Kutsata kwa Amplicon (16S/18S/ITS)

Kusanthula kwa Amplicon (16S/18S/ITS) ndi Illumina ndi njira yowunikira kusiyanasiyana kwa tizilombo toyambitsa matenda mwa kuzindikira ma profiles a tizilombo toyambitsa matenda motsatira ndondomeko zawo kenako kuyesa kuzindikira kulemera kwa anthu ammudzi ndi kusiyanasiyana mkati mwa chitsanzo chilichonse komanso pakati pa zitsanzo. Njira ya BMKCloud Amplicon (NGS) imalola kusanthula majini a 16S, 18S, ITS ndi majini ambiri ogwira ntchito. Imayamba ndi kudula kuwerenga, kusonkhana kwa kuwerenga kogwirizana ndi kuwunika kwa khalidwe, kutsatiridwa ndi kusonkhanitsa ma read ofanana kuti apange Ma Units Ogwira Ntchito (OTUs) omwe amagwiritsidwa ntchito m'magawo asanu ndi limodzi osiyanasiyana osanthula. Kufotokozera kwa Taxonomic kumapereka chidziwitso pa kuchuluka ndi kapangidwe ka chitsanzo chilichonse, pomwe kusanthula kwa alpha ndi beta kumayang'ana kwambiri kusiyanasiyana kwa tizilombo mkati ndi pakati pa zitsanzo, motsatana. Kusanthula kosiyana pakati pa magulu kumapeza ma OTU omwe amasiyana pogwiritsa ntchito mayeso a parametric ndi non-parametric, pomwe kusanthula kwa correlation kumagwirizanitsa kusiyana kumeneku ndi zinthu zachilengedwe. Pomaliza, kuchuluka kwa majini ogwira ntchito kumanenedweratu kutengera kuchuluka kwa majini odziwika, kupereka chidziwitso cha ntchito ndi chilengedwe mu chitsanzo chilichonse.