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Lintho tsa Proteomics
Proteomics e kenyelletsa ts'ebeliso ea mahlale a ho lekanya liprotheine tse teng ka har'a sele, lisele kapa ntho e phelang.Theknoloji e thehiloeng ho proteomics e sebelisoa ka mekhoa e fapaneng bakeng sa litlhophiso tse fapaneng tsa lipatlisiso tse kang ho lemoha matšoao a fapaneng a tlhahlobo, batho ba batlang tlhahiso ea ente, ho utloisisa mekhoa ea pathogenicity, ho fetola mekhoa ea ho bua ka lebaka la matšoao a fapaneng le tlhaloso ea litsela tsa protheine tse sebetsang mafung a fapaneng.Hajoale, litheknoloji tsa palo ea proteomics li arotsoe haholo ka maano a TMT, Label Free le DIA.
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Metabolomics
Metabolome ke sehlahisoa se ka tlase ho noka sa genome mme se na le tlatsetso e felletseng ea limolek'hule tsa boima bo tlase ba limolek'hule (metabolites) ka seleng, liseleng kapa sebopehong.Metabolomics e ikemiselitse ho lekanya bophara ba limolek'hule tse nyane ho latela maemo a 'mele kapa maemo a mafu.Mekhoa ea Metabolomics e oela ka lihlopha tse peli tse ikhethileng: metabolomics e sa rereloang, tlhahlobo e felletseng e reriloeng ea bahlahlobisisi bohle ba ka lekanyetsoang sampoleng ho kenyeletsoa le lik'hemik'hale tse sa tsejoeng tse sebelisang GC-MS/LC-MS, le metabolomics e lebisitsoeng, tekanyo ea lihlopha tse hlalositsoeng ka mokhoa oa lik'hemik'hale. metabolites e hlalositsoeng ka biochemical.
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Bulked Segregant analysis
Bulked segregant analysis (BSA) ke mokhoa o sebelisoang ho tsebahatsa matšoao a lefutso a amanang le phenotype.Ts'ebetso ea mantlha ea BSA e na le lihlopha tse peli tsa batho ba nang le phenotypes tse hanyetsanang haholo, ba kopanyang DNA ea batho bohle ho etsa bongata ba DNA, e khethollang tatellano e fapaneng lipakeng tsa matamo a mabeli.Mokhoa ona o sebelisitsoe haholo ho khetholla matšoao a lefutso a amanang haholo le liphatsa tsa lefutso tse lebisitsoeng ho liphatsa tsa lefutso tsa limela / tsa liphoofolo.
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DNA / RNA Sequencing - Nanopore Sequencer
Sequencing ea ONT ke molek'hule e le 'ngoe ea nako ea sebele ea theknoloji ea tatellano ea motlakase e thehiloeng ho nanopores, molao-motheo oa tatellano oa sethala ka seng o tšoana.DNA/RNA e nang le likhoele tse peli e tla tlama ho protheine ea nanoporous e kentsoeng ka har'a biofilm ebe e phutholoha tlas'a boetapele ba protheine ea motlakase, tlasa ts'ebetso ea phapang ea motlakase ho tsoa mahlakoreng ka bobeli a biofilm, likhoele tsa DNA/RNA li feta protheine ea kanal ea nanopore sebakeng se itseng. sekhahla.Ka lebaka la phapang ea lik'hemik'hale tsa metheo e fapaneng holim'a DNA / RNA strand, ha setsi se le seng kapa molek'hule ea DNA e feta mocha oa nanopore, e tla baka phetoho ea lipontšo tse fapaneng tsa motlakase.Ka ho lemoha le ho lumellana le lipontšo tsena, mefuta ea motheo e lumellanang e ka baloa, 'me ho lemoha nako ea sebele ea tatellano ho ka phethoa.
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DNA / RNA sequencing -PacBio Sequencer
Sethala sa tatellano sa PacBio ke sethala sa tatellano sa nako e telele, se tsejoang hape e le se seng sa mahlale a Third-Generation Sequencing (TGS).Theknoloji ea mantlha, molek'hule e le 'ngoe ea nako ea sebele(SMRT), e matlafatsa tlhahiso ea ho bala ka mashome a kilo-base ka bolelele.Motheong oa "Sequencing-by-Synthesis", qeto ea nucleotide e le 'ngoe e fihlelleha ka Zero-mode waveguide(ZMW), moo ho bonesitsoeng molumo o lekanyelitsoeng ka tlase (sebaka sa molecule synthesis).Ho feta moo, tatellano ea SMRT e qoba haholo tatellano e ikhethileng ea tatellano tsamaisong ea NGS, ka hore boholo ba mehato ea ntlafatso ea PCR ha e hlokehe ts'ebetsong ea kaho ea laeborari.
Sethala: Sequel II, Revio
