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Matenda a proteinomic
Proteomics imaphatikizapo kugwiritsa ntchito matekinoloje owerengera mapuloteni onse omwe amapezeka mu cell, minofu kapena chamoyo.Ukadaulo wopangidwa ndi mapuloteni amagwiritsidwa ntchito m'njira zosiyanasiyana pakufufuza kosiyanasiyana monga kuzindikira zolembera zosiyanasiyana, ofuna kupanga katemera, kumvetsetsa njira za pathogenicity, kusintha kamvekedwe ka mawu potengera zizindikiro zosiyanasiyana komanso kutanthauzira njira zamapuloteni zogwira ntchito m'matenda osiyanasiyana.Pakali pano, matekinoloje ochulukirachulukira a ma proteomics amagawidwa makamaka mu TMT, Label Free ndi njira za kuchuluka kwa DIA.
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Metabolomics
Metabolome ndi gawo lomaliza la jini ndipo limaphatikizana ndi mamolekyu otsika kwambiri (metabolites) mu selo, minofu, kapena chamoyo.Metabolomics cholinga chake ndi kuyeza kuchuluka kwa mamolekyu ang'onoang'ono malinga ndi momwe thupi limakhudzidwira kapena matenda.Njira za metabolomics zimagwera m'magulu awiri osiyana: metabolomics osalunjika, kusanthula kwatsatanetsatane kwa zowunikira zonse zomwe zingayesedwe muzachitsanzo kuphatikiza zosadziwika zamankhwala pogwiritsa ntchito GC-MS/LC-MS, ndi metabolomics omwe amayang'aniridwa, kuyeza kwa magulu omwe ali ndi mankhwala komanso ma metabolites a biochemically.
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Bulked Segregant kusanthula
Bulked segregant analysis (BSA) ndi njira yomwe imagwiritsidwa ntchito pozindikira mwachangu zolembera zamtundu wa phenotype.Kuyenda kwakukulu kwa BSA kumakhala ndi kusankha magulu awiri a anthu omwe ali ndi ma phenotypes otsutsana kwambiri, kuphatikiza DNA ya anthu onse kuti apange ma DNA awiri, kuzindikiritsa kutsatizana kosiyana pakati pa maiwe awiri.Njira imeneyi yakhala ikugwiritsidwa ntchito kwambiri pozindikira zizindikiro za majini zomwe zimagwirizanitsidwa kwambiri ndi majini omwe amawunikira mumagulu a zomera/zinyama.
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DNA / RNA Sequencing - Nanopore Sequencer
Kutsatizana kwa ONT ndi molekyu imodzi yeniyeni yeniyeni yotsatizana ndi teknoloji yotsatizana ndi nanopores, ndondomeko yotsatizana ya nsanja iliyonse ndi yofanana.DNA/RNA yokhala ndi mizere iwiri imamanga ku mapuloteni a nanoporous ophatikizidwa mu biofilm ndikumasuka motsogozedwa ndi mapuloteni amoto, chifukwa cha kusiyana kwa magetsi kuchokera mbali zonse za biofilm, zingwe za DNA/RNA zimadutsa mu protein ya nanopore panjira inayake. mlingo.Chifukwa cha kusiyana kwa mankhwala azitsulo zosiyanasiyana pa DNA / RNA strand, pamene maziko amodzi kapena molekyulu ya DNA imadutsa mumsewu wa nanopore, zidzachititsa kusintha kwa zizindikiro zosiyanasiyana zamagetsi.Pozindikira ndi kufananiza ndi zizindikirozi, mitundu yofananira yoyambira imatha kuwerengedwa, ndipo kuzindikira nthawi yeniyeni ya mndandanda kumatha kumalizidwa.
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DNA / RNA sequencing -PacBio Sequencer
PacBio sequencing platform ndi nsanja yowerengera nthawi yayitali, yomwe imadziwikanso kuti ndi imodzi mwaukadaulo wa Third-Generation Sequencing (TGS).Ukadaulo wapakatikati, single-molecule real-time(SMRT), umapatsa mphamvu zowerengera ndi makumi a kilo-base kutalika.Pansi pa "Sequencing-by-Synthesis", kusamvana kwa nucleotide imodzi kumatheka ndi Zero-mode waveguide(ZMW), pomwe voliyumu yocheperako pansi (malo a molecule synthesis), imawunikiridwa.Kuphatikiza apo, kutsatizana kwa SMRT kumapewa kukondera kwanthawi yayitali mu dongosolo la NGS, chifukwa njira zambiri zokulira za PCR sizofunikira pakumanga laibulale.
Platform: Sequel II, Revio
