Ulandelelwano lwe-Amplicon (16S/18S/ITS)

Ukulandelelana kwe-Amplicon (16S/18S/ITS) kunye ne-Illumina yindlela yokuhlalutya ukwahluka kweentsholongwane ngokuchonga iiprofayili zeentsholongwane ngokweendlela zazo zokulandelelana uze uzame ukuqonda ubutyebi boluntu kunye nokwahluka ngaphakathi kwesampuli nganye naphakathi kweesampuli. Umbhobho we-BMKCloud Amplicon (NGS) uvumela uhlalutyo lwee-16S, 18S, ITS kunye neejini ezininzi ezisebenzayo. Iqala ngokunciphisa ukufunda, ukuhlanganiswa kokufunda okudibeneyo kunye novavanyo lomgangatho, kulandelwa kukuhlanganiswa kokufundwa okufanayo ukuvelisa iiYunithi zeTaxonomic zoMsebenzi (ii-OTU) ezisetyenziswa kumacandelo amathandathu ahlukeneyo ohlalutyo. Inkcazo yeTaxonomic inika ulwazi malunga nobuninzi kunye nokwakheka kwesampuli nganye, ngelixa uhlalutyo lwe-alpha kunye ne-beta lokwahluka lugxila kukwahluka kweentsholongwane ngaphakathi naphakathi kweesampuli, ngokwahlukeneyo. Uhlalutyo lokwahluka phakathi kwamaqela lufumana ii-OTU ezahlukayo kusetyenziswa uvavanyo lwe-parametric kunye nolwe-non-parametric, ngelixa uhlalutyo lokuhambelana lunxulumanisa olu mahluko kwizinto ezingqongileyo. Okokugqibela, ubuninzi beejini ezisebenzayo buqikelelwa ngokusekelwe kubuninzi beejini eziphawulayo, zibonelela ngengqiqo malunga nomsebenzi kunye ne-ecology kwisampuli nganye.