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Gawani Chromatin-In Chromatin yopezeka ndi zigawenga zazitali (ATAC-seq)

Gawani Chromatin-Inlomatin yopezeka ndi zigawenga zaposanati (aTac-seq)

ATAC-Seq ndi njira yofananira yomwe imagwiritsidwa ntchito popenda kokwanira kwa chromatin. Imagwiritsa ntchito zimathandizira kumvetsetsa kwakukulu kwa njira zovuta za Egaine Unitric pa gene. Njira imagwiritsa ntchito tractive TNN Kukweza kwa PCR Kukwezedwa kumabweretsa zotsatira za laibulale yolowetsa, yomwe imalola kuzindikiritsa kwathunthu kwa madera otseguka a chromatin pansi pa malo apadera. ATac-Seq imaperekanso zochipangira malo opezeka malo abwino opezeka bwino, mosiyana ndi njira zomwe zimangoyang'ana pamasamba omasulira kapena zolemba zapamwamba. Potengera zigawo zotseguka izi zotseguka za chromatin, Atoc-seq ikuwonetsa madera omwe angagwiritse ntchito njira zomwe zimagwiritsidwa ntchito komanso zomwe zimayambitsa kusintha kwamasamba, kupereka socking yofunika kusinthasintha kwa majini a gene.

Lumikizanani nafe

Zambiri

Bioinfoctic

Zotsatira za Demo

Zolemba zolembedwa

Mawonekedwe antchito

● Kuchita kumafuna zitsanzo za minofu, m'malo mochotsa ma acid a ma acid, kuti asunge mgwirizano wa DNA-protein.

● AAC Njira imaphatikizapo kusungunuka kwa minofu, kutsatiridwa ndi nucleiction Eoiction, Chithandizo cha TN5 ndi kutsukidwa, Kusankhidwa kwa PCR, kukula.

● Sequenina mu Audina Novaseq

Ubwino Wantchito

●Chidwi chachikulu:Kukula kotsika kwa cell ndikokwanira pakukonzekera laibulale ndi kusungunuka.

●Njira Yothandiza Kwambiri: Nthawi yomweyo imafotokoza malo amitundu amitundu ya malo otseguka a chromatin ndi zinthu zogwira ntchito monga masamba ogwiritsira ntchito.

●Kupanga Kwabwino: Kuthamangitsa kwaukadaulo kumawonetsa ulemu wabwino kwambiri.

●Waluso kwambiri: Ndili ndi mazana a ntchito zotsekemera za ATAN adamaliza bwino, BMKGene amabweretsa zaka zambiri, gulu laukadaulo waluso, lofananira, komanso chithandizo chabwino kwambiri.

●Kuthekera kuti mulumikizane ndi kusanthula kwa ma transcriptomics: Kulola kusanthula kophatikizidwa kwa aTQ ndi data ina ya Amics monga RNA-seq.

● Kusanthula bwino kwa bioinoin: Kulepheretsa chizindikiritso cha zigawo zotseguka za chromatin ndi magwiridwe awo ogwirizana (oyang'anira, uts, exons) komanso kusanthula kwa kusiyana pakati pa zitsanzo.

Zolemba

Nkhokwe ya mabuku

Njira Yolowetsa

Zochita zotsimikizika deta

Kuwongolera kwapadera

Asac-seq

Audina Pe150

> 20m amawerenga

Kutengera kukula kwa genome (munthu: 50 m amawerenga)

Q30≥05%

Zofunikira Zitsanzo

Mtundu wa zitsanzo: minofu, maselo amoyo kapena oundana, magazi

● Nambala ya foni: ≥ 10⁶malulowa

● Kulemera kwa mitsempha: ≥ 200mg yatsopano

● Magazi: ≥ 4 ml

Ntchito Yogwira Ntchito

Mapangidwe a kuyesa

Kutumiza kwachitsanzo

DNA Kuchotsa

Ntchito Yomanga Laibulale

Kusazidza

Kusanthula kwa data

Ntchito Zogulitsa Pambuyo

M'mbuyomu: 16s / 18s / ampricon sequening-pacbio

Ena: Kusintha Kwambiri kwa Bsuvel Fisch (WGB)

Mulinso kuwunika:

● Kuwongolera kwa data.

● Peam kuyimbira potengera mapu a renome;

● NESI YOPHUNZITSIRA PAMODZI PAMODZI;

● Kusanthula kwa Pakeffe: Kuzindikiritsa masamba omasulira (TFB);

● Kusanthula kosiyanitsa ndi chizolowezi.

1.heap pa atoc imawerengera magawo a TSS ndi oyandikana nawo (± 3 KB)

2.Kodina kwa Chromatin Chromatin Regions mosiyanasiyana

1.Kuchentchentchess Peak kuyimbira pakati pamagulu

Onani ntchito yofufuza yomwe ili ndi ntchito za BMKGEN.

Fu, A.et al..Kafukufuku wa Hurtecil, 10 (1). Doi: 10,1093 / hr / uhac228.

Gong, B.et al.(2023) 'Epigenetiction ndi zolemba za Chinsinsi za Kinase Galt20C monga oncogene mu ggioma',NKHANI YA NJIRA NDI NYO, 50 (6), mas. 422-433. Doi: 10,1016 / J.jg.2023.01.008.

Iye, y.et al..Ziphuphu za Redox, 65, p. 102822. Doi: 10016 / J.reerdox.2023.102822.