BMKCloud Ngena

Iimveliso

Ulandelelwano lweMetatranscriptome

Itekhnoloji yokulandelelanisa i-Illumina, inkonzo yolandelelwano ye-metaranscriptome ye-BMKGENE ityhila ukubonakaliswa kofuzo oluguquguqukayo lweentlobo ngeentlobo zeentsholongwane, ezithatha ii-eukaryotes ukuya kwiiprokaryotes kunye neentsholongwane, ngaphakathi kwendawo yendalo efana nomhlaba, amanzi, ulwandle, isitulo, kunye namathumbu. Inkonzo yethu ebanzi ixhobisa abaphandi ukuba baphengulule kwiiprofayile ezipheleleyo zegenesis yegenesis yasekuhlaleni. Ngaphandle kohlalutyo lwe-taxonomic, inkonzo yethu yolandelelwano ye-metatranscriptome iququzelela ukuphononongwa ekutyebiseni okusebenzayo, ikhanyisela iigenes ezichazwe ngokwahlukileyo kunye neendima zabo. Fumanisa ubutyebi bengqiqo yebhayoloji njengoko ujonga kumhlaba ontsonkothileyo wokuchazwa kofuzo, iyantlukwano yetaxonomic, kunye nokuguquguquka okusebenzayo ngaphakathi kwezi niches zahlukeneyo zokusingqongileyo.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iimpapasho ezikhoyo

Iimpawu zeNkonzo

● Ukuncipha kwe-rRNA elandelwa lulungiselelo lwethala leencwadi le-mRNA eliqondisayo.

● Ulandelelwano kwi-Illumina NovaSeq.

Iinkonzo eziluncedo

●Funda iiNguquko zoLuntu lweMicrobial:Oku kwenzeka kwinqanaba lokukhutshelwa kwaye kuphonononge imfuza enokubakho emitsha.

●Ukucacisa iNtsebenziswano yoLuntu lweMicrobial kunye noMmkeli okanye iNdawo.

●Uhlalutyo oluBanzi lwe-Bioinformatic: Oku kubonelela ngengqiqo malunga netaxonomic yoluntu kunye neengoma ezisebenzayo, kunye nohlalutyo oluhlukeneyo lwegama lofuzo.

●Inkcazo yemfuza eBanzi:Ukusetyenziswa kogcino-lwazi oluhlaziyiweyo lwemfuza yemfuza yolwazi lwenkcazelo yemfuza yoluntu lwemicrobial.

●Inkxaso yasemva kwentengiso:Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.

IiNgcaciso zeNkonzo

Iqonga lokulandelelana	Isicwangciso solandelelwano	Idatha iyacetyiswa	Ulawulo loMgangatho weDatha
Illumina NovaSeq	PE150	I-12Gb	Q30≥85%

IiMfuno zeSampulu

Ugxininiso (ng/µL)	Imali iyonke (µg)	Umthamo (µL)	OD260/280	OD260/230	I-RIN
≥50	≥1.0	≥20	1.8-2.0	1.0-2.5	≥6.5

Ukuhamba komsebenzi weNkonzo

Ukuhanjiswa kwesampuli

Ukwakhiwa kwethala leencwadi

Ulandelelwano

Uhlalutyo lwedatha

Iinkonzo emva kokuthengisa

Ngaphambili: De novo Fungal Genome Assembly

Okulandelayo: Prokaryotic RNA Ulandelelwano

Ibandakanya uhlalutyo olulandelayo:

● Ukulandelelanisa uLawulo loMgangatho weDatha

● INdibano yokuBhala

● I-Taxonomic Annotation kunye ne-Abundance

● Isihlomelo esiSebenzayo kunye noBuninzi

● Ubungakanani beNgcaciso kunye nokuHlalutya okwahlukileyo

Usasazo lwerhafu kwisampulu nganye:

Uhlalutyo lokwahluka kweBeta: UPGMA

Inkcazo esebenzayo - GO ngobuninzi

Ubuninzi betaxonomy eyahlukileyo - LEFSE

Phonononga inkqubela phambili eququzelelwa yi-BMKGene's meta transcriptomics yolandelelwano lweenkonzo ngengqokelela egciniweyo yopapasho.

Lu, Z. et al. (2023) 'Ukunyamezelwa kwe-asidi ye-lactate-usebenzisa iibhaktheriya zomyalelo I-Bacteroidales inegalelo ekuthinteleni i-ruminal acidosis kwiibhokhwe eziqhelaniswe nokutya okugxininise kakhulu',Ukutya kwezilwanyana, 14, iphepha 130-140. doi: 10.1016/J.ANINU.2023.05.006.

Ingoma, Z. et al. (2017) 'Ukutyhila i-microbiota engundoqo esebenzayo kwi-fermentation yesiko eliqinileyo nge-amplicon ephezulu kunye nokulandelelana kwe-metaranscriptomics',Imida kwiMicrobiology, 8(JUL). doi: 10.3389/FMICB.2017.01294/FULL.

Wang, W. et al. (2022) 'iNoveli yeMycoviruses eFunyaniswe kuPhando lweMetatranscriptomics yePhytopathogenic Alternaria Fungus',Iintsholongwane, 14(11), iphe. 2552. doi: 10.3390/V14112552/S1.

Wei, J. et al. (2022) 'Uhlalutyo lwe-metatranscriptome oluhambelanayo lubonisa ukuthotywa kwe-metabolites yesibini yesityalo ngooqongqothwane kunye ne-gut symbionts',Imolekyuli I-Ecology, 31(15), iphepha 3999-4016. doi: 10.1111/MEC.16557.