Kutsata Kwathunthu kwa Transcriptome - Illumina
Mawonekedwe
● M'badwo wa laibulale wapawiri kuti utsatire zolemba zonse: laibulale yowonongeka ya rRNA ndi kukonzekera laibulale yaing'ono ya RNA.
● Kusanthula kwathunthu kwa bioinformamatic ya mRNA, lncRNA, circRNA ndi miRNA m'malipoti olekanitsidwa a bioinformamatic, kuphatikiza kusanthula kophatikizana ndi mawu onse a RNA ophatikizidwa, kuphatikiza kusanthula kwamanetiweki a ceRNA.
Ubwino wa Utumiki
●Katswiri Wazambiri: Takonza ma projekiti opitilira 2100 a transcriptome, okhala ndi mitundu yosiyanasiyana. Timabweretsa luso laukadaulo pantchito iliyonse.
●Ulamuliro Wabwino Kwambiri: Timagwiritsa ntchito mfundo zazikuluzikulu pamagawo onse, kuyambira kukonzekera zitsanzo mpaka kukonzekera laibulale, kutsatizana ndi bioinformatics. Kuwunika kwathu mosamalitsa kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.
●Mawu Omveka: Timagwiritsa ntchito nkhokwe zingapo kuti tifotokoze bwino za Mitundu Yowoneka Mosiyana (DEGs) ndikupanga kusanthula kofananirako. Njira yonseyi imakupatsirani zidziwitso zama cell ndi ma cell omwe amathandizira kuyankha kwa transcriptome, kuwonetsetsa kuti mumapeza zonse zomwe zingatheke pazoyeserera zanu.
●Kusanthula Mwakuya kwa Regulatory Networks: Kusanthula kwa netiweki ya ceRNA kumathandizidwa ndi kutsatana kophatikizana kwa mRNA, lncRNA, circRNA, ndi miRNA komanso ndi mayendedwe athunthu a bioinformamatic.
●Thandizo la Post-Sales: Timamvetsetsa kufunikira kopezeka, ndichifukwa chake kudzipereka kwathu kumapitilira kutha kwa ntchito ndi miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
Zitsanzo Zofunika ndi Kutumiza
| Library | Njira yotsatirira | Deta yovomerezeka | Kuwongolera Kwabwino |
| rRNA yatha | Chithunzi cha PE150 | 16 GB | Q30≥85% |
| Kukula kwasankhidwa | Illumina SE50 | 10-20M amawerengedwa |
Zitsanzo Zofunika:
Nucleotides:
| Conc.(ng/μl) | Mtengo (μg) | Chiyero | Umphumphu |
| ≥80 | ≥ 1.6 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel. | RIN≥6.0 5.0≥28S/18S≥1.0; kukwera kochepa kapena kopanda koyambira |
Kutumiza Kwachitsanzo Kovomerezeka
Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)
Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3; B1, B2, B3.
Kutumiza:
1. Dry-ice: Zitsanzo ziyenera kupakidwa m'matumba ndikukwiriridwa mu ayezi wouma.
2. RNAstable machubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa firiji.
Kuyenda kwa Ntchito Yantchito
Kuyesera kupanga
Kupereka zitsanzo
Kusintha kwa RNA
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Bioinformatics
Pamwamba pa kusanthula konse pamitundu ina iliyonse, mitundu inayi ya RNA imaphatikizidwa kusanthula kwathu kophatikizana:
- Kusanthula kwamaganizidwe pakati pa mitundu yonse yosiyanasiyana ya data ya RNA
- RNA chiwonetsero chazithunzi
- Chiwonetsero chamitundu yosiyanasiyana
- Malingaliro a kampani CeRNA Network
- Majini ofunikira ophatikizidwa munjira
- Kusanthula kasungidwe
- Mgwirizano wolunjika
RNA chiwonetsero chazithunzi
Majini Owonetsedwa Mosiyana
Kusanthula kwa ceRNA
Ma miRNA owonetsedwa mosiyanasiyana ndi ma RNA ogwirizana
Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene' yonse yotsatirira ma transcriptome kudzera pagulu lazofalitsa.
Dai, Y. et al. (2022) 'Mafotokozedwe omveka bwino a mRNAs, lncRNAs ndi miRNAs mu matenda a Kashin-Beck odziwika ndi RNA-sequencing',Molecular Omics, 18(2), tsamba 154-166. doi: 10.1039/D1MO00370D.
Liu, N. nan et al. (2022) 'Kusanthula kwanthawi zonse kwanthawi yayitali kwa Apis cerana kumapiri a Changbai panthawi yachisanu.',Gene, 830, masamba 146503-146503. doi: 10.1016/J.GENE.2022.146503.
Wang, XJ ndi al. (2022) 'Multi-Omics Integration-Based Preoritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Makhalidwe a Molecular and Drug Candidates',Malire mu Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al. (2022) 'Kusanthula kophatikizana kwa mbiri ya lncRNA/circRNA-miRNA-mRNA kumawulula zidziwitso zatsopano za njira zomwe zingatheke poyankha ma nematode a mizu mu peanut',BMC Genomics, 23(1), tsamba 1-12. doi: 10.1186/S12864-022-08470-3/ZINTHU/7.
Yan, Z. et al. (2022) 'Whole-transcriptome RNA sequencing ikuwonetsa njira zamamolekyu zomwe zimagwirizanitsidwa ndi kusungirako zokolola zaposachedwa mu broccoli ndi kuwala kofiira kwa LED',Biology ndi Technology ya Postharvest, 188, p. 111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
