ØOdziwika Kwambiri: Zitsanzo zopitilira 200,000 zasinthidwa mu BMK zomwe zikukhudza mitundu yosiyanasiyana, kuphatikiza chikhalidwe cha maselo, minofu, madzi amthupi, ndi zina zambiri.
ØDongosolo lokhazikika loyang'anira khalidwe: Malo owongolera khalidwe lapakati pamasitepe onse kuphatikizapo kukonzekera zitsanzo, kukonzekera laibulale, kutsatizana ndi bioinformatics akuyang'aniridwa kuti apereke zotsatira zapamwamba kwambiri.
ØMa database angapo omwe amapezeka kuti afotokoze ntchito ndi maphunziro owonjezera kuti akwaniritse zolinga zosiyanasiyana za kafukufuku.
ØNtchito zogulitsa pambuyo pogulitsa: Ntchito zogulitsa pambuyo pake ndizovomerezeka kwa miyezi itatu polojekiti ikamalizidwa, kuphatikiza kutsata ma projekiti, kuwombera zovuta, zotsatira za Q&A, ndi zina.
Library | Njira yotsatirira | Deta yovomerezeka | Kuwongolera Kwabwino |
Poly A yowonjezera | Chithunzi cha PE150 | 6gb pa | Q30≥85% |
Nucleotides:
Chiyero | Umphumphu | Ndalama |
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel. | Zazomera: RIN≥6.5;Zanyama: RIN≥7;28S/18S≥1.0; kukwera kochepa kapena kulibe koyambira | Conc.≥30 ng/μl;Kuchuluka ≥ 10 μl;Zonse ≥ 1.5 μg |
Thupi: Kulemera (kuuma):≥1 g
*Pa minofu yaing'ono kuposa 5 mg, timalimbikitsa kutumiza zitsanzo za minofu yowuma (mu madzi a nayitrogeni).
Kuyimitsidwa kwa cell:Chiwerengero cha ma cell = 3 × 106-1 × 107
*Tikupangira kutumiza frozen cell lysate.Ngati cellyo ikhala yaying'ono kuposa 5 × 105, kung'anima kozizira mu nayitrogeni wamadzimadzi kumalimbikitsidwa, komwe kumakhala koyenera kutulutsa pang'ono.
Zitsanzo za magazi:Kuchuluka ≥1 ml
Microorganism:Kulemera ≥ 1 g
Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)
Zitsanzo zolembera: Gulu+ lobwereza mwachitsanzo A1, A2, A3;B1, B2, B3...
Kutumiza:
Bioinformatics
Eukaryotic mRNA sequencing kusanthula kayendedwe ka ntchito
Bioinformatics
ØKuwongolera khalidwe la data yaiwisi
ØReference genome alignment
ØKusanthula kapangidwe ka zilembo
ØKuchulukitsa kwa mawu
ØKusanthula kwamawu osiyanasiyana
ØTanthauzo la ntchito ndi kuwonjezera
1.mRNA Data Saturation curve
2.Differential expression analysis-Volcano plot
3.Ndemanga za KEGG pa DEGs
4.GO classification pa DEGs