Amplicon Sequencing (16S/18S/ITS)

Amplicon (16S/18S/ITS) yotsatizana ndi Illumina ndi njira yowunikira kusiyanasiyana kwa tizilombo tating'onoting'ono pozindikira machulukidwe ang'onoang'ono molingana ndi kutsatizana kwawo ndikuyesa kuzindikira kuchuluka kwa anthu ammudzi ndi kusiyanasiyana mkati mwachitsanzo chilichonse komanso pakati pa zitsanzo. Mapaipi a BMKCloud Amplicon (NGS) amalola kusanthula kwa 16S, 18S, ITS ndi majini angapo ogwira ntchito. Zimayamba ndikuchepetsa kuwerenga, kuwerengera-kumaliza kuwerengera komanso kuwunika kwamtundu, kutsatiridwa ndi kuphatikiza zowerengera zofanana kuti apange Operational Taxonomic Units (OTUs) yomwe imagwiritsidwa ntchito m'magawo asanu ndi limodzi owunikira. Ndemanga za taxonomic zimapereka chidziwitso pakuchulukira kwachibale ndi kapangidwe kachitsanzo chilichonse, pomwe kusanthula kwa alpha ndi beta kumawunikira kusiyanasiyana kwa ma microbial mkati ndi pakati pa zitsanzo, motsatana. Kusanthula kosiyana pakati pamagulu kumapeza ma OTU omwe amasiyana pogwiritsa ntchito mayeso onse a parametric ndi osakhala a parametric, pomwe kusanthula kwamalumikizidwe kumakhudzana ndi kusiyana kumeneku kuzinthu zachilengedwe. Pomaliza, kuchuluka kwa majini ogwira ntchito kumanenedweratu kutengera kuchuluka kwa ma jini, zomwe zimapereka chidziwitso pakugwira ntchito ndi chilengedwe pachitsanzo chilichonse.