Zogulitsa

Genome-wide Association Analysis

Genome-wide association study (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imakhudzana ndi makhalidwe enaake (phenotype).Kafukufuku wa GWAS amafufuza zolembera zamtundu wamtundu wonse wa anthu ambiri ndikulosera mayanjano a genotype-phenotype posanthula ziwerengero pamlingo wa anthu.Zakhala zikugwiritsidwa ntchito kwambiri pofufuza za matenda a anthu ndi migodi yogwira ntchito ya majini pa makhalidwe ovuta a nyama kapena zomera.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Nkhani Yofalitsidwa

1.Ubwino wa Utumiki

● Milandu Yambiri ya Ntchito: Chiyambireni kukhazikitsidwa kwake mu 2009, BMKGENE yamaliza ntchito zambiri zamtundu wamtundu wafukufuku wa GWAS, idathandizira ochita kafukufuku kufalitsa nkhani zopitilira 100, ndipo kuchuluka kwamphamvu kwafika pa 500.
● Akatswiri ofufuza.
● Kusanthula kwakanthawi kochepa.
● Kusunga deta molondola.

2. Mafotokozedwe a Utumiki

Mtundu	Chiwerengero cha Anthu	Kutsata ndondomeko ndi kuya
SLAF-GWAS	Nambala yachitsanzo ≥200	Kukula kwa genome <400M, yokhala ndi ref-genome, WGS ndiyofunikira
		Kukula kwa genome ≤ 1G, 100K Tags ndi 10X
		1G ≤ Kukula kwa genome ≤ 2G, 200K Tags ndi 10X
		Kukula kwa genome> 2G, 300K Tags ndi 10X
WGS-GWAS	Nambala yachitsanzo ≥200	10X pachitsanzo chilichonse

3. Kusankha Zinthu

Mitundu yosiyanasiyana, mitundu yaying'ono, ma landraces / mabanki / mabanja osakanikirana / zinthu zakutchire

Mitundu yosiyanasiyana, subspecies, landraces

Half-sib family/full-sib family/zachilengedwe zakuthengo

4. Bioinformation Analysis

● Kusanthula mgwirizano wa genome-wide
● Kusanthula ndi kuwunika kwa SNP yofunikira
● Kafotokozedwe kake ka jini ya ofuna kusankha

5. Kuyenda kwa Ntchito ya Utumiki

Kuyesera kupanga

Kupereka zitsanzo

Kusintha kwa RNA

Kumanga laibulale

Kutsata

Kusanthula deta

Pambuyo pogulitsa ntchito

Zam'mbuyo: Single-nucleus RNA Sequencing

Ena:

a.Phenotype QC

Histogram yogawa pafupipafupi

Ziwerengero za Phenotype

b.Kusanthula kwamagulu (Model: GEMMA, FaST-LMM, EMMAX)

Chithunzi cha QQ

Manhattan Plot

Chaka	Journal	IF	Mutu
2022	NC	17.69	Genomic maziko a giga-chromosomes ndi giga-genome ya mtengo peony Paeonia ostii
2015	NP	7.43	Zolemba zapakhomo zimatsimikizira zigawo za genomic zofunika kwambiri mu soya
2018	MP	9.32	Kuphatikizika kwa ma genome amtundu wapadziko lonse lapansi wazinthu zogwiriridwa kumawulula maziko a chibadwa cha kusiyana kwawo kwa ecotype.
2022	HR	7.29	Kusanthula kwa genome-wide association kumapereka chidziwitso cha mamolekyu pakusintha kwachilengedwe kwa kukula kwa mbewu ya chivwende