Iimveliso

I-nucleus ye-RNA enye

I-nucleus enye ye-RNA Ukulandelelana koMfanekiso obonakalayo

Ukuqhubela phambili ekuthathweni kweseli enye kunye nobuchule bokwakha ithala leencwadi ngalinye kudityaniswe nolandelelwano olugqithileyo luvumela izifundo zokuchazwa kofuzo ngokwesiseko seseli-nge-seli.Ivumela uhlalutyo olunzulu kunye olupheleleyo lwenkqubo kwiiseli ezintsonkothileyo, apho ubukhulu becala inqanda ukufihla ukungafani kwazo ngokuthatha umndilili wazo zonke iiseli.

Nangona kunjalo, ezinye iiseli azifanelekanga ukuba zenziwe zibe yi-single-cell suspension, ngoko ke ezinye iindlela zokulungiselela iisampulu-i-nucleus extraction evela kwizicubu ziyafuneka, oko kukuthi, i-nucleus ikhutshwa ngokuthe ngqo kwiithishu okanye kwiseli kwaye ilungiswe ibe yi-single-nucleus suspension for single- ulandelelwano lweseli.

I-BMK inikezela nge-10 × i-Genomics yeChromium TM esekelwe kwinkonzo yokulandelelana kwe-RNA yeseli enye.Le nkonzo isetyenziswe ngokubanzi kwizifundo kwizifundo ezinxulumene nesifo, ezifana nokwahlukana kweeseli zomzimba, i-tumor heterogeneity, uphuhliso lwezicubu, njl.

I-Spatial transcriptome chip: 10× Genomics

Iqonga: I-Illumina NovaSeq Platform

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Umgaqo wobugcisa

Ukuhlukaniswa kwe-nuclei kufezekiswa yi-10 × i-Genomics Chromium TM, equkethe inkqubo ye-microfluidics ye-channel esibhozo kunye nokuwela kabini.Kule nkqubo, i-gel beads kunye ne-barcodes kunye ne-primer, i-enzymes kunye ne-nucleus enye ifakwe kwi-nanoliter-size drop drop oil, eyenza i-Gel Bead-in-Emulsion (GEM).Nje ukuba i-GEM yenziwe, i-cell lysis kunye nokukhululwa kweebhakhowudi zenziwa kwi-GEM nganye.I-mRNA ikhutshelwe umva kwiimolekyuli ze-cDNA ezineekhowudi ze-10× kunye ne-UMI, ezixhomekeke ngakumbi kulwakhiwo lwethala leencwadi olulandelelanayo.

Izicubu azifanelanga ukulungiswa kweseli enye

Iseli / Izicubu	Isizathu
Ithishu engumkhenkce engatshazi	Ayikwazi ukufumana imibutho emitsha okanye egcinwe ixesha elide
Iseli yezihlunu, iMegakaryocyte, Amafutha…	Idayamitha yeseli inkulu kakhulu ukuba ingene kwisixhobo
Isibindi...	I-ethe-ethe kakhulu ukuba yophuka, ayikwazi ukwahlula iiseli enye
Iseli yeNeuron, Ubuchopho...	Ubuthathaka ngakumbi, kulula ukucinezela, kuya kutshintsha iziphumo zokulandelelana
I-Pancreas, i-thyroid ...	Isityebi kwi-endogenous enzymes, echaphazela ukuveliswa kokumiswa kweseli enye

I-nucleus enye vs iseli enye

I-nucleus enye	Iseli enye
Unlimited cell diameter	Ubukhulu beseli: 10-40 μm
Izinto eziphathekayo zinokukhenkcezwa izicubu	Izinto eziphathekayo kufuneka zibe zizicubu ezitsha
Uxinzelelo oluphantsi lweeseli ezifriziwe	Unyango lwe-enzyme lunokubangela ukusabela koxinzelelo lweseli
Akukho ziseli ezibomvu zegazi kufuneka zisuswe	Iiseli ezibomvu zegazi kufuneka zisuswe
Inyukliya ivakalisa ulwazi lwebhayoloji	Iseli yonke ivakalisa ulwazi ngebhayoloji

IiNgcaciso zeNkonzo

Ithala leencwadi

Isicwangciso sokulandelelana

Umthamo weDatha

IiMfuno zeSampulu

Izicubu

I-10 × ilayibrari yeGenomics enye-nuclei

10x Genomics -Illumina PE150

100,000 efundwayo/iseli malunga.100-200 Gb

Inombolo yeSeli: >2×10⁵

Iseli conc.kwi-700-1,200 iseli / μL

≥ 200 mg

Ukufumana iinkcukacha ezithe vetshe malunga nesikhokelo sokulungiselela isampula kunye nokuhamba komsebenzi wenkonzo, nceda uzive ukhululekile ukuthetha nayeBMKGENE ingcaphephe