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Genome-wide Association Analysis
Genome-wide association study (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imakhudzana ndi makhalidwe enaake (phenotype).Kafukufuku wa GWAS amafufuza zolembera zamtundu wamtundu wonse wa anthu ambiri ndikulosera mayanjano a genotype-phenotype posanthula ziwerengero pamlingo wa anthu.Zakhala zikugwiritsidwa ntchito kwambiri pofufuza za matenda a anthu ndi migodi yogwira ntchito ya majini pa makhalidwe ovuta a nyama kapena zomera.
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Single-nucleus RNA Sequencing
Kutsogola kwa kujambula kwa selo limodzi ndi njira yopangira laibulale yamunthu payekha kuphatikiza ndi kutsata kopitilira muyeso kumathandizira maphunziro amtundu wa gene pa cell-by-cell.Imathandizira kusanthula kozama komanso kokwanira pama cell ovuta, momwe imapewa kubisala kusiyanasiyana kwawo potenga pafupifupi ma cell onse.
Komabe, ma cell ena sali oyenera kupangidwa kuti akhale kuyimitsidwa kwa selo limodzi, kotero njira zina zokonzekera zitsanzo - kuchotsa nyukiliya kuchokera ku minofu kumafunika, ndiko kuti, nyukiliya imachotsedwa mwachindunji ku minofu kapena selo ndikukonzedwa kuti ikhale imodzi-nucleus kuyimitsidwa kwa single- kutsatizana kwa ma cell.
BMK imapereka 10 × Genomics Chromium TM yochokera ku cell single RNA sequencing service.Utumikiwu wagwiritsidwa ntchito kwambiri pamaphunziro okhudzana ndi matenda, monga kusiyanitsa kwa maselo a chitetezo chamthupi, chotupa chosiyana, kukula kwa minofu, ndi zina zambiri.
Spatial transcriptome chip: 10 × Genomics
Platform: Illumina NovaSeq 6000
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Kutsata kwamtundu wa Zomera/Zinyama Zonse
Kusinthasintha kwamtundu wonse, komwe kumadziwikanso kuti WGS, kumathandizira kuwulula masinthidwe wamba komanso osowa pamtundu wonsewo kuphatikiza Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), ndi Copy Number Variation (CNV). ).Ma SVs amapanga gawo lalikulu la kusiyana kosiyana kusiyana ndi SNPs ndipo zimakhudza kwambiri majeremusi, zomwe zimakhudza kwambiri zamoyo.Kuwerengeranso kwanthawi yayitali kumathandizira kuzindikirika bwino kwa zidutswa zazikulu komanso kusiyanasiyana kovutirapo chifukwa kuwerenga kwautali kumapangitsa kuti zikhale zosavuta kuwoloka madera ovuta monga kubwereza tandem, madera olemera a GC/AT, ndi zigawo zosinthika kwambiri.
Platform: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Kulinganiza kwa malo kwa maselo kumagwira ntchito yofunika kwambiri pazochitika zosiyanasiyana zamoyo, monga kulowetsedwa kwa chitetezo cha mthupi, kukula kwa mwana wosabadwayo, ndi zina zotero. Kutsatizana kwa malo, komwe kumasonyeza mbiri ya jini ndikusunga zambiri za malo, kwapereka chidziwitso chambiri mu kamangidwe ka minofu ya transcriptome.Ndi chitukuko chaukadaulo, mawonekedwe owoneka bwino a minofu ndi kusiyana kwenikweni kwamawonekedwe amtundu wa ma cell amayenera kuphunziridwa bwino kwambiri.BMKGENE imapereka ntchito yokwanira, yoyimitsa malo amodzi kuchokera ku zitsanzo kupita ku chidziwitso chachilengedwe.
Matekinoloje a Spatial transcriptomics adapatsa mphamvu malingaliro atsopano m'mabwalo osiyanasiyana ofufuza pothetsa mbiri ya jini yokhala ndi zopezeka m'magawo osiyanasiyana.
Spatial transcriptome chip: BMKMANU S1000
Platform: Illumina NovaSeq 6000
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10x Genomics Visium Spatial Transcriptome
Visium Spatial Gene Expression ndi ukadaulo wapadziko lonse wotsatizana wa transcriptome pakuyika minofu kutengera mRNA yonse.Lembani zolemba zonse zokhala ndi mawonekedwe a morphological kuti mupeze zidziwitso zatsopano zakukula kwabwinobwino, matenda a matenda, ndi kafukufuku womasulira wamatenda.BMKGENE imapereka ntchito yokwanira, yoyimitsa malo amodzi kuchokera ku zitsanzo kupita ku chidziwitso chachilengedwe.
Matekinoloje a Spatial transcriptomics adapatsa mphamvu malingaliro atsopano m'mabwalo osiyanasiyana ofufuza pothetsa mbiri ya jini yokhala ndi zopezeka m'magawo osiyanasiyana..
Spatial transcriptome chip: 10x Genomics Visium
nsanja:Illumina NovaSeq 6000
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Kutalika Kwathunthu kwa mRNA Sequencing-Nanopore
Kutsatizana kwa RNA kwakhala chida chamtengo wapatali chowunikira mwatsatanetsatane zolembalemba.Mosakayikira, kutsatizana kwachikale kwa kuwerenga mwachidule kunapindula zambiri zofunika pano.Komabe, nthawi zambiri imakumana ndi zoletsa pakuzindikiritsa kwautali wa isoform, kuchuluka, kukondera kwa PCR.
Nanopore sequencing imadzisiyanitsa ndi nsanja zina zotsatizana, chifukwa ma nucleotides amawerengedwa mwachindunji popanda kaphatikizidwe ka DNA ndipo amapanga kuwerengera nthawi yayitali pa makumi a kilobases.Izi zimapereka mphamvu zowerengera molunjika ndikuwoloka zolembedwa zazitali zonse ndikuthana ndi zovuta mumaphunziro amlingo wa isoform.
nsanja:Nanopore PromethION
Laibulale:cDNA-PCR
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Kutsata kutalika kwa mRNA -PacBio
Pa novokutsatizana kwa ma transcriptome kutalika, komwe kumadziwikanso kutiPa novoIso-Seq imatenga ubwino wa PacBio sequencer mu kutalika kwa kuwerenga, zomwe zimathandiza kutsatizana kwa ma molekyulu a cDNA aatali popanda kusweka.Izi zimapewa kwathunthu zolakwika zilizonse zomwe zimapangidwa pamasitepe amisonkhano yolembera ndikupanga ma seti a unigene okhala ndi isoform-level resolution.Ma seti a unigenewa amapereka chidziwitso champhamvu cha majini monga "reference genome" pa transcriptome-level.Kuphatikiza apo, kuphatikiza ndi deta yotsatizana ya m'badwo wotsatira, ntchitoyi imapatsa mphamvu kuwerengera kolondola kwa mawu amtundu wa isoform.
Pulatifomu: PacBio Sequel IILibrary: SMRT belu laibulale -
Eukaryotic mRNA Sequencing-Illumina
Kutsatizana kwa mRNA kumathandizira kuti mbiri ya mRNA yonse yolembedwa kuchokera ku cell ikhale yodziwika.Ndi ukadaulo wamphamvu kwambiri wowulula mawonekedwe a jini, kapangidwe kake ndi mamolekyu azinthu zina zachilengedwe.Mpaka pano, kutsatizana kwa mRNA kwagwiritsidwa ntchito kwambiri pakufufuza kofunikira, zowunikira zamankhwala, chitukuko cha mankhwala, ndi zina zambiri.
Platform: Illumina NovaSeq 6000
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Non-Reference based mRNA Sequencing-Illumina
Kutsatizana kwa mRNA kumatengera njira yotsatirira ya m'badwo wotsatira (NGS) kuti ijambule messenger RNA(mRNA) mawonekedwe a Eukaryote panthawi inayake yomwe ntchito zina zapadera zikuyatsidwa.Zolemba zazitali kwambiri zomwe zidapangidwa zimatchedwa 'Unigene' ndipo zimagwiritsidwa ntchito ngati njira yotsatirira pakuwunikiridwa kotsatira, yomwe ndi njira yabwino yophunzirira momwe mamolekyu amayendera komanso maulamuliro amtundu wamtunduwu popanda kutchulapo.
Pambuyo pa kusonkhanitsa deta ya transcriptome ndi kumasulira kwa unigene
(1) Kusanthula kwa SSR, kuneneratu kwa CDS ndi kapangidwe ka jini zidzakonzedweratu.
(2) Kuchulukitsa kwa mawu a unigene mu chitsanzo chilichonse kudzachitidwa.
(3) Ma unigene omwe amawonetsedwa mosiyanasiyana pakati pa zitsanzo (kapena magulu) adzapezeka potengera mawu osagwirizana
(4) Kuphatikizika, kutanthauzira kogwira ntchito ndi kusanthula kopindulitsa kwa ma unigene ofotokozedwa mosiyanasiyana kudzachitika
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Kutsata kwanthawi yayitali kosalemba-Illumina
Ma RNA autali osalemba zilembo (lncRNAs) ndi mtundu wa mamolekyu a RNA okhala ndi kutalika kopitilira 200 nt, omwe amadziwika ndi kuthekera kotsika kwambiri.LncRNA, monga membala wofunikira mu ma RNA osalemba ma code, amapezeka makamaka mu nucleus ndi plasma.Kukula kwa ukadaulo wotsatizana ndi bioinformatics kumathandizira kuzindikira ma LncRNA angapo ndikuphatikiza omwe ali ndi ntchito zachilengedwe.Umboni wochuluka umasonyeza kuti lncRNA imakhudzidwa kwambiri ndi epigenetic regulation, transcript regulation ndi post-transcription regulation.
