Ulandelelwano lweMetagenomic -NGS
I-metagenome yingqokelela yezinto ezipheleleyo zemfuza yoluntu oluxubileyo lwezinto eziphilayo, ezifana neemetagenome zendalo kunye nezomntu. Iqulethe iigenome zeentsholongwane ezinokulinywa kunye nezingenakulinywa. Ukulandelelana kwe-metagenomic ye-Shotgun nge-NGS kwenza kube lula ukufunda ezi ndawo zintsonkothileyo ze-genomic ezifakwe kwiisampuli zendalo ngokubonelela ngaphezu kokubhala i-taxonomic, okunika nokuqonda okubanzi malunga nokwahluka kweentlobo, i-dynamics yobuninzi, kunye nezakhiwo ezintsonkothileyo zabantu. Ngaphaya kwezifundo ze-taxonomic, i-shotgun metagenomics ikwabonelela ngembono esebenzayo ye-genomics, evumela ukuphononongwa kwee-genes ezifakwe kwi-encoded kunye neendima zazo ezichazayo kwiinkqubo zendalo. Okokugqibela, ukusekwa kothungelwano lolwalamano phakathi kwezinto zemfuza kunye nezinto zendalo kunceda ekuqondeni ngokupheleleyo ukusebenzisana okuntsonkothileyo phakathi kweendawo ezintsonkothileyo kunye nemvelaphi yazo yendalo. Ukuqukumbela, ulandelelwano lwe-metagenomic lumi njengesixhobo esibalulekileyo sokutyhila ubunzima be-genomic beendawo ezahlukeneyo ezintsonkothileyo, ukukhanyisa ubudlelwane obunemiba emininzi phakathi kwe-genetics kunye ne-ecology ngaphakathi kwezi ndawo zintsonkothileyo zendalo.
Amaqonga: Illumina NovaSeq kunye ne-DNBSEQ-T7
Iingenelo zeNkonzo
●Indlela yokuzahlula kunye neyokulima engenazo iindlela zokuchonga iintsholongwane kuluntu: Ukuvumela ukulandelelaniswa kwezinto zemfuza kwizinto eziphilayo ezingalinywayo.
●Isisombululo Esiphezulu: Fumana iintlobo zezilwanyana ezingenazo iziqhamo ezininzi kwiisampulu zokusingqongileyo.
●Uhlalutyo olupheleleyo lwe-Bioinformatics:Ayigxilanga kuphela kulwahlulo lwe-taxonomic kodwa nakwiyantlukwano yokusebenza koluntu.
●Amava amaninzi:Ngerekhodi lokuvala ngempumelelo iiprojekthi ezininzi ze-metagenomics kwiindawo ezahlukeneyo zophando kunye nokucubungula iisampulu ezingaphezu kwama-200,000, iqela lethu lizisa amava amaninzi kwiprojekthi nganye.
Iinkcukacha zeNkonzo
| Iqonga lokulandelelanisa | Icebo Lokulandelelanisa | Idatha iyacetyiswa | Ulawulo lwemeko |
| I-Illumina NovaSeq okanye i-DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Iimfuno zeNkonzo
| Uxinzelelo (ng/µL) | Isixa-mali sisonke (ng) | Umthamo (µL) |
| ≥1 | ≥30 | ≥20 |
● Umhlaba/udaka: 2-3g
● Umthamo wamathumbu - isilwanyana: 0.5-2g
● Umxholo wamathumbu - isinambuzane: 0.1-0.25g
● Umphezulu wesityalo (inkunkuma etyebileyo): 0.5-1g
● Umhluzi wokubilisa otyebileyo intlenga): 0.2-0.5g
● Indle (izilwanyana ezinkulu): 0.5-2g
● Indle (igundane): 3-5 iinkozo
● Ulwelo lokuhlamba i-alveolar yemiphunga: iphepha lesihluzi
● I-swab yesisu: ii-swab ezi-5-6
● I-swab yolusu/yesini/amathe/izicubu ezithambileyo zomlomo/i-pharyngeal swab/i-rectal swab: ii-swab ezi-2-3
● I-microorganism engaphezulu: iiswabhu ezi-5-6
● Umzimba wamanzi/umoya/i-biofilm: iphepha lokucoca
● Ii-Endophytes: 2-3g
● Iplaque yamazinyo: 0.5-1g
Ukuhamba komsebenzi wenkonzo
Ukuhanjiswa kwesampulu
Ukwakhiwa kweThala leeNcwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zasemva kokuthengisa
Kubandakanya uhlalutyo olulandelayo:
● Ukulandelelanisa ulawulo lomgangatho wedatha
● Ukuhlanganiswa kwe-Metagenome kunye nokuqikelela i-gene
● Inkcazelo yejini
● Uhlalutyo lokwahlukahlukana kwe-alpha ye-taxonomic
● Uhlalutyo olusebenzayo loluntu: umsebenzi webhayoloji, imetabolism, ukumelana neentsholongwane
● Uhlalutyo malunga nokwahlukahlukana kokusebenza kunye nokwe-taxonomic:
Uhlalutyo lokwahlukahluka kwe-Beta
Uhlalutyo phakathi kwamaqela
Uhlalutyo lolwalamano: phakathi kwezinto ezingqongileyo kunye nokwakheka kunye nokwahlukahluka kwe-OUT
Uhlalutyo olusebenzayo: Ukumelana ne-CARD antibiotic
Uhlalutyo olwahlukileyo lweendlela ze-metabolic ze-KEGG: imephu yobushushu yeendlela ezibalulekileyo
Ukwahlukahluka kwe-alpha kokusasazwa kwe-taxonomic: i-ACE index
Ukwahlukahluka kwe-Beta kokusasazwa kwe-taxonomic: PCoA
Hlola inkqubela phambili eququzelelwe ziinkonzo ze-BMKGene zokulandelelanisa i-metagenome kunye ne-Illumina ngokusebenzisa ingqokelela yeempapasho ezikhethiweyo.
UHai, Q. et al. (2023) 'Uhlalutyo lwe-metagenomic kunye ne-metabolomic lotshintsho kumxholo wamathumbu we-rainbow trout (Oncorhynchus mykiss) osulelwe yintsholongwane ye-hematopoietic necrosis esulelayo kumaqondo obushushu ahlukeneyo okukhula kwamanzi',Imida kwiMicrobiology, 14, iphe. 1275649. doi: 10.3389/FMICB.2023.1275649.
UMao, C. et al. (2023) 'Uluntu lweentsholongwane, iijini zokumelana, kunye neengozi zokuchasana kumachibi asezidolophini ahlukeneyo: Amakhonkco angaphakathi kunye neempembelelo zangaphandle',Ijenali yoPhuhliso lwezinto eziyingozi, 9, iphe. 100233. doi: 10.1016/J.HAZADV.2023.100233.
USu, M. et al. (2022) 'Uhlalutyo lweMetagenomic lutyhile umahluko kwisakhiwo kunye nomsebenzi phakathi kweentsholongwane ezidityaniswe nolwelo kunye neziqinileyo zeSheep Rumen',Imida kwiMicrobiology, 13, iphe. 851567. doi: 10.3389/FMICB.2022.851567.
UYin, J. et al. (2023) 'I-microbiota ephuma kwihagu i-Ningxiang etyebileyo iphinda ifake i-carnitine metabolism ukukhuthaza ukufakwa kwe-muscle fatty acid kwiihagu ezithambileyo ze-DLY',Uyilo olutsha, 4(5), iphe. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Iingcamango zeMetagenomic malunga neengozi ezinokubakho zeplastiki emele i-bio/non-degradable kunye nenkunkuma engeyiyo iplastiki kwiindawo eziphezulu nezisezantsi zeHaihe Estuary, eTshayina',Isayensi yeNdalo ePheleleyo, 887, iphe. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
