Ulandelelwano lweMetagenomic -NGS
I-metagenome yingqokelela yemathiriyeli yemfuzo iyonke yoluntu oluxubeneyo lwezinto eziphilayo, ezifana nokusingqongileyo kunye neemetagenomes zabantu. Iqulethe ii-genomes zombini ii-microorganisms ezinokulinywa kunye nezingenakulinywa. I-Shotgun metagenomic sequencing kunye ne-NGS yenza ukuba kuphononongwe le mihlaba intsonkothileyo ye-genomic emiliselwe kwiisampulu zokusingqongileyo ngokubonelela ngokungaphaya kweprofayili ye-taxonomic, inika ulwazi oluphangaleleyo kwiyantlukwano yeentlobo, ukuguquguquka kobuninzi, kunye nezakhiwo ezintsonkothileyo zabemi. Ngaphandle kwezifundo ze-taxonomic, i-shotgun metagenomics ikwabonelela ngembono esebenzayo ye-genomics, eyenza ukuphononongwa kofuzo olufakwe kwikhowudi kunye neendima zabo zokubeka kwiinkqubo zendalo. Okokugqibela, ukusekwa kothungelwano lothungelwano phakathi kwezinto zofuzo kunye nemiba yokusingqongileyo inegalelo ekuqondeni okupheleleyo konxibelelwano oluntsonkothileyo phakathi koluntu lwemicrobial kunye nemvelaphi yazo yendalo. Ukuqukumbela, ulandelelwano lwe-metagenomic limi njengesixhobo esibalulekileyo sokutyhila ubugocigoci be-genomic boluntu olwahlukeneyo lwe-microbial, ikhanyisela ubudlelwane obuninzi phakathi kofuzo kunye ne-ikholoji ngaphakathi kwezi zixokelelwano zendalo ezintsonkothileyo.
Iiplatforms: Illumina NovaSeq kunye ne-DNBSEQ-T7
Iinkonzo eziluncedo
●Ukwahlulwa kunye neNdlela yokuLima-eMahala yeProfayili yoLuntu lweMicrobial: Ukwenza ulandelelwano lwemathiriyeli yemfuzo kwizinto eziphilayo ezingalinywanga.
●Isisombululo esiphezulu: Khangela iintlobo eziphantsi kobuninzi kwiisampulu zokusingqongileyo.
●Uhlalutyo oluBanzi lweBioinformatics:Ayijoliswanga kuphela kwiiyantlukwano zetaxonomic kodwa nakwiyantlukwano yokusebenza koluntu.
●Amava Abanzi:Ngerekhodi lokuvala ngempumelelo iiprojekthi ezininzi ze-metagenomics kwiindawo ezahlukeneyo zophando kunye nokusetyenzwa kweesampuli ze-200,000, iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye.
IiNgcaciso zeNkonzo
| Iqonga lokulandelelana | Isicwangciso solandelelwano | Idatha iyacetyiswa | Ulawulo lwemeko |
| Illumina NovaSeq okanye DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
IiMfuno zeNkonzo
| Ugxininiso (ng/µL) | Iyonke imali (ng) | Umthamo (µL) |
| ≥1 | ≥30 | ≥20 |
● Umhlaba / udaka: 2-3g
● Umxholo wamathumbu-isilwanyana: 0.5-2g
● Iziqulatho zamathumbu-insect: 0.1-0.25g
● Umphezulu wezityalo (intlenga etyetyisiweyo): 0.5-1g
● Umhluzi wokubilisa utyetyiswe intlenga): 0.2-0.5g
● Ilindle (izilwanyana ezikhulu): 0.5-2g
● Indle (imouse): 3-5grains
● I-pulmonary alveolar lavage fluid: iphepha lokucoca
● I-swab ye-vaginal: i-5-6 swabs
● Ulusu/iswabhu yelungu lobuni/amathe/isihlunu esithambileyo somlomo/iswab yepharyngeal/i-rectal swab: 2-3 swabs
● Surface microorganism: 5-6 swabs
● Umzimba wamanzi/umoya/i-biofilm: iphepha lokucoca
● I-Endophytes: 2-3g
● I-Dental Plaque: 0.5-1g
Ukuhamba komsebenzi weNkonzo
Ukuhanjiswa kwesampuli
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Ibandakanya uhlalutyo olulandelayo:
● Ukulandelelanisa ulawulo lomgangatho wedatha
● Indibano ye-Metagenome kunye nokuqikelelwa kofuzo
● Inkcazo yemfuza
● Uhlalutyo lweyantlukwano yealpha yeTaxonomic
● Uhlalutyo olusebenzayo loluntu: umsebenzi webhayoloji, i-metabolic, ukumelana ne-antibiotic
● Ucazululo kwiiyantlukwano ezisebenzayo nezetaxonomic:
Uhlalutyo lokwahluka kweBeta
Uhlalutyo lwamaqela
Uhlalutyo lonxulumano: phakathi kwemiba yokusingqongileyo kunye nokwakheka kwe-OUT kunye neyantlukwano
Uhlalutyo olusebenzayo: I-CARD yokumelana ne-antibiotic
Uhlalutyo olwahlukileyo lweendlela ze-metabolic ze-KEGG: imephu yobushushu beendlela ezibalulekileyo
Ukwahluka kwe-Alpha yokusasazwa kwe-taxonomic: isalathisi se-ACE
Ukwahluka kweBeta yokusasazwa kwetaxonomic: PCoA
Phonononga inkqubela phambili eququzelelwa yi-BMKGene ye-metagenome yolandelelwano lweenkonzo kunye ne-Illumina ngengqokelela egciniweyo yopapasho.
Hayi, Q. et al. (2023) 'Uhlalutyo lwe-Metagenomic kunye ne-metabolomic yeenguqu kwimixholo yamathumbu e-rainbow trout (Oncorhynchus mykiss) esulelwe yintsholongwane ye-hematopoietic necrosis echaphazelekayo kwiinkcubeko ezahlukeneyo zamaqondo obushushu bamanzi',Imida kwiMicrobiology, 14, iphe. 1275649. doi: 10.3389/FMICB.2023.1275649.
UMao, uC. et al. (2023) 'Uluntu lweMicrobial, iijini zokumelana, kunye nemingcipheko exhathisayo kumachibi asezidolophini akwiindawo ezahlukeneyo zetrophic: Amakhonkco angaphakathi kunye neempembelelo zangaphandle',Ijenali yeZizinto eziNcedayo eziPhambili, 9, iphe. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Uhlahlelo lweMetagenomic luDize iiyantlukwano kuKubunjwa kunye noMsebenzi phakathi kweMicroorganisms eNxibelene nolwelwe kunye neSolid-eManyaniswa neegusha zeRumen',Imida kwiMicrobiology, 13, iphe. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'I-Obese Ningxiang-derived pig-derived microbiota ibuyisela i-carnitine metabolism ukukhuthaza ukubekwa kwe-muscle fatty acid kwiihagu ezibhityileyo ze-DLY',I-Innovation, 4(5), iphe. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Ukuqonda kweMetagenomic kwimingcipheko enokubakho yommeli we-bio / iplastiki engonakaliyo kunye nenkunkuma engeyiyo iplastiki kwindawo ephezulu nasezantsi ye-Haihe Estuary, e-China',Inzululwazi yoBume beNdawo iyonke, 887, iphe. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
