I-Genomics yokuthelekisa
Iingenelo zeNkonzo
●Ubungcali obuninzi kunye neerekhodi zokupapasha: ngeqoqo eliqokelelweyo, i-BMKGene igqibe iiprojekthi ze-genomics ezingaphezu kwama-90, kunye ne-collective impact factor efikelele kuma-900.
●Uhlalutyo olupheleleyo lwe-bioinformatics: iphakheji yohlalutyo iqulethe uhlalutyo olusibhozo oluqhelekileyo olufunekayo, olubonelela ngamanani ayilwe kakuhle alungele ukupapashwa kwaye luvumela ukutolikwa okulula kweziphumo
●Iqela le-bioinformatics elinobuchule obuphezulu kunye nomjikelo omfutshane wohlalutyo: ngamava amahle kuhlalutyo lwe-genomics oluthelekisayo, iqela le-BMKGene lihlangabezana neemfuno ezahlukeneyo zohlalutyo lomntu ngamnye ngexesha elifutshane.
●Inkxaso emva kokuthengisa:Ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.
Iinkcukacha zeNkonzo
| Ixesha eliqikelelweyo lokujika | Inani leentlobo zezilwanyana | Uhlalutyo |
| Iintsuku ezingama-30 zokusebenza | 6 - 12 | Ukuhlanganiswa kweentsapho ze-gene Ukwandiswa kunye nokuncitshiswa kosapho lwe-gene Ukwakhiwa kwemithi ye-phylogenetic Uqikelelo lwexesha lokwahlukana (Kufuneka ukulinganiswa kweefosili) Ixesha lokufaka i-LTR (Kwizityalo) Ukuphindaphinda kwe-genome epheleleyo (Kwizityalo) Uxinzelelo olukhethiweyo Uhlalutyo lwe-synteny |
Uhlalutyo lwe-Bioinformatics
● Usapho lwe-gene
● I-Phylogenetics
● Ixesha lokwahlukana
● Uxinzelelo olukhethiweyo
● Uhlalutyo lwe-synteny
Iimfuno zeSampuli kunye nokuHanjiswa
Iimfuno zeSampuli:
Izicubu okanye i-DNA yokulandelelanisa nokuhlanganisa i-genome
Kwizicwili
| Iintlobo zezilwanyana | Izicubu | Uphando | I-PacBio CCS |
| Isilwanyana | Izicubu ze-visceral | 0.5 ~ 1 g | ≥ 3.5 g |
| Izicubu zemisipha | |||
| ≥ 5.0 g | |||
| ≥ 5.0 mL | |||
| Igazi lezilwanyana ezincelisayo | |||
| ≥ 0.5 mL | |||
| Inyama yenkukhu/Igazi leentlanzi | |||
| Isityalo | Igqabi Elitsha | 1 ~ 2 g | ≥ 5.0 g |
| Ipetali/Isiqu | 1 ~ 2 g | ≥ 10.0 g | |
| Ingcambu/Imbewu | 1 ~ 2 g | ≥ 20.0 g | |
| Iiseli | Iseli ekhuliswe kakuhle | - | ≥ 1 x 108 |
Idatha
Iifayile zolandelelwano lwe-genome (.fasta) kunye neefayile ze-annotation (.gff3) zeentlobo ezinxulumeneyo ngokusondeleyo
Ukuhamba komsebenzi wenkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampulu
Ukwakhiwa kweThala leeNcwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zasemva kokuthengisa
*Iziphumo zedemo eziboniswe apha zonke zivela kwii-genomes ezipapashwe ngeBiomarker Technologies
1. Uqikelelo lwexesha lokufaka i-LTR: Lo mfanekiso ubonise usasazo olulodwa lwe-bimodal kumaxesha okufaka i-LTR-RTs kwi-Weining rye genome, xa kuthelekiswa nezinye iintlobo. Incopho yamva nje yabonakala malunga neminyaka eyi-0.5 yezigidi eyadlulayo.
ULi Guang nabanye,I-Nature Genetics, 2021
2. Uhlalutyo lwe-Phylogeny kunye nosapho lwe-gene kwi-chayote (Sechium edule): Ngokuhlalutya i-chayote kunye nezinye iintlobo ezili-13 ezinxulumene nayo kusapho lwe-gene, i-Chayote ifunyenwe inxulumene kakhulu ne-snake gourd (Trichosanthes anguina). I-Chayote ithathwe kwi-snake gourd malunga ne-27-45 Mya kunye ne-whole genome duplication (WGD) yabonwa kwi-chayote kwi-25±4 Mya, esisiganeko sesithathu se-WGD kwi-cucuibitaceae.
UFu A nabanye,Uphando lwezoLimo, 2021
3. Uhlalutyo lwe-synteny: Ezinye ii-genes ezinxulumene ne-phytohormones ekuphuhlisweni kweziqhamo zifunyenwe kwi-chayote, i-snake gourd kunye ne-squash. Ulwalamano phakathi kwe-chayote kunye ne-squash luphezulu kancinci kunolo luphakathi kwe-chayote kunye ne-snake gourd.
UFu A nabanye,Uphando lwezoLimo, 2021
4. Uhlalutyo losapho lwe-Gene: Ukutyetyiswa kwe-KEGG ekwandeni nasekuncipheni kosapho lwe-gene kwi-genomes ze-G.thurberi kunye ne-G.davidsonii kubonise ukuba i-steroid biosynthesis kunye ne-brassinosteroid biosynthesis ezinxulumene ne-genomes zandisiwe.
UYang Z nabanye.,Ibhayoloji ye-BMC, 2021
5. Uhlalutyo lokuphindaphinda i-genome epheleleyo: Uhlalutyo lokusasazwa kwe-4DTV kunye ne-Ks lubonise isiganeko sokuphindaphinda i-genome yonke. Iincopho ze-intraspecies zibonise iziganeko zokuphindaphinda. Iincopho ze-interspecies zibonise iziganeko ze-speciation. Uhlalutyo lubonise ukuba xa kuthelekiswa nezinye iintlobo ezintathu ezinxulumeneyo, i-O. europaea idlule kwi-gene replication enkulu kutshanje.
URao G nabanye.,Uphando lwezoLimo, 2021
Ityala le-BMK
Irozi engenamaqhuqhuva: ukuqonda kwe-genomic okunxulunyaniswa nokulungelelaniswa komswakama
Ipapashwe: Uphononongo lweSayensi yeSizwe, 2021
Icebo lokulandelelanisa:
'Basye'sI-Thornless' (R.I-Wichurainan) i-genome:
Malunga ne-93 X PacBio + malunga ne-90 X Nanopore + 267 X Illumina
Iziphumo eziphambili
1. I-genome ye-R.wichuraiana esemgangathweni ophezulu yakhiwe kusetyenziswa iindlela zokulandelelana ezifundwe ixesha elide, ezivelisa i-assembly ye-530.07 Mb (Ubungakanani be-genome obuqikelelweyo babumalunga ne-525.9 Mb nge-flow cytometry kunye ne-525.5 nge-genome survey； I-Heterozygosity yayimalunga ne-1.03%). Amanqaku aqikelelweyo e-BUSCO ayeyi-93.9%. Xa ethelekiswa ne-"Old blush" (haploOB), umgangatho kunye nokuphelela kwale genome kuqinisekiswe yi-base single-base accuracy kunye ne-LTR assembly index (LAI=20.03). I-genome ye-R.wichuraiana inee-genes ze-protein coding ezingama-32,674.
2. Uhlalutyo oludibeneyo lwe-Multi-omics, oluquka i-genomics ethelekisayo, i-transcriptomics, uhlalutyo lwe-QTL lwabemi be-genetic, lutyhile ulwahlulo olubalulekileyo phakathi kuka-R. wichuraiana kunye noRosa chinensis. Kwakhona, ukwahluka kokubonakaliswa kwe-genes ezinxulumeneyo kwi-QTL kusenokwenzeka ukuba kunxulunyaniswa ne-stem prickle patterning.
Uhlalutyo oluthelekisayo lwe-genomics phakathi kwe-Basye's Thornless kunye ne-Rosa chinensis kuquka uhlalutyo lwe-synteny, iqela losapho lwe-gene, uhlalutyo lokwandiswa kunye nokuqunjelwa, lutyhile inani elikhulu lotshintsho, olunxulumene neempawu ezibalulekileyo kwiirozi. Ukwanda okukhethekileyo kusapho lwe-gene lwe-NAC kunye ne-FAR1/FRS kusenokwenzeka ukuba kunxulunyaniswe nokuxhathisa amabala amnyama.
Uhlalutyo lothelekiso lwe-genomics phakathi kwe-BT kunye ne-haploOB genomes.
UZhong, M., et al. “I-Rose without prickle: ukuqonda nge-genomic okunxulumene nokulungiswa komswakama”Uphononongo lweSayensi yeSizwe, 2021;, nwab092.
