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I-Proteomics
I-Proteomics ibandakanya ukusetshenziswa kobuchwepheshe bokulinganisa inani lamaprotheni ewonke akhona kuseli, izicubu noma into ephilayo.Ubuchwepheshe obusekelwe ku-Proteomics busetshenziswa ngezindlela ezihlukahlukene ezilungiselelweni ezihlukene zocwaningo njengokutholwa kwezimpawu zokuxilonga ezihlukahlukene, abantu abazokhiqiza imithi yokugoma, ukuqonda izindlela ze-pathogenicity, ukuguqulwa kwamaphethini okukhuluma ngokusabela kumasignali ahlukene kanye nokuchazwa kwezindlela zamaprotheni ezisebenzayo ezifweni ezihlukahlukene.Njengamanje, ubuchwepheshe be-quantitative proteomics buhlukaniswe ikakhulukazi ngamasu e-TMT, Amahhala Elebula kanye ne-DIA.
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I-Metabolomics
I-metabolome ingumkhiqizo ogcina phansi womfula we-genome futhi iqukethe isamba esiphelele sawo wonke ama-molecule anesisindo esiphansi (ama-metabolites) kuseli, izicubu, noma into ephilayo.I-Metabolomics ihlose ukukala ububanzi obubanzi bama-molecule amancane kumongo wezisusa ze-physiological noma izimo zezifo.Izindlela ze-Metabolomics ziwela emaqenjini amabili ahlukene: i-metabolomics engahlosiwe, ukuhlaziya okuhlosiwe okuhlosiwe kwazo zonke izihlaziyi ezingalinganiseka kusampula okuhlanganisa namakhemikhali angaziwa kusetshenziswa i-GC-MS/LC-MS, kanye ne-metabolomics eqondisiwe, ukukalwa kwamaqembu achaziwe aphawulwe ngamakhemikhali kanye ama-metabolites ane-biochemically annotated.
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Ukuhlaziywa kwe-Bulked Segregant
I-Bulked segregant analysis (BSA) iyindlela esetshenziswa ukuhlonza ngokushesha izimpawu zofuzo ezihlotshaniswa ne-phenotype.Ukuhamba komsebenzi okuyinhloko kwe-BSA kuqukethe ukukhetha amaqembu amabili abantu abane-phenotypes ephikisana kakhulu, okuhlanganisa i-DNA yabo bonke abantu ukuze bakhe inqwaba ye-DNA, okukhomba ukulandelana okuhlukene phakathi kwamachibi amabili.Le nqubo isetshenziswe kakhulu ekuhlonzeni izimpawu zofuzo ezihlotshaniswa kakhulu nezakhi zofuzo ezihlosiwe ku-genome yezitshalo/yezilwane.
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Ukulandelana kwe-DNA/RNA - I-Nanopore Sequencer
Ukulandelana kwe-ONT kuyi-molecule eyodwa yobuchwepheshe bokulandelanisa isignali kagesi yesikhathi sangempela ngokusekelwe kuma-nanopores, isimiso sokulandelana seplathifomu ngayinye siyefana.I-DNA/RNA enemicu emibili izobophezela kuphrotheni e-nanoporous eshumekwe ku-biofilm futhi ivuleke ngaphansi kokuhola kwe-motor protein, ngaphansi kwesenzo somehluko kagesi ezinhlangothini zombili ze-biofilm, imicu ye-DNA/RNA idlula kuphrotheni yesiteshi se-nanopore endaweni ethile. isilinganiso.Ngenxa yomehluko wezakhiwo zamakhemikhali ezisekelweni ezihlukene ku-DNA/RNA strand, lapho isisekelo esisodwa noma i-molecule ye-DNA idlula kumzila we-nanopore, izobangela ukushintshwa kwamasignali ahlukene kagesi.Ngokuthola nokuhambisana nalezi zimpawu, izinhlobo zesisekelo ezihambisanayo zingabalwa, futhi ukutholwa kwesikhathi sangempela sokulandelana kungaqedwa.
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Ukulandelana kwe-DNA/RNA -I-PacBio Sequencer
Iplathifomu yokulandelana ye-PacBio iyinkundla yokulandelana efunde isikhathi eside, eyaziwa nangokuthi obunye bobuchwepheshe bokulandelana kwesizukulwane sesithathu (TGS).Ubuchwepheshe obuyisisekelo, i-single-molecule real-time(SMRT), inika amandla ukukhiqizwa kokufunda ngamashumi ama-kilo-base ubude.Ngesisekelo se-"Sequencing-by-Synthesis", ukulungiswa kwe-nucleotide eyodwa kufinyelelwa yi-Zero-mode waveguide(ZMW), lapho kuphela ivolumu elinganiselwe ezansi(indawo yokuhlanganiswa kwe-molecule), ikhanyisiwe.Ukwengeza, ukulandelana kwe-SMRT kugwema kakhulu ukulandelana okuqondile ohlelweni lwe-NGS, ngokuthi izinyathelo eziningi zokukhulisa i-PCR azidingeki kunqubo yokwakha umtapo wolwazi.
I-Platform: I-Sequel II, i-Revio
