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IiProteomics
Iproteomics ibandakanya usetyenziso lwetekhnoloji yokulinganisa iiproteni zizonke ezikhoyo kwiseli, izicubu okanye into ephilayo.Ubuchwephesha obusekwe kwiproteomics busetyenziswa kwisakhono ezahlukeneyo kwizicwangciso ezahlukeneyo zophando ezifana nokufunyanwa kwamanqaku ahlukeneyo okuxilonga, abaviwa bokuvelisa isitofu sokugonya, ukuqonda iindlela ze-pathogenicity, ukuguqulwa kweepatheni zokubonisa ukuphendula kwimiqondiso eyahlukeneyo kunye nokutolikwa kweendlela zeprotheyini ezisebenzayo kwizifo ezahlukeneyo.Okwangoku, ubuchwephesha bobungakanani beproteomics bohlulwe ikakhulu kwi-TMT, iLabel Free kunye nezicwangciso zobungakanani be-DIA.
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IMetabolomics
I-metabolome yimveliso esezantsi ye-terminal ye-genome kwaye iqulethe i-complement epheleleyo yazo zonke ii-molecular-weight-weight-molecular (metabolites) kwiseli, izicubu, okanye i-organism.I-Metabolomics ijolise ekulinganiseni ububanzi obubanzi beemolekyuli ezincinci kumxholo we-physiological stimuli okanye izifo zesifo.Iindlela zeMetabolomics ziwela kumaqela amabini ahlukeneyo: i-metabolomics engajoliswanga, uhlahlelo olubanzi olujoliswe kulo lonke uhlalutyi olunokulinganiswa kwisampulu kubandakanywa iikhemikhali ezingaziwayo zisebenzisa i-GC-MS/LC-MS, kunye ne-metabolomics ekujoliswe kuyo, ukulinganiswa kwamaqela achaziweyo abonakaliswe ngokwekhemikhali kwaye i-biochemically annotated metabolites.
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Uhlalutyo lwe-Bulked Segregant
Uhlalutyo lwe-bulked segregant (BSA) bubuchule obusetyenziselwa ukuchonga ngokukhawuleza iimpawu zofuzo ezinxulumene nephenotype.Ukuhamba komsebenzi okuphambili kwe-BSA kuqulethe ukukhetha amaqela amabini abantu abaneephenotypes ezichasene kakhulu, ukudibanisa iDNA yabo bonke abantu ukwenza isambuku se-DNA, ukuchonga ulandelelwano olwahlukileyo phakathi kwamachibi amabini.Obu buchule busetyenziswe kakhulu ekuchongeni iimpawu zemfuza ezinxulunyaniswa ngamandla nemfuza ekujoliswe kuyo kwiigenome zezityalo/zezilwanyana.
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I-DNA / RNA Ukulandelelana - i-Nanopore Sequencer
Ukulandelelana kwe-ONT yi-molecule enye ye-real-time real-time signal sequencing technology esekelwe kwi-nanopores, umgaqo wokulandelelana kweqonga ngalinye liyafana.I-DNA/RNA enemisonto ephindwe kabini iya kubophelela kwiprotein ye-nanoporous efakwe kwi-biofilm kwaye ikhulule phantsi kwesikhokelo seprotein yemoto, phantsi kwesenzo somohluko wombane kumacala omabini e-biofilm, imitya ye-DNA/RNA idlula kwiprotein yesitishi se-nanopore kwindawo ethile. izinga.Ngenxa yokungafani kweempawu zeekhemikhali zeziseko ezahlukeneyo kwi-DNA / i-RNA strand, xa isiseko esisodwa okanye i-molecule ye-DNA idlula kumjelo we-nanopore, iya kubangela ukuguqulwa kweempawu zombane ezahlukeneyo.Ngokufumanisa kunye nokuhambelana nale miqondiso, iintlobo zesiseko ezihambelanayo zingabalwa, kwaye ukubonwa kwexesha langempela lokulandelelana kunokugqitywa.
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I-DNA / i-RNA yokulandelelana -I-PacBio Sequencer
I-platform yokulandelelanisa i-PacBio yiplatifomu yokulandelelanisa ixesha elide, eyaziwayo njengenye ye-Third-Generation Sequencing (TGS) teknoloji.Itekhnoloji engundoqo, i-molekyuli enye yexesha lokwenyani (SMRT), ixhobisa ukuveliswa kokufunda ngamashumi eekilo-isiseko ubude.Kwisiseko se "Sequencing-by-Synthesis", isisombululo se-nucleotide esisodwa siphunyezwa yi-Zero-mode waveguide (ZMW), apho kuphela umthamo oqingqiweyo osezantsi (indawo ye-molecule synthesis), iyakhanyiswa.Ukongeza, ulandelelwano lwe-SMRT luphepha kakhulu ulandelelwano oluthile kwinkqubo ye-NGS, ekubeni uninzi lwamanyathelo okukhulisa i-PCR awafuneki kwinkqubo yolwakhiwo lwethala leencwadi.
Iqonga: I-Sequel II, iRevio
