mRNA-seq (NGS) yokhala ndi genome yofotokozera
RNA-seq ndi chida chodziwika bwino pa sayansi ya zamoyo ndi mbewu, chomwe chimatseka kusiyana pakati pa majini ndi ma proteome. Mphamvu yake ili pakupeza zolemba zatsopano ndikuyesa momwe zimaonekera muyeso umodzi. Imagwiritsidwa ntchito kwambiri pamaphunziro oyerekeza a transcriptomic, kuwunikira majini okhudzana ndi makhalidwe osiyanasiyana kapena ma phenotypes, monga kufananiza zosinthika ndi mitundu yachilengedwe kapena kuwulula mawonekedwe a majini pansi pa mikhalidwe inayake. BMKCloud mRNA(Reference) APP imaphatikiza kuchuluka kwa mafotokozedwe, kusanthula kwa mafotokozedwe osiyana (DEG), ndi kusanthula kwa kapangidwe ka sequence mu pipeline ya mRNA-seq(NGS) bioinformatics ndikuphatikiza mphamvu za mapulogalamu ofanana, kuonetsetsa kuti ogwiritsa ntchito ndi osavuta kugwiritsa ntchito. Ogwiritsa ntchito amatha kukweza deta yawo ya RNA-seq ku mtambo, komwe Pulogalamuyi imapereka yankho lathunthu, lokhazikika la bioinformatic analysis. Kuphatikiza apo, imayika patsogolo zomwe makasitomala amakumana nazo, kupereka ntchito zomwe zimakonzedwa kuti zigwirizane ndi zosowa za ogwiritsa ntchito. Ogwiritsa ntchito amatha kukhazikitsa magawo ndikupereka ntchito ya pipeline okha, kuyang'ana lipoti lolumikizana, kuwona deta/ma diagram ndikupeza deta yonse, monga: kusankha majini ofunikira, kusonkhanitsa ntchito, kujambula zithunzi, ndi zina zotero.
Nsanja:Illumina, MGI
Njira:RNA-Seq
Kapangidwe: Deta yoyera, yosalala.
Mtundu wa laibulale:fr-unstranded, fr-firststrand kapena fr-secondstrand
Utali wowerenga:150 bp
Mtundu wa fayilo:*.fastq, *.fq, *.fastq.gz kapena *.fq.gz. Dongosololi lidzateroSakanizani mafayilo a .fastq okha malinga ndi mayina a mafayilo awo,mwachitsanzo *_1.fastq yolumikizidwa ndi *._2.fastq.
Chiwerengero cha zitsanzo:Palibe zoletsa pa chiwerengerochozitsanzo, koma nthawi yosanthula idzawonjezeka pamene chiwerengero chazitsanzo zimakula.
Kuchuluka kwa Deta Koyenera:6G pa chitsanzo chilichonse
