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Proteomics
Proteomics ya ƙunshi aikace-aikacen fasaha don ƙididdige yawan sunadaran sunadaran da ke gabatar da abun ciki na tantanin halitta, nama ko wata halitta.Ana amfani da fasahohin da ke da alaƙa da haɓakawa ta hanyoyi daban-daban don saitunan bincike daban-daban kamar gano alamomin bincike daban-daban, ƴan takara don samar da alluran rigakafi, fahimtar hanyoyin ƙwayoyin cuta, canjin yanayin magana don amsawa daban-daban sigina da fassarar hanyoyin gina jiki masu aiki a cikin cututtuka daban-daban.A halin yanzu, fasahar ƙididdige ƙididdigewa an raba su zuwa TMT, Label Free da dabarun ƙididdige DIA.
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Metabolomics
Metabolome shine samfurin ƙasa na genome kuma ya ƙunshi jimlar duk wani nau'in ƙananan ƙwayoyin ƙwayoyin cuta (metabolites) a cikin tantanin halitta, nama, ko kwayoyin halitta.Metabolomics na nufin auna faɗin ƙananan ƙananan ƙwayoyin cuta a cikin mahallin motsa jiki ko jihohin cututtuka.Hanyoyin metabolomics sun faɗi cikin ƙungiyoyi biyu daban-daban: metabolomics waɗanda ba a niyya ba, cikakken bincike da aka yi niyya na duk ƙididdigar ƙima a cikin samfurin ciki har da abubuwan da ba a sani ba ta amfani da GC-MS/LC-MS, da metabolomics da aka yi niyya, ma'aunin ƙayyadaddun ƙungiyoyin sinadarai da sinadarai. biochemically annotated metabolites.
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Babban bincike na Segregant
Binciken Segregant (BSA) wata dabara ce da aka yi amfani da ita don gano alamomin kwayoyin halitta masu alaƙa da sauri.Babban tsarin aiki na BSA ya ƙunshi zaɓin ƙungiyoyi biyu na mutane masu ƙima da ƙima, haɗa DNA na duk mutane don samar da mafi yawan DNA guda biyu, suna gano jerin bambance-bambance tsakanin tafkuna biyu.An yi amfani da wannan fasaha sosai wajen gano alamomin kwayoyin halitta masu alaƙa da alaƙa da kwayoyin da aka yi niyya a cikin kwayoyin halittar shuka/dabba.
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Tsarin DNA/RNA - Mai bin Nanopore
Sequencing na ONT wata kwayar halitta guda ɗaya ce ta fasaha ta siginar lantarki ta ainihin lokacin da ta dogara akan nanopores, ka'idar jerin kowane dandamali iri ɗaya ce.DNA/RNA mai ɗaure biyu zai ɗaure da sunadarin nanoporous da aka saka a cikin biofilm da kwancewa ƙarƙashin jagorancin furotin na motsa jiki, ƙarƙashin aikin bambancin ƙarfin lantarki daga ɓangarorin biofilm, sassan DNA / RNA suna wucewa ta cikin furotin tashar nanopore a wani takamaiman. ƙimar.Saboda bambance-bambancen abubuwan sinadarai na tushe daban-daban akan madaidaicin DNA/RNA, lokacin da tushe ɗaya ko kwayar DNA ta ratsa tashar nanopore, zai haifar da canjin siginar lantarki daban-daban.Ta hanyar ganowa da dacewa da waɗannan sigina, ana iya ƙididdige nau'ikan tushe daidai, kuma ana iya kammala gano ainihin lokacin.
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Tsarin DNA/RNA -PacBio Sequencer
PacBio dandali ne na jerin abubuwan da aka daɗe ana karantawa, wanda kuma aka sani da ɗayan fasahar Sequencing na ƙarni na uku (TGS).Babban fasahar, ainihin-kwale-kwale-kwale-kwale-kwale-kwale (SMRT), yana ba da ƙarfin ƙarni na karantawa tare da dubun-kwanan kilogiram cikin tsayi.A kan tushen "Sequencing-by-Synthesis", ƙudurin nucleotide guda ɗaya yana samuwa ta hanyar Zero-mode waveguide (ZMW), inda aka haskaka ƙayyadaddun ƙara kawai a ƙasa (shafin haɗin ƙwayoyin ƙwayoyin cuta).Bugu da ƙari, jeri na SMRT yana guje wa ƙayyadaddun ƙiyayya a cikin tsarin NGS, a cikin cewa yawancin matakan haɓaka PCR ba a buƙatar su a cikin aikin ginin ɗakin karatu.
Platform: Mabiyi II, Revio
