i-mRNA-seq (NGS) ene-genome ebhekisayo
I-RNA-seq sisixhobo esiqhelekileyo kuzo zonke iisayensi zobomi kunye nezityalo, sivala umsantsa phakathi kwee-genomes kunye neeproteomes. Amandla ayo akwiindlela zokufumana ii-transcripts ezintsha kunye nokulinganisa ukubonakaliswa kwazo kuvavanyo olunye. Isetyenziswa kakhulu kwizifundo zokuthelekisa ii-transcriptomic, ikhanyisa ii-genes ezinxulumene neempawu ezahlukeneyo okanye ii-phenotypes, njengokuthelekisa ii-mutants kwiintlobo zasendle okanye ukutyhila ukubonakaliswa kwe-gene phantsi kweemeko ezithile. I-BMKCloud mRNA(Reference) APP idibanisa ukulinganiswa kokubonakaliswa, uhlalutyo lokubonakaliswa kokwahluka (DEG), kunye nohlalutyo lwesakhiwo solandelelwano kwipayipi ye-mRNA-seq(NGS) bioinformatics kwaye idibanisa amandla esoftware efanayo, iqinisekisa ukuba kulula ukuyisebenzisa. Abasebenzisi banokulayisha idatha yabo ye-RNA-seq kwilifu, apho i-App inikezela ngesisombululo sohlalutyo lwe-bioinformatic esibanzi, esisisigxina. Ukongeza, ibeka phambili amava abathengi, inikezela ngemisebenzi eyenzelwe wena ngokweemfuno ezithile zabasebenzisi. Abasebenzisi banokuseta iiparameter kwaye bangenise umsebenzi wepayipi ngokwabo, bajonge ingxelo esebenzisana nabo, bajonge idatha/iidayagram kunye nokumbiwa kwedatha epheleleyo, okufana: ukukhetha i-gene ekujoliswe kuyo, ukuqokelela okusebenzayo, ukudweba iidayagram, njl.
Iqonga:I-Illumina, MGI
Icebo:I-RNA-Seq
Uyilo: Idatha ecocekileyo, ecocekileyo.
Uhlobo lweThala leeNcwadi:i-fr-unstranded, i-fr-firststrand okanye i-fr-secondstrand
Ubude bokufunda:150 bp
Uhlobo lwefayile:*.fastq, *.fq, *.fastq.gz okanye *.fq.gz. Inkqubo iyadibanisa ngokuzenzekelayo iifayile ze-.fastq ngokwegama leefayile zazo,umz. *_1.fastq idityaniswe ne *._2.fastq.
Inani leesampuli:Akukho mida kwinaniyesampuli, kodwa ixesha lohlalutyo liya kwanda njengoko inaniiisampulu ziyakhula.
Isixa seDatha esicetyiswayo:6G ngesampulu nganye
