BMKCloud Lowani

Zogulitsa

Kutsata kwa Metatranscriptome

Tekinoloje yotsatizana ya Illumina, BMKGENE's metaranscriptome sequencing service ikuwulula mawonekedwe amtundu wamitundu yosiyanasiyana ya tizilombo tating'onoting'ono tosiyanasiyana, totengera ma eukaryotes mpaka ma prokaryotes ndi ma virus, m'malo achilengedwe monga dothi, madzi, nyanja, chimbudzi, ndi matumbo. Ntchito yathu yokwanira imapatsa mphamvu ofufuza kuti afufuze mbiri yamitundu yonse yamagulu ang'onoang'ono. Kupitilira kusanthula kwa taxonomic, ntchito yathu yotsatizana ya metatranscriptome imathandizira kufufuza pakulemeretsa kwa magwiridwe antchito, kuwunikira ma jini owonetsedwa mosiyanasiyana ndi maudindo awo. Dziwani zambiri zazachilengedwe mukamayang'ana mawonekedwe ovuta a ma gene, mitundu yosiyanasiyana ya taxonomic, komanso magwiridwe antchito mkati mwamitundu yosiyanasiyana yazachilengedwe.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Zofalitsa zotsatiridwa

Mawonekedwe a Utumiki

● Kuwonongeka kwa rRNA kutsatiridwa ndi kukonzekera laibulale ya mRNA yotsogolera.

● Kutsata pa Illumina NovaSeq.

Ubwino wa Utumiki

●Phunzirani Zosintha za Madera Ovuta a Microbial:Izi zimachitika pamlingo wolembera ndikuwunika majini atsopano omwe angakhalepo.

●Kufotokozera Microbial Community Interactions ndi Host kapena Environment.

●Comprehensive Bioinformatic Analysis: Izi zimapereka chidziwitso pamagulu amtundu wa taxonomic ndi magwiridwe antchito, komanso kusanthula kwamitundu yosiyanasiyana.

●Kufotokozera kwa Gene:Kugwiritsa ntchito nkhokwe zaposachedwa za jini kuti mudziwe zambiri zamafotokozedwe amtundu wamagulu ang'onoang'ono.

●Thandizo Pambuyo Pakugulitsa:Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.

Mafotokozedwe a Utumiki

Sequencing nsanja	Njira Yotsatirira	Deta yovomerezeka	Data Quality Control
Illumina NovaSeq	Mtengo wa PE150	12 Gb	Q30≥85%

Zitsanzo Zofunika

Kukhazikika (ng/µL)	Ndalama zonse (µg)	Voliyumu (µL)	OD260/280	OD260/230	RIN
≥50	≥1.0	≥20	1.8-2.0	1.0-2.5	≥6.5

Kuyenda kwa Ntchito Yantchito

Kupereka zitsanzo

Kumanga laibulale

Kutsata

Kusanthula deta

Pambuyo pogulitsa ntchito

Zam'mbuyo: Ndi Novo Fungal Genome Assembly

Ena: Kutsata kwa Prokaryotic RNA

Mulinso zowunikira izi:

● Kutsata Kuwongolera kwa Ubwino wa Data

● Transcript Assembly

● Taxonomic Annotation and Abundance

● Mawu Ogwira Ntchito ndi Kuchuluka

● Kutanthauzira Kwamawu ndi Kusanthula Kwamasiyana

Kugawa kwa taxonomic kwa chitsanzo chilichonse:

Kusanthula kwamitundu yosiyanasiyana ya Beta: UPGMA

Kafotokozedwe kantchito - GO kuchuluka

Kuchuluka kwamisonkho - LEFSE

Onani kupita patsogolo koyendetsedwa ndi BMKGene's meta transcriptomics sequencing services kudzera m'gulu lazofalitsa.

Lu, Z. et al. (2023) 'Kulekerera kwa asidi kwa mabakiteriya ogwiritsira ntchito lactate a dongosolo la Bacteroidales kumathandizira kupewa ruminal acidosis mu mbuzi zomwe zimasinthidwa kukhala chakudya chokhazikika',Chakudya Chanyama, 14, masamba 130-140. doi: 10.1016/J.ANINU.2023.05.006.

Nyimbo, Z. et al. (2017) 'Kuvumbulutsa core functional microbiota mu chikhalidwe cholimba-state fermentation ndi high-throughput amplicons ndi metatranscripttomics sequencing',Frontiers mu Microbiology, 8 (JUL). doi: 10.3389/FMICB.2017.01294/FULL.

Wang, W. et al. (2022) 'Novel Mycoviruses Anapezeka mu Survey ya Metatranscriptomics ya Phytopathogenic Alternaria Fungus',Ma virus, 14(11), p. 2552. doi: 10.3390/V14112552/S1.

Wei, J. et al. (2022) 'Kusanthula kofanana kwa metatranscriptome kumawonetsa kuwonongeka kwa ma metabolites achiwiri a chomera ndi kafadala ndi ma symbionts awo m'matumbo',Molecular Ecology, 31(15), masamba 3999-4016. doi: 10.1111/MEC.16557.