Metagenomic Sequencing -NGS
Metagenome ndi mndandanda wazinthu zonse zopezeka m'magulu osiyanasiyana a zamoyo, monga chilengedwe ndi anthu. Lili ndi ma genomes omwe amalimidwa komanso osalimidwa. Shotgun metagenomic sequencing ndi NGS imathandizira kusanthula kwamitundu yodabwitsayi yophatikizidwa mu zitsanzo zachilengedwe popereka zambiri kuposa mbiri ya taxonomic, kuperekanso chidziwitso chaching'ono cha mitundu yosiyanasiyana ya zamoyo, kuchuluka kwa mphamvu, ndi kuchuluka kwa kuchuluka kwa anthu. Kupitilira pa maphunziro a taxonomic, shotgun metagenomics imaperekanso mawonekedwe owoneka bwino a genomics, omwe amathandizira kuwunika kwa majini osungidwa ndi maudindo awo okhazikika pazachilengedwe. Pomaliza, kukhazikitsidwa kwa maukonde olumikizana pakati pa ma genetic ndi zinthu zachilengedwe kumathandizira kumvetsetsa kwathunthu kuyanjana kwapakati pamagulu ang'onoang'ono ndi chilengedwe chawo. Pomaliza, kutsatizana kwa metagenomic kumakhala ngati chida chofunikira kwambiri powunikira zovuta zamitundu yosiyanasiyana yamagulu ang'onoang'ono amitundu yosiyanasiyana, ndikuwunikira ubale wosiyanasiyana pakati pa majini ndi chilengedwe mkati mwazachilengedwe zovutazi.
Mapulatifomu: Illumina NovaSeq ndi DNBSEQ-T7
Ubwino wa Utumiki
●Njira Yodzipatula ndi Kulima Yopanda Mbiri Yambiri yamagulu a Microbial: Kuthandizira kutsatizana kwa ma genetic kuchokera ku zamoyo zosalimidwa.
●Kukhazikika Kwambiri: Dziwani mitundu yocheperako mu zitsanzo zachilengedwe.
●Comprehensive Bioinformatics Analysis:Sanangoyang'ana pa kusiyana kwa taxonomic komanso kusiyana kwa magwiridwe antchito ammudzi.
●Zochitika Zazambiri:Pokhala ndi mbiri yakutseka bwino mapulojekiti angapo a metagenomics m'magawo osiyanasiyana ofufuza ndikukonza zitsanzo zopitilira 200,000, gulu lathu limabweretsa zokumana nazo zambiri pantchito iliyonse.
Mafotokozedwe a Utumiki
| Sequencing nsanja | Njira Yotsatirira | Deta yovomerezeka | Kuwongolera khalidwe |
| Illumina NovaSeq kapena DNBSEQ-T7 | Mtengo wa PE150 | 6-20Gb | Q30≥85% |
Zofunikira pa Utumiki
| Kukhazikika (ng/µL) | Ndalama zonse (ng) | Voliyumu (µL) |
| ≥1 | ≥30 | ≥20 |
● Dothi / matope: 2-3g
● Zinyama za m'mimba: 0.5-2g
● Zomwe zili m'matumbo-tizilombo: 0.1-0.25g
● Pamwamba pa zomera (matope olemera): 0.5-1g
● Msuzi wowotchera wowonjezera matope): 0.2-0.5g
● Nyasi (zinyama zazikulu): 0,5-2g
● Nyasi (mbewa): 3-5mbewu
● Pulmonary alveolar lavage fluid: pepala losefera
● Kupukuta maliseche: 5-6 swabs
● Khungu/kumaliseche/kumaliseche/malovu/mnofu wofewa m'kamwa/pharyngeal swab/nsomba: 2-3 swabs
● Tizilombo tating'onoting'ono: 5-6 swabs
● Waterbody/air/biofilm: pepala losefera
● Endophytes: 2-3g
● Mpanda Wamano: 0.5-1g
Kuyenda kwa Ntchito Yantchito
Kupereka zitsanzo
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Mulinso zowunikira izi:
● Kutsata kuwongolera khalidwe la data
● Kukonzekera kwa Metagenome ndi kuneneratu za majini
● Mawu a jini
● Kusanthula kwamitundu yosiyanasiyana ya alpha
● Kusanthula kogwira ntchito kwa anthu ammudzi: biological function, metabolic, antibiotic resistance
● Kuwunika kwa mitundu yogwira ntchito komanso yataxonomic:
Kusanthula kwamitundu yosiyanasiyana ya Beta
Kusanthula kwamagulu
Kusanthula kwa mgwirizano: pakati pa zinthu zachilengedwe ndi kapangidwe ka OUT ndi kusiyanasiyana
Kusanthula ntchito: CARD antibiotic resistance
Kusanthula kosiyana kwa KEGG metabolic pathways: heatmap of key pathways
Kusiyanasiyana kwa Alpha kwa Kugawa kwa taxonomic: ACE index
Mitundu ya Beta yogawa taxonomic: PCoA
Onani kupita patsogolo kotsogozedwa ndi BMKGene's metagenome sequencing services ndi Illumina kudzera mgulu lazofalitsa.
Hai, Q. et al. (2023) 'Kusanthula kwa metagenomic ndi metabolomic kwa kusintha kwa matumbo a utawaleza (Oncorhynchus mykiss) omwe ali ndi kachilombo koyambitsa matenda a hematopoietic necrosis pamitundu yosiyanasiyana yamadzi kutentha',Frontiers mu Microbiology, 14, p. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Madera ang'onoang'ono, ma jini otsutsa, ndi zoopsa zotsutsana ndi nyanja zam'tawuni zamitundu yosiyanasiyana ya trophic: Maulalo amkati ndi zikoka zakunja', (2023)Journal of Hazardous Materials Advans,9, p. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Metagenomic Analysis Yavumbulutsa Kusiyana Kwa Mapangidwe ndi Ntchito Pakati pa Tizilombo Zogwirizana ndi Liquid ndi Zolimba za Nkhosa Rumen', (2022)Frontiers mu Microbiology, 13, p. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Obese Ningxiang pig-derived microbiota rewires carnitine metabolism kulimbikitsa minofu yamafuta acid kukhala mu nkhumba zowonda za DLY',The Innovation, 4(5), p. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Zidziwitso za Metagenomic pazangozi zomwe zingakhalepo za pulasitiki woyimira bio/osawonongeka ndi zinyalala zosakhala pulasitiki kumtunda ndi kumunsi kwa Haihe Estuary, China',Sayansi ya The Total Environment,887 p. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
