Uhlalutyo lwe-mRNA ye-Eukaryotic (Ukhetho olusekelwe kwisalathiso kunye nolwe-devo olutsha luyafumaneka)
Lo mbhobho usebenzisa idatha ye-NGS RNA-Seq njengengeniso kwaye uvelisa iziphumo ezivela kuhlalutyo oluninzi oluya ngasemva kuquka kodwa kungaphelelanga apho: ukulandelelanisa uvavanyo lomgangatho wedatha,de novoisichazi-magama sendawo yokubhala, uhlalutyo lokudibanisa izinto eziguquguqukayo, uhlalutyo lokubonakaliswa komahluko, isichazi-magama somsebenzi kunye nohlalutyo lokuphucula.
Uhlalutyo lwe-RNA olude olungasebenzisi ikhowudi
IiRNA ezinde ezingabhalisiyo iikhowudi (i-lncRNA) zii-transcripts ezingabhalisiyo iikhowudi ezinobude obungaphezulu kwe-200 nt kwaye zaziwa ngokudlala indima ekuhleleni nasekulawuleni i-chromatin. Iitekhnoloji zokulandelelanisa ezisebenzisa i-high-throughput kunye ne-bioinformatics zisinike amandla okuqonda ulandelelwano lwe-lncRNA kunye nolwazi lokubeka ukuze sichonge ii-lncRNA ezinemisebenzi ebalulekileyo yokulawula. Le pipeline inika uhlalutyo lwe-lncRNA ukongeza kuhlalutyo olukhankanyiweyo kwi-Eukaryotic mRNA Analysis pipeline.
Ulandelelwano lwe-amplicon ye-16S/18S/ITS
Umjelo wohlalutyo lokwahluka kweentsholongwane ze-Amplicon Sequencing waphuhliswa ngokusekelwe kwiminyaka yamava kuhlalutyo lweprojekthi yokwahluka kweentsholongwane. Lo mjelo uqulathe uhlalutyo olusisiseko oluqhelekileyo, olugubungela umxholo wohlalutyo oluphambili lophando lwangoku lweentsholongwane kunye nohlalutyo lomntu ngamnye. Ingxelo yohlalutyo ityebile kwaye ibanzi, kunye nokukhetha ukwenza uhlalutyo lomntu olwahlukeneyo. Ukongeza, iisampulu kunye namaqela anokutshintshwa ngephanyazo ukuze kwenziwe uhlengahlengiso olongezelelweyo kunye nolawulo.
Uhlalutyo lweShotgun Metagenomics
Umbhobho woHlalutyo lweMetagenomic weShotgun usebenzisa idatha ye-NGS evela kwizinto ezixutyiweyo ze-genomic ezithathwe kwiisampuli zokusingqongileyo. Uhlalutyo oluqukiweyo lubonelela ngolwazi oluneenkcukacha malunga nokwahluka kweentlobo kunye nobuninzi bazo, ulwakhiwo lwabemi, ubudlelwane be-phylogenetic, ii-genes ezisebenzayo, kunye nothungelwano lokunxibelelana nezinto zokusingqongileyo.
Uhlalutyo loHluko lwe-NGS-WGS
Uhlalutyo lwe-NGS-WGS Variant luluhlu oludibeneyo lokuchonga utshintsho olusebenzayo olwenza ulawulo lomgangatho wedatha, ulungelelwaniso lolandelelwano, uhlalutyo, kunye nohlalutyo lokuguqulwa kwezakhi zofuzo. Olu luhlu lulandela iindlela ezilungileyo ze-GATK zokufumanisa i-SNP kunye ne-InDel kwaye lusebenzisa iManta ekubizeni utshintsho oluqulunqwe ngendlela yesakhiwo.
Uphononongo lwe-Genome Wide Association (GWAS)
Umbhobho weGWAS luhlalutyo oluya ngasemva oluthatha iifayile zeVCF ezenziwe ngaphambili kunye nedatha yephenotype ehambelanayo kwiqela labantu njengengeniso. Isebenzisa iindlela ezithile zezibalo, iGWAS ijolise ekufumaneni umahluko we-nucleotide kwi-genome enxulumene nomahluko we-phenotypic. Idlala indima ebalulekileyo ekuphononongeni ii-genes ezisebenzayo ezinxulumene nezifo ezintsonkothileyo zabantu kunye neempawu ezintsonkothileyo kwizityalo nakwizilwanyana.
Uhlalutyo lokuHlulwa koBuninzi (i-BSA)
Uhlalutyo lwe-BSA lubandakanya ukuhlanganisa abantu abaneempawu ezingaqhelekanga ze-phenotypic kuluntu olwahlulahlulayo. Ngokuthelekisa indawo eyahlukileyo phakathi kweesampuli ezidityanisiweyo, le ndlela ifumanisa ngokukhawuleza iimpawu zemolekyuli ezinxulumene kakhulu ne-genes ekujoliswe kuzo. Isetyenziswa kakhulu kwimephu yemfuza yezityalo nezilwanyana, sisixhobo esibalulekileyo sokuzalanisa ngoncedo lwe-marker-assisted.
Uhlalutyo lwe-Evolutionary Genetics
Umsebenzi we-Evolutionary Genetics Analysis usebenzisa amava amaninzi e-BMKGENE kwiiprojekthi ze-genetic evolution kwaye uquka ukwakhiwa kwemithi ye-phylogenetic, uhlalutyo lwe-linkage disequilibrium, uvavanyo lokwahlukahlukana kwe-genetic, ukuchonga okukhethiweyo, uhlalutyo lwe-floorship, uhlalutyo lwe-principal component, kunye nokuchazwa kwesakhiwo sabemi.
