Cov khoom

Eukaryotic mRNA Sequencing-Illumina

mRNA sequencing enables profiling ntawm tag nrho cov mRNAs transcripted los ntawm hlwb nyob rau hauv tej yam kev mob.Nws yog lub tshuab muaj zog rau kev nthuav tawm cov noob qhia profile, cov qauv noob thiab cov txheej txheem molecular ntawm qee cov txheej txheem lom neeg.Txog niaj hnub no, mRNA sequencing tau dav siv hauv kev tshawb fawb, kev kuaj mob, kev tsim tshuaj, thiab lwm yam.

Platform: Illumina NovaSeq Platform

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Bioinformatics

Cov txiaj ntsig Demo

Qhov zoo

● Muaj Kev Paub Txog: Ntau tshaj 200,000 tus qauv tau ua tiav hauv BMK uas suav nrog ntau hom qauv, suav nrog kab lis kev cai ntawm tes, cov ntaub so ntswg, kua dej hauv lub cev, thiab lwm yam thiab ntau dua 7,000 mRNA-Seq tej yaam num raug kaw npog ntau yam kev tshawb fawb.

● Kev tswj hwm nruj nruj: Cov ntsiab lus tseem ceeb ntawm kev tswj hwm zoo los ntawm txhua kauj ruam suav nrog kev npaj ua qauv, kev npaj tsev qiv ntawv, kev sib txuas lus thiab bioinformatics yog nyob rau hauv kev saib xyuas zoo kom xa cov txiaj ntsig zoo.

● Ntau cov ntaub ntawv muaj nyob rau kev ua haujlwm annotation thiab kev tshawb fawb ntxiv kom ua tiav ntau hom kev tshawb fawb.

● Cov kev pab cuam tom qab muag: Cov kev pab cuam tom qab muag siv tau rau 3 lub hlis tom qab ua tiav qhov project, suav nrog cov phiaj xwm rov qab, kev daws teeb meem, cov txiaj ntsig Q&A, thiab lwm yam.

Sample Requirements thiab Delivery

Lub tsev qiv ntawv	Sequencing tswv yim	Cov ntaub ntawv pom zoo	Kev Tswj Xyuas Zoo
Poly A enriched	Illumina PE 150	6gb ib	Q30≥85%

Cov Qauv Xav Tau:

Nucleotides:

Conc. (ng / μl)

Tus nqi (μg)

Purity

Kev ncaj ncees

≥ 20

≥ 0.5

OD 260/280 = 1.7-2.5

OD 260/230 = 0.5-2.5

Txwv tsis pub lossis tsis muaj protein ntau lossis DNA kis tau pom ntawm gel.

Rau cov nroj tsuag: RIN≥6.5;

Rau tsiaj: RIN≥7.0;

5.0 ≥28S / 18S≥1.0;

txwv los yog tsis muaj qhov nce siab

Cov ntaub so ntswg: Nyhav (qhuav):≥1g
* Rau cov ntaub so ntswg me dua 5 mg, peb pom zoo kom xa flash khov (hauv kua nitrogen) cov ntaub so ntswg.

Cell ncua:Cell suav = 3 × 10⁶- 1 × 10⁷
* Peb pom zoo kom nkoj khov cell lysate.Yog hais tias lub xov tooj ntawm tes suav me dua 5 × 105.

Cov ntshav kuaj:Ntim ≥ 1 ml

Microorganism:Qhov hnyav ≥ 1 g

Pom zoo coj mus kuaj

Ntim: 2 ml centrifuge tube (Tin foil tsis pom zoo)

Sample labeling: Pab pawg + replicate xws li A1, A2, A3;B1, B2, B3....

Kev xa khoom:

Qhuav-ice: Cov qauv yuav tsum tau ntim rau hauv hnab thiab faus rau hauv cov dej khov qhuav.
RNAstable hlab: RNA cov qauv tuaj yeem qhuav hauv RNA stabilization raj (xws li RNAstable®) thiab xa mus rau hauv chav tsev kub.