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Kutsata Kwathunthu kwa Transcriptome - Illumina

Kutsata Kwanthawi Zonse - Chithunzi Chowonetsedwa ndi Illumina

Kutsatizana kwa zolemba zonse kumapereka njira yokwanira yolembera mamolekyu osiyanasiyana a RNA, kuphatikiza ma coding (mRNA) ndi ma RNA osalemba (lncRNA, circRNA, ndi miRNA). Njirayi imagwira zolemba zonse zama cell enieni panthawi inayake, ndikupangitsa kuti timvetsetse bwino njira zama cell. Zomwe zimatchedwanso "total RNA sequencing," cholinga chake ndi kuwulula maukonde owongolera pamlingo wa transcriptome, kuthandizira kusanthula mozama monga kupikisana kwa RNA (ceRNA) ndi kusanthula kwa RNA. Izi ndizomwe zimayambira poyambira kuzindikiritsa magwiridwe antchito, makamaka pakuwulula maukonde owongolera omwe akuphatikizana ndi circRNA-miRNA-mRNA-based ceRNA.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Zofalitsa Zowonetsedwa

Mawonekedwe

● Laibulale yapawiri kuti itsatire zolemba zonse: rRNA kutha motsatiridwa ndi kukonzekera laibulale ya PE150 ndi kusankha kukula kotsatiridwa ndi kukonzekera laibulale ya SE50

● Malipoti athunthu a bioinformatics a mRNA, lncRNA, circRNA, ndi miRNA m'malipoti osiyana a bioinformatics.

● Kusanthula kophatikizana kwa mawu onse a RNA mu lipoti lophatikizidwa, kuphatikiza kusanthula kwamanetiweki a ceRNA.

Ubwino wa Utumiki

●Kusanthula Mwakuya kwa Regulatory Networks: Kusanthula kwa netiweki ya ceRNA kumathandizidwa ndi kutsatana kophatikizana kwa mRNA, lncRNA, circRNA, ndi miRNA komanso ndi mayendedwe athunthu a bioinformamatic.

●Mawu Omveka: Timagwiritsa ntchito nkhokwe zingapo kuti tifotokozere za Differentially Expressed Genes (DEGs) ndikupanga kusanthula kofananirako, kupereka zidziwitso zama cell ndi mamolekyulu omwe amathandizira kuyankha kwa transcriptome.

●Katswiri Wazambiri: Pokhala ndi mbiri yakutseka bwino ma projekiti 2100 olembedwa m'magawo osiyanasiyana ofufuza, gulu lathu limabweretsa zokumana nazo zambiri pantchito iliyonse.

●Kuwongolera Kwabwino Kwambiri: Timagwiritsa ntchito mfundo zazikuluzikulu pamagawo onse, kuyambira pakukonza zachitsanzo ndi laibulale mpaka kutsatizana ndi bioinformatics. Kuyang'anitsitsa mosamalitsa kumeneku kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.

●Thandizo la Post-Sales: Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.

Zitsanzo Zofunika ndi Kutumiza

Library	Njira yotsatirira	Deta yovomerezeka	Kuwongolera Kwabwino
rRNA yatha	Chithunzi cha PE150	16 GB	Q30≥85%
Kukula kwasankhidwa	Illumina SE50	10-20M amawerengedwa	Q30≥85%

Zitsanzo Zofunika:

Nucleotides:

Conc.(ng/μl)

Mtengo (μg)

Chiyero

Umphumphu

≥80

≥ 1.6

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel.

RIN≥6.0

5.0≥28S/18S≥1.0;

kukwera kochepa kapena kopanda koyambira

Kutumiza Kwachitsanzo Kovomerezeka

Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)

Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3; B1, B2, B3.

Kutumiza:

1. Madzi owuma: Zitsanzo ziyenera kupakidwa m'matumba ndikukwiriridwa mu ayezi wouma.

2. RNAstable machubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa firiji.

Kuyenda kwa Ntchito Yantchito

Kuyesera kupanga

Kupereka zitsanzo

Kusintha kwa RNA

Kumanga laibulale

Kutsata

Kusanthula deta

Pambuyo pogulitsa ntchito

Zam'mbuyo: Chromatin Immunoprecipitation Sequencing (ChIP-seq)

Ena: CircRNA Sequencing-Illumina

Bioinformatics

RNA chiwonetsero chazithunzi

Majini Owonetsedwa Mosiyana

Kusanthula kwa ceRNA

Ma miRNA owonetsedwa mosiyanasiyana ndi ma RNA ogwirizana

Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene' yonse yotsatirira ma transcriptome kudzera pagulu lazofalitsa.

Dai, Y. et al. (2022) 'Mafotokozedwe omveka bwino a mRNAs, lncRNAs ndi miRNAs mu matenda a Kashin-Beck omwe amadziwika ndi RNA-sequencing', Molecular Omics, 18 (2), pp. 154-166. doi: 10.1039/D1MO00370D.

Liu, N. nan et al. (2022) 'Full length transcriptomes analysis of cold-resistance of Apis cerana in Changbai Mountain during overwintering period.', Gene, 830, pp. 146503–146503. doi: 10.1016/J.GENE.2022.146503.

Wang, XJ ndi al. (2022) 'Multi-Omics Integration-Based Preoritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Makhalidwe a Molecular and Drug Candidates', Frontiers in Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.

Xu, P. et al. (2022) 'Kusanthula kophatikizana kwa mbiri ya lncRNA/circRNA-miRNA-mRNA kumawulula zidziwitso zatsopano za njira zomwe zingatheke poyankha ma nematode a mizu mu peanut', BMC Genomics, 23 (1), pp. 1-12. doi: 10.1186/S12864-022-08470-3/ZINTHU/7.

Yan, Z. et al. (2022) 'Whole-transcriptome RNA sequencing ikuwonetsa njira zamamolekyu zomwe zimagwirizanitsidwa ndi kusungitsa khalidwe la pambuyo pokolola mu broccoli ndi kuwala kofiira kwa LED', Postharvest Biology and Technology, 188, p. 111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.