcriptomics yendawo, abaphandi banokufumana ukuqonda okungcono kwentlangano yendawo yeeseli kunye nokunxibelelana kweemolekyuli ezintsonkothileyo ezenzeka ngaphakathi kwezicubu, zibonelela ngolwazi oluxabisekileyo kwiinkqubo eziphantsi kwenkqubo yebhayoloji kuluhlu olubanzi lweenkalo, kubandakanya. i-oncology, i-neuroscience, i-biology yophuhliso, i-immunology kunye nezifundo zebhotani.
Iqonga: BMKManu S1000 chip kunye Illumina NovaSeq
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I-10x ye-Genomics yeVisium ye-Spatial Transcriptome
I-Spatial transcriptomics yitekhnoloji enqamlezileyo evumela abaphandi ukuba baphande iipatheni zokubonakaliswa kofuzo ngaphakathi kwezicubu ngelixa begcina imeko yabo yendawo.Iqonga elinye elinamandla kule domain yi-10x Genomics Visium edityaniswa nokulandelelana kwe-Illumina.Umgaqo we-10X Visium ulele kwi-chip ekhethekileyo enendawo ekhethiweyo yokubamba apho amacandelo e-tissue abekwe khona.Le ndawo yokubamba iqulethe iindawo ezinebhakhowudi, nganye ihambelana nendawo eyodwa ngaphakathi kwethishu.Iimolekyuli ze-RNA ezibanjiweyo kwiithishu zibhalwa emva koko zibhalwe nge-molecular identifiers (UMIs) ezikhethekileyo ngexesha lenkqubo yokubhala umva.Ezi ndawo zinebhakhowudi kunye nee-UMIs zenza imephu yendawo echanekileyo kunye nobungakanani bokubonakaliswa kofuzo kwisisombululo seseli enye.Ukudityaniswa kweesampulu ezinebhakhowudi yendawo kunye nee-UMIs ziqinisekisa ukuchaneka kunye nokuchaneka kwedatha eyenziweyo.Ngokusebenzisa le teknoloji ye-Spatial Transcriptomics, abaphandi banokufumana ukuqonda okunzulu malunga nentlangano yendawo yeeseli kunye nentsebenziswano eyinkimbinkimbi ye-molecular eyenzeka ngaphakathi kwezicubu, inika ulwazi oluxabisekileyo kwiindlela eziphantsi kweenkqubo zebhayoloji kwiinkalo ezininzi, kuquka i-oncology, neuroscience, i-biology yophuhliso, i-immunology. , kunye nezifundo zebhotani.
Iqonga: I-10X Genomics Visium kunye ne-Illumina NovaSeq
