Eukaryotic MRNA Sequening-ng
Mawonekedwe
● Kugwidwa kwa MRNA isanayambe kukonzekera laibulale
● Kukonzekera njira yoyendetsera Mrna la Mrna kuti muthandize kupeza deta yocheperako
● Kusanthula kwa Bioninformatic
Ubwino
●Waluso kwambiri: Gulu lathu lakonza zitsanzo zopitilira 600,000, kupondaponda mitundu yosiyanasiyana monga zikhalidwe zam'manja, zimakhala, komanso madzi amthupi. Timabweretsa chuma chambiri pantchito iliyonse ndipo tamaliza bwino ntchito zoposa 100,000 za A Seq mu kafukufuku wosiyanasiyana.
●Chiwongolero cholimba: Timakhazikitsa zowongolera zapamwamba pamagawo onse, kuchokera ku zitsanzo komanso kukonzekera laibulale kuti tisanthule ndi bioinformatictictic. Kuwunika kosangalatsa kumeneku kumeneku kumatsimikizira zotsatira za zotsatira zanthawi zonse, kukutsimikizirani kuti polojekiti yanu ili m'manja odalirika.
●Chimodzimodzi: Timagwiritsa ntchito madabwa angapo kuti mulengeze majini osiyanasiyana (degs) ndikuchita zowunikira zophatikizika. Njira zokwanirazi zimapereka chidziwitso mu ma cell am'manja ndi molecular poyankha, kuonetsetsa kuti mumadziwitsidwa pazotsatira zanu.
●Thandizo la Kugulitsa Pambuyo: Kudzipereka kwathu kumapitilira kumaliza ntchito polojekiti 3 - patatha miyezi itatu kapena nthawi yogulitsa. Panthawi imeneyi, timapereka thandizo lotsatira, kuvutitsa, ndi magawo kuti athetse mafunso okhudzana ndi zotsatira zake.
Zofunikira ndi Kutumiza
| Nkhokwe ya mabuku | Njira Yolowetsa | Zambiri Zotsimikiziridwa | Kuwongolera kwapadera |
| Mwamphamvu | Audina Pe150 DNbseq-T7 | 6 -10 GB | Q30≥05% |
Zofunikira:
Nucleotides:
| . (Zg / μl) | Kuchuluka (μg) | Kukhala Uliwala | Umunthu | |
| Laibulale ya muyezo | ≥ 10 | ≥ 0.2 | Ode260 / 280 = 1.7-2.5 Ode260 / 230 = 0,5-2.5 Ma protein kapena opanda mapuloteni kapena kuipitsa kwa DNA kuwonetsedwa pa gel. | Kwa zomera: Rin≥4.0; Za nyama: Rin≥4.5; 5.0 $28s / 18s≥1.0; Ochepa kapena okwera pa Baserine |
| Laibulale yowongolera | ≥ 10 | ≥ 0.2 | Ode260 / 280 = 1.7-2.5 Ode260 / 230 = 0,5-2.5 Ma protein kapena opanda mapuloteni kapena kuipitsa kwa DNA kuwonetsedwa pa gel. | Kwa zomera: Rin≥4.0; Za nyama: Rin≥4.5; 5.0 $28s / 18s≥1.0; Ochepa kapena okwera pa Baserine |
● Zomera:
Muzu, tsinde kapena petal: 450 mg
Tsamba kapena mbewu: 300 mg
Zipatso: 1.2 g
●Nyama:
Mtima kapena matumbo: 300 mg
Viscera kapena ubongo: 240 mg
Minofu: 450 mg
Mafupa, tsitsi kapena khungu: 1g
● Arthropods:
Tizilombo: 6g
Crustacea: 300 mg
● Magazi onse: 1 chubu
● Maselo: 106 malulowa
Kulimbikitsidwa Kwambiri
Chidebe: 2 ml centrifuge chubu (chinimba cha tin sichikulimbikitsidwa)
Kuyika zitsanzo: Gulu + litasintha mwachitsanzo A1, A2, A3; B1, B2, B3 ....
Kutumiza:
1. Ma ayezi oundana: zitsanzo zimayenera kunyamula m'matumba ndikuyikidwa m'manda oundana.
2. Machubu a RNAS: RNA Samati amawuma mu RNA kukhazikika chubu (mwachitsanzo RNASTRE®) ndikutumizidwa mu kutentha kwa firiji.
Ntchito Yogwira Ntchito
Mapangidwe a kuyesa
Kutumiza kwachitsanzo
Kutulutsa kwa RNA
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Bioinfoctic
Eukaryotic Mrna Sequening Perclow
Bioinfoctic
ØZolemba za data
ØKuyanjana kwa Gene
ØKusanthula kwa Shipcript
ØMawu owerengera
ØKusanthula kosiyanasiyana
ØKuthana ndi ntchito komanso zolemetsa
Kuyanjana kwa Gene
Kutalika kwa data
Kuphatikiza kwachitsanzo ndi kuwunika kwa zofananira kwachilengedwe
Makina omveka bwino (degs)
Kugwiritsa Ntchito Manja
Zopindulitsa
Funsani zomwe zikugwirizana ndi BMKGECE NGS NGS AMSNAQE.
Huang, L. et al. . 119736. Doi: 10016 / J.watres.2023.1197336.
Jia, LJ et al. . Doi: 10,1016 / J.choom.2023.02.002.
Jin, K. et al. (2022) 884443. Doi: 10.3389 / FPLS.2022.884443 / BIBULO.
Wen, x. et al. .
Zhang, Yujie et al. . 101798. Doi: 10016 / J.023.101798.
