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Single-nucleus RNA Sequencing
Kutsogola kwa kujambula kwa selo limodzi ndi njira yopangira laibulale yamunthu payekha kuphatikiza ndi kutsata kopitilira muyeso kumathandizira maphunziro amtundu wa gene pa cell-by-cell.Imathandizira kusanthula kozama komanso kokwanira pama cell ovuta, momwe imapewa kubisala kusiyanasiyana kwawo potenga pafupifupi ma cell onse.
Komabe, ma cell ena sali oyenera kupangidwa kukhala kuyimitsidwa kwa selo limodzi, kotero njira zina zokonzekera zitsanzo - kuchotsa ma nucleus kuchokera ku minofu kumafunika, ndiko kuti, nyukiliya imachotsedwa mwachindunji ku minofu kapena selo ndikukonzedwa kuti ikhale imodzi-nucleus kuyimitsidwa kwa single- kutsatizana kwa ma cell.
BMK imapereka 10 × Genomics Chromium TM yochokera ku cell single RNA sequencing service.Utumikiwu wagwiritsidwa ntchito kwambiri pamaphunziro okhudzana ndi matenda, monga kusiyanitsa kwa maselo a chitetezo chamthupi, chotupa chosiyana, kukula kwa minofu, ndi zina zambiri.
Spatial transcriptome chip: 10 × Genomics
Pulatifomu: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Spatial transcriptomics imatsogola pazatsopano zasayansi, kupatsa mphamvu ofufuza kuti afufuze movutikira momwe ma jini amafotokozera mkati mwa minofu ndikusunga malo awo.Pakati pa nsanja zosiyanasiyana, BMKGene yapanga BMKManu S1000 Spatial Transcriptome Chip, modzitamandira.chiganizo chowonjezerekaya 5µM, kufika pamtundu wa subcellular, ndikuthandiziramakonda amitundu yambiri.Chip cha S1000, chokhala ndi mawanga pafupifupi 2 miliyoni, chimagwiritsa ntchito tinthu tating'onoting'ono tomwe timakhala ndi mikanda yodzaza ndi zojambulidwa za barcode.Laibulale ya cDNA, yopangidwa ndi ma barcode a malo, imakonzedwa kuchokera ku chipangizo cha S1000 ndikutsatiridwa papulatifomu ya Illumina NovaSeq.Kuphatikiza kwa zitsanzo zokhala ndi barcode ndi UMIs zimatsimikizira kulondola ndi kutsimikizika kwa deta yopangidwa.Khalidwe lapadera la BMKManu S1000 chip lili mu kusinthasintha kwake, lopereka zosintha zamitundu ingapo zomwe zimatha kusinthidwa bwino kumitundu yosiyanasiyana komanso mwatsatanetsatane.Kusinthasintha kumeneku kumayika chip ngati chisankho chabwino kwambiri pamaphunziro osiyanasiyana amtundu wapamalo, kuwonetsetsa kusanja kolondola kwa malo popanda phokoso lochepa.
Pogwiritsa ntchito chipangizo cha BMKManu S1000 ndi matekinoloje ena a spatial transcriptomics, ofufuza atha kumvetsetsa bwino momwe ma cell amagwirira ntchito komanso kuyanjana kwa ma cell komwe kumachitika mkati mwa minyewa, ndikupereka chidziwitso chamtengo wapatali pamakina omwe amachokera kuzinthu zachilengedwe m'magawo osiyanasiyana, kuphatikiza. oncology, neuroscience, chitukuko cha biology, immunology ndi maphunziro a botanical.
Platform: BMKManu S1000 chip ndi Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics ndiukadaulo wotsogola womwe umalola ofufuza kuti afufuze momwe ma jini amatchulidwira mkati mwa minofu ndikusunga malo awo.Pulatifomu imodzi yamphamvu mderali ndi 10x Genomics Visium yophatikizidwa ndi kutsatizana kwa Illumina.Mfundo ya 10X Visium yagona pa chip chapadera chokhala ndi malo ojambulidwa omwe zigawo za minofu zimayikidwa.Malo ogwidwawa ali ndi mabala a barcode, aliwonse ofanana ndi malo apadera mkati mwa minofu.Mamolekyu a RNA omwe adagwidwa kuchokera m'minyewayo amalembedwa ndi zozindikiritsa mamolekyulu (UMIs) panthawi yolemba mobwereza.Malo okhala ndi barcode awa ndi ma UMI amathandizira kupanga mapu olondola a malo komanso kuchuluka kwa mafotokozedwe a jini pakusintha kwa selo limodzi.Kuphatikiza kwa zitsanzo zokhala ndi barcode ndi UMIs zimatsimikizira kulondola ndi kutsimikizika kwa deta yopangidwa.Pogwiritsa ntchito ukadaulo uwu wa Spatial Transcriptomics, ofufuza amatha kumvetsetsa mozama momwe ma cell amakhalira komanso momwe ma cell amagwirira ntchito mkati mwa minyewa, ndikupereka chidziwitso chamtengo wapatali pamakina omwe amachokera kuzinthu zachilengedwe m'magawo angapo, kuphatikiza oncology, neuroscience, biology yachitukuko, chitetezo chamthupi. , ndi maphunziro a botanical.
Platform: 10X Genomics Visium ndi Illumina NovaSeq
