Zogulitsa

Eukaryotic mRNA Sequencing-Illumina

Kutsatizana kwa mRNA kumathandizira kuti ma mRNA onse alembedwe kuchokera ku ma cell pamikhalidwe inayake.Ndi ukadaulo wamphamvu kwambiri wowulula mawonekedwe a jini, kapangidwe kake ndi mamolekyu azinthu zina zachilengedwe.Mpaka pano, kutsatizana kwa mRNA kwagwiritsidwa ntchito kwambiri pakufufuza kofunikira, zowunikira zamankhwala, chitukuko cha mankhwala, ndi zina zambiri.

Pulatifomu: Illumina NovaSeq Platform

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Ubwino wake

● Odziwa Kwambiri: Zitsanzo zoposa 200,000 zasinthidwa mu BMK zomwe zikuphatikizapo mitundu yosiyanasiyana ya zitsanzo, kuphatikizapo chikhalidwe cha maselo, minofu, madzi a m'thupi, ndi zina zotero.

● Dongosolo lokhazikika loyang'anira khalidwe labwino: Malo oyendetsera khalidwe lapakati pamasitepe onse kuphatikizapo kukonzekera zitsanzo, kukonzekera laibulale, kutsatizana ndi bioinformatics akuyang'aniridwa mosamala kuti apereke zotsatira zapamwamba.

● Malo angapo osungira omwe alipo kuti afotokoze ntchito ndi maphunziro owonjezera kuti akwaniritse zolinga zosiyanasiyana za kafukufuku.

● Ntchito zogulitsa pambuyo pa malonda: Ntchito zogulitsa pambuyo pake ndizovomerezeka kwa miyezi 3 polojekiti ikamalizidwa, kuphatikizapo kutsata mapulojekiti, kuthetsa mavuto, zotsatira za Q & A, ndi zina zotero.

Zitsanzo Zofunika ndi Kutumiza

Library	Njira yotsatirira	Deta yovomerezeka	Kuwongolera Kwabwino
Poly A yowonjezera	Chithunzi cha PE150	6gb pa	Q30≥85%

Zitsanzo Zofunika:

Nucleotides:

Conc.(ng/μl)

Mtengo (μg)

Chiyero

Umphumphu

≥ 20

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel.

Kwa zomera: RIN≥6.5;

Kwa nyama: RIN≥7.0;

5.0≥28S/18S≥1.0;

kukwera kochepa kapena kopanda koyambira

Thupi: Kulemera (kuuma):≥1 g
*Pa minofu yaing'ono kuposa 5 mg, timalimbikitsa kutumiza zitsanzo za minofu yowuma (mu madzi a nayitrogeni).

Kuyimitsidwa kwa cell:Chiwerengero cha ma cell = 3 × 10⁶-1 × 10⁷
*Tikupangira kutumiza ma cell a frozen lysate.Ngati selolo likhala laling'ono kuposa 5 × 105.

Zitsanzo za magazi:Kuchuluka ≥1 ml

Microorganism:Kulemera ≥ 1 g

Kutumiza Kwachitsanzo Kovomerezeka

Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)

Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3;B1, B2, B3...

Kutumiza:

Dry-ice: Zitsanzo ziyenera kupakidwa m'matumba ndikukwiriridwa mu ayezi wouma.
RNAstable machubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa mu firiji.