I-meagemic ye-meagemic -ngs
I-metagenome yingqokelela yezinto ezikhoyo zofuzo zoluntu oluhlanganisiweyo lwezinto eziphilayo, ezifana nendawo okusingqongileyo kunye ne-mekelomes yomntu. Iqulethe ii-genomes zoomabini omabini kunye nokungafumaneki iintsholongwane. I-Shorgun Meeagemic ukulandelelana kwe-NGS yenza ukuba ukufundisisa kwale mimandla yokusingqongileyo ngokubonelela ngaphezulu kwe-proilling iyantlukwano iyahluka, ikwanguwa kwezahlulo zentabalala, kunye nezakhiwo ezinzima. Ngaphandle kwezifundo ze-teknolomic, i-Shoomegraphon ikwabonelela ngendlela efanelekileyo yokujonga i-iqulomics, ivumele ukuhlola ii-genes zemveliso enxibe iinkqubo zendalo. Okokugqibela, ukusekwa kweenethiwekhi zonxibelelwano phakathi kwezinto zemfuza kunye nezinto zokusingqongileyo zinegalelo ekuqondeni okugqwesileyo kwe-indericate phakathi koluntu oluntsonkothileyo kunye nemvelaphi yendalo. Ukuqukumbela, ukulandelelana kwe-meagenocic kumi njengesixhobo esibalulekileyo sokutyhila intshukumo ye-genomic yoluntu oluyahlukeneyo, lukhanyisa ubudlelwane obuninzi obuninzi phakathi kwezinto zendalo zendalo.
Iqonga: I-Indlorina Novaq kunye ne-DNBSEQ-T7
Izibonelelo zeNkonzo
●Ukuhlukaniswa kunye nokuhlakulela indlela yokutyala ye-microbial booluntu: Ukwenza ukubakho okulandelelana kwezinto zemvelo kwizinto eziphilayo.
●Isisombululo esiphezulu: Fumana iindidi zentabalala ephantsi kwiisampulu zokusingqongileyo.
●Uhlalutyo lwe-bioinformaticsUkugxila kungekuphela nje kwi-upernolomic kuphela kodwa nakwiyantlukwano esebenzayo yoluntu.
●Amava abalulekileyo:Ngerekhodi ye-track yokuvala ngempumelelo iiprojekthi zeMeetnomids ezininzi kwi-Domanies ezahlukeneyo zophando kunye neesampulu ezingaphezu kwe-200,000, iqela lethu lizisa ubutyebi bamava kuzo zonke iiprojekthi.
Inkcazo yenkonzo
| Iqonga lokugcwala | Isicwangciso sokulandelelana | Idatha eyacetyiswayo | Ulawulo lwemeko |
| I-Ilimina Novayq okanye i-DNBSEQ-T7 | Pe150 | I-6-20GB | I-Q30≥85% |
Iimfuneko zeNkonzo
| Uxinzelelo (nge / μl) | Inani lilonke (NG) | Umthamo (μl) |
| ≥1 | ≥30 | ≥20 |
● Umhlaba / i-sludge: 2-3g
● Umxholo wemixholo-isilwanyana: 0.5-20
● Iziqulatho zamachweba: 0.1-0.25g
● Umphezulu weSityalo (i-sedimed): 0.5-1g
● Ukubiyela i-bhrication ye-sediment ye-sediments): 0.2-0.5g
● I-faeces (izilwanyana ezinkulu): 0.5-20
● I-faeves (imouse): Ii-3s-5erins
● I-pulmonary alveolar fluid fluid: Iphepha lokucoca
● I-Vaginal Swab: 5-6 swabs
● Ulusu / iShablional Swab / I-Salvain ethambileyo
● I-microorganism yomphezulu: i-5-6 swabs
● Umanzi wamanzi / umoya / i-biofilm: iphepha lokucoca
● I-Endophytes: 2-3g
● I-Place yamazinyo: 0.5-1g
Ukuhamba kwenkonzo
Ukuhanjiswa kwesampula
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
Kubandakanya nohlalutyo olulandelayo:
● Ukulandelelanisa umgangatho wedatha
● Indibano ye-metagenome kunye ne-gene uqikelelo
● Isichazi-magama
● Uhlalutyo lwe-Alfomic Uhlalutyo lohlalutyo
● Uhlalutyo olusebenzayo loluntu: Umsebenzi webhayoloji, i-metabolic, ukuchasana ne-antibiotic
● Uhlalutyo malunga nokusebenza kokubini kunye neyantlukwano yeteknoloji:
Uhlalutyo lwe-Beta
Uhlalutyo lweqela
Uhlalutyo lokuCwangciswa: phakathi kwezinto zokusingqongileyo kunye nokuqulunqwa kunye neyantlukwano
Uhlalutyo lokuSebenza: Ikhadi le-antibiotic
Uhlalutyo lwe-kegg ye-kegg metabolic indlela: i-affmap yeendlela ezibalulekileyo
Ukwahlukahlukaniso kwe-alpha
I-Beta iyantlukwano yokuhanjiswa kwetekisi: i-PCOA
Jonga i-veractions eququzeleleke yinkonzo yokuhlawula i-BMKgene
Hai, q. et al. .Imida kwi-microbiology, 14, iphe. 1275649. Doi: 10.3389 / FEMB.2023.127549.
Mao, c. et al. .Ijenali yejenali yezixhobo ezinobungozi, 9, iphe. I-100233. Doi: 10.1016 / j.hazadv.2023.100233.
Su, m. et al. .Imida kwi-microbiology, 13, p. 851567. Doi: 10.3389 / FEMB.2022.851567.
Yin, j. et al. .Ubuchule, 4 (5), iphe. I-100486. Doi: 10.1016 / J.xinn.2023.100486.
Zhao, x. et al. .Inzululwazi yendalo iyonke, 887, iphe. 164026. Doi: 10.1016 / J.Scitoten.2023.164026.
