Lowani mu BMKCloud

Zogulitsa

Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Kutsatana Kwambiri kwa Kudutsa (ATAC-seq)

ATAC-seq ndi njira yotsatirira kwambiri yomwe imagwiritsidwa ntchito pofufuza kupezeka kwa chromatin m'majini onse. Imagwiritsa ntchito kumvetsetsa kwakuya kwa njira zovuta zowongolera epigenetic padziko lonse lapansi pa kufotokozera majini. Njirayi imagwiritsa ntchito transposase ya hyperactive Tn5 kuti idule ndikutsegula madera a chromatin nthawi imodzi poika ma adapter a sequencing. Kukulitsa kwa PCR pambuyo pake kumapangitsa kuti pakhale laibulale ya sequencing, yomwe imalola kuzindikira kwathunthu madera otseguka a chromatin pansi pa mikhalidwe inayake ya space-time. ATAC-seq imapereka mawonekedwe onse a malo opezeka a chromatin, mosiyana ndi njira zomwe zimangoyang'ana malo omangirira zinthu zolembera kapena madera enaake osinthidwa ndi histone. Mwa kutsatirira madera otseguka a chromatin awa, ATAC-seq imavumbula madera omwe ali ndi mwayi wochita zinthu zolamulira komanso malo omwe angathe kumangirira zinthu zolembera, zomwe zimapereka chidziwitso chofunikira pakusintha kwamphamvu kwa kufotokozera majini kudutsa genome.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Chiwonetsero

Mabuku odziwika bwino

Zinthu Zofunika pa Utumiki

● Ntchito imafuna zitsanzo za minofu, m'malo mwa ma nucleic acid otulutsidwa, kuti isunge mgwirizano wa DNA ndi mapuloteni.

● Njira ya ATAC imaphatikizapo kugawikana kwa minofu, kutsatiridwa ndi kugawidwa kwa nuclei, chithandizo ndi kuyeretsa kwa Tn5, kukulitsa kwa PCR, kusankha kukula ndi kutsatana.

● Kutsatana kwa Illumina NovaSeq

Ubwino wa Utumiki

●Kuzindikira Kwambiri:Kuchuluka kochepa kwa maselo oyambira ndikokwanira kukonzekera ndi kutsata laibulale.

●Njira Yophunzitsa Kwambiri: nthawi yomweyo imavumbulutsa malo a genomic a chromatin yotseguka ndi zinthu zowongolera zogwira ntchito monga malo omangira zinthu zolembera.

●Kubwerezabwereza Kwabwino Koyesera: zobwerezabwereza zaukadaulo zikuwonetsa kubwerezabwereza kwabwino kwambiri.

●Ukatswiri Wochuluka: Ndi mapulojekiti mazana ambiri otsatizana a ATAC omwe atsirizidwa bwino, BMKGENE imabweretsa chidziwitso cha zaka zoposa khumi, gulu lofufuza laluso kwambiri, zomwe zili mkati, komanso chithandizo chabwino kwambiri pambuyo pogulitsa.

●Kuthekera Kogwirizana ndi Kusanthula kwa Transcriptomics: kulola kusanthula kophatikizana kwa ATAC seq ndi deta ina ya omics monga RNA-seq.

● Kusanthula Kwathunthu kwa Bioinformatics: zimathandiza osati kungozindikira madera otseguka a chromatin ndi magwiridwe antchito awo ofanana (othandizira, ma UT, ma exons, ma intron) komanso kusanthula kusiyana pakati pa madera otseguka a chromatin pakati pa zitsanzo.

Mafotokozedwe a Utumiki

Laibulale

Njira Yotsatizana

Zotsatira zodziwika bwino za deta

Kuwongolera khalidwe

ATAC-seq

Illumina PE150

> 20M kuwerenga

Kutengera kukula kwa majini (Munthu: 50 M amawerenga)

Q30≥85%

Zofunikira pa Zitsanzo

Mtundu wa zitsanzo: Minofu, maselo amoyo kapena oundana, magazi

● Nambala ya foni: ≥ 10⁶maselo

● Kulemera kwa minofu: ≥ 200mg minofu yatsopano

● Magazi: ≥ 4 mL

Kayendedwe ka Ntchito Yotumikira

Kapangidwe ka kuyesera

Kutumiza zitsanzo

Kuchotsa DNA

Kumanga laibulale

Kutsatana

Kusanthula deta

Ntchito zogulitsa pambuyo pogulitsa

Yapitayi: 16S/18S/ITS Amplicon Sequencing-PacBio

Ena: Kusanthula kwa bisulfite yonse ya genome (WGBS)

Kusanthula Kotsatiraku Kukuphatikizapo:

● Kuwongolera khalidwe la deta yosaphika;

● Kuyimba kwapamwamba kwambiri kutengera kulinganiza kwa majini ofunikira;

● Kufotokozera za jini pamalo okwera kwambiri;

● Kusanthula Motif: kuzindikira malo olumikizira zinthu zolembera (TFBS);

● Kusanthula ndi kufotokoza za kusiyana kwa ma peak.

1. Mapu a kutentha pa ATAC amawerenga kufalikira kwa TSS ndi madera oyandikana nawo (±3 kb)

2. Kufalikira kwa dera lotseguka la chromatin m'zigawo zosiyanasiyana za majini

3. Kusiyanasiyana kwa kuyitana kwapamwamba pakati pa magulu

Fufuzani kupita patsogolo kwa kafukufuku komwe kwathandizidwa ndi ntchito za BMKGene zotsatizana za ATAC kudzera mu zofalitsa zosiyanasiyana.

Fu, A.ndi ena.(2023) 'Kusonkhana kwa genome ya Telomere-to-telomere ya bitter melon (Momordica charantia L. var. abbreviata Ser.) kukuwonetsa kukula kwa zipatso, kapangidwe kake ndi mawonekedwe a majini okhwima',Kafukufuku wa Ulimi, 10(1). doi: 10.1093/HR/UHAC228.

Gong, B.ndi ena.(2023) 'Kuyambitsa kwa epigenetic ndi transcriptional kwa secretory kinase FAM20C monga oncogene mu glioma',Magazini ya Genetics ndi Genomics, 50 (6), tsamba 422-433. doi: 10.1016/J.JGG.2023.01.008.

Iye, Y.ndi ena.(2023) 'Butyrate imabwezeretsa kukana kwa ferroptosis mu khansa ya m'matumbo poyambitsa kuponderezedwa kwa c-Fos-dependent xCT',Redox Biology, 65, tsamba 102822. doi: 10.1016/J.REDOX.2023.102822.