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Genome-wide Association Analysis
Genome-wide Association txoj kev tshawb fawb (GWAS) lub hom phiaj ntawm kev txheeb xyuas cov noob caj noob ces (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype).GWAS txoj kev tshawb fawb soj ntsuam cov cim caj ces hla tag nrho cov genome ntawm cov neeg coob coob thiab kwv yees cov koom haum genotype-phenotype los ntawm kev txheeb cais ntawm cov pej xeem.Nws tau siv dav hauv kev tshawb fawb txog tib neeg cov kab mob thiab kev ua haujlwm gene mining ntawm cov yam ntxwv ntawm cov tsiaj lossis cov nroj tsuag.
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Ib leeg- nucleus RNA Sequencing
Kev nce qib hauv tib lub xov tooj ntawm tes tuav thiab ib tus neeg siv tsev qiv ntawv tsim cov txheej txheem sib txuas nrog kev sib txuas ua haujlwm siab tso cai rau cov kev tshawb fawb gene ntawm cov xov tooj ntawm tes.Nws ua kom muaj kev sib sib zog nqus thiab ua tiav kev tshuaj xyuas ntawm cov cell complex, nyob rau hauv uas nws feem ntau zam qhov npog ntawm lawv cov heterogeneity los ntawm kev noj nruab nrab ntawm tag nrho cov hlwb.
Txawm li cas los xij, qee lub hlwb tsis tsim nyog los ua rau hauv ib leeg-cell ncua kev kawm, yog li lwm cov qauv kev npaj - xav tau kev rho tawm nucleus los ntawm cov ntaub so ntswg, uas yog, nucleus raug rho tawm ncaj qha los ntawm cov ntaub so ntswg lossis cell thiab npaj rau hauv ib qho kev ncua rau ib leeg- cell sequencing.
BMK muab 10 × Genomics ChromiumTM raws li ib leeg-cell RNA sequencing kev pabcuam.Cov kev pabcuam no tau siv dav hauv kev tshawb fawb txog kev tshawb fawb txog kab mob, xws li kev tiv thaiv kab mob sib txawv, qog heterogeneity, kev loj hlob ntawm cov ntaub so ntswg, thiab lwm yam.
Spatial transcriptome nti: 10 × Genomics
Platform: Illumina NovaSeq Platform
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Nroj / Tsiaj Tag Nrho Genome Sequencing
Tag nrho genome re-sequencing, tseem hu ua WGS, ua rau kev nthuav tawm ntawm ob qho tib si thiab tsis tshua muaj kev hloov pauv ntawm tag nrho cov genome suav nrog Ib Leeg Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), thiab Copy Number Variation (CNV). ).SVs ua ib feem loj ntawm qhov hloov pauv ntau dua li SNPs thiab muaj kev cuam tshuam ntau dua rau cov genome, uas muaj kev cuam tshuam loj rau cov kab mob nyob.Kev nyeem ntawv ntev ntev tso cai rau kev txheeb xyuas qhov tseeb ntawm cov kab mob loj thiab cov kev hloov pauv nyuaj vim kev nyeem ntev ua rau nws yooj yim dua rau chromosomal hla cov cheeb tsam nyuaj xws li tandem rov ua dua, GC / AT-nplua nuj cheeb tsam, thiab hyper-variable cheeb tsam.
Platform: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Lub koom haum spatial ntawm cov hlwb ua lub luag haujlwm tseem ceeb hauv ntau yam txheej txheem lom neeg, xws li kev tiv thaiv kab mob, kev loj hlob ntawm embryo, thiab lwm yam. Spatial transcriptome sequencing, uas qhia txog noob caj noob ces profiling thaum khaws cov ntaub ntawv ntawm spatial txoj hauj lwm, tau muab kev pom zoo rau hauv cov ntaub so ntswg transcriptome.Nrog rau kev txhim kho thev naus laus zis, cov ntaub so ntswg ntshiab ultra-clear morphology thiab qhov sib txawv ntawm cov qauv ntawm spatial molecular qhia yuav tsum tau kawm nrog kev daws teeb meem siab dua.BMKGENE muab kev qhia dav dav, ib-nres spatial transcriptome sequencing kev pabcuam los ntawm cov qauv mus rau kev nkag siab txog kev lom neeg.
Spatial transcriptomics technologies txhawb cov kev xav tshiab hauv kev tshawb fawb sib txawv los ntawm kev daws cov noob qhia profile nrog cov ntsiab lus spatial hauv cov qauv heterogenous.
Spatial transcriptome nti: BMKMANU S1000
Platform: Illumina NovaSeq Platform
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10x Genomics Visium Spatial Transcriptome
Visium Spatial Gene Expression yog lub ntsiab lus tseem ceeb spatial transcriptome sequencing thev naus laus zis rau kev faib cov ntaub so ntswg raws li tag nrho mRNA.Daim ntawv qhia tag nrho cov ntawv sau nrog cov ntsiab lus morphological kom pom cov kev nkag siab tshiab rau hauv ib txwm muaj kev loj hlob, kab mob pathology, thiab kev tshawb fawb soj ntsuam kev txhais lus.BMKGENE muab kev qhia dav dav, ib-nres spatial transcriptome sequencing kev pabcuam los ntawm cov qauv mus rau kev nkag siab txog kev lom neeg.
Spatial transcriptomics technologies txhawb nqa cov kev xav tshiab hauv kev tshawb fawb sib txawv los ntawm kev daws cov noob qhia profile nrog cov ntsiab lus spatial hauv cov qauv heterogenous.
Spatial transcriptome nti: 10x Genomics Visium
Platform:Illumina NovaSeq Platform
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Full-Length mRNA Sequencing-Nanopore
RNA sequencing yog ib qho cuab yeej tseem ceeb rau kev txheeb xyuas cov ntaub ntawv sau tseg.Doubtlessly, ib txwm nyeem luv luv sequencing ua tiav ntau txoj kev loj hlob tseem ceeb hauv no.Txawm li cas los xij, nws feem ntau ntsib cov kev txwv nyob rau hauv tag nrho-ntev isoform identifications, quantification, PCR bias.
Nanopore sequencing distinguishes nws tus kheej los ntawm lwm cov sequencing platforms, nyob rau hauv hais tias cov nucleotides nyeem ncaj qha tsis muaj DNA synthesis thiab generates ntev nyeem ntawm kaum ntawm kilobases.Qhov no txhawb nqa kev nyeem ntawv ncaj qha hla cov ntawv teev lus ntev ntev thiab daws cov teeb meem hauv isoform-theem kev tshawb fawb.
Platform:Nanopor PromethION
Lub tsev qiv ntawv:cDNA-PCR
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Tag nrho-ntev mRNA sequencing - PacBio
De novfull-length transcriptome sequencing, tseem hu uaDe novIso-Seq siv qhov zoo ntawm PacBio sequencer hauv kev nyeem ntev, uas ua rau muaj kev sib luag ntawm tag nrho-ntev cDNA molecules yam tsis muaj kev so.Qhov no ua kom tsis txhob muaj qhov tsis raug tsim los ntawm cov ntawv sau ua ke thiab tsim cov unigene teeb nrog isoform-theem daws teeb meem.Cov txheej txheem unigene no muab cov ntaub ntawv muaj txiaj ntsig zoo li "reference genome" ntawm transcriptome-theem.Tsis tas li ntawd, kev sib txuas nrog cov ntaub ntawv txuas mus ntxiv, qhov kev pabcuam no txhawb nqa qhov tseeb ntawm isoform-theem qhia.
Platform: PacBio Sequel IILub tsev qiv ntawv: SMRT tswb tsev qiv ntawv -
Eukaryotic mRNA Sequencing-Illumina
mRNA sequencing enables profiling ntawm tag nrho cov mRNAs transcripted los ntawm hlwb nyob rau hauv tej yam kev mob.Nws yog lub tshuab muaj zog rau kev nthuav tawm cov noob qhia profile, cov qauv noob thiab cov txheej txheem molecular ntawm qee cov txheej txheem lom neeg.Txog niaj hnub no, mRNA sequencing tau dav siv hauv kev tshawb fawb, kev kuaj mob, kev tsim tshuaj, thiab lwm yam.
Platform: Illumina NovaSeq Platform
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Tsis siv neeg raws li mRNA Sequencing-Illumina
mRNA sequencing txais yuav cov txheej txheem txuas ntxiv tiam tom ntej (NGS) los ntes tus xa xov RNA (mRNA) daim ntawv Eukaryote ntawm lub sijhawm tshwj xeeb uas qee qhov tshwj xeeb ua haujlwm.Cov ntawv sau ntev tshaj plaws tau raug hu ua 'Unigene' thiab siv los ua cov ntaub ntawv siv rau kev soj ntsuam tom ntej, uas yog ib qho txiaj ntsig zoo los kawm txog cov txheej txheem molecular thiab kev tswj hwm kev sib txuas ntawm hom yam tsis siv.
Tom qab transcriptome cov ntaub ntawv los ua ke thiab unigene functional annotation
(1) SSR tsom xam, CDS kev kwv yees thiab cov qauv noob yuav raug ua ntej.
(2) Kev ua kom muaj nuj nqis ntawm unigene qhia hauv txhua tus qauv yuav ua.
(3) Sib txawv ntawm cov unigenes ntawm cov qauv (los yog pab pawg) yuav raug pom raws li unigene qhia
(4) Kev sib sau ua ke, cov lus piav qhia ua haujlwm thiab kev txheeb xyuas qhov sib txawv ntawm cov unigenes sib txawv yuav raug ua
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Ntev tsis-coding sequencing-Illumina
Ntev tsis-coding RNAs (lncRNAs) yog ib hom RNA molecules nrog ntev tshaj 200 nt, uas yog characterized los ntawm tsis tshua muaj coding peev xwm.LncRNA, raws li tus tswv cuab tseem ceeb hauv cov tsis-coding RNAs, feem ntau pom muaj nyob rau hauv nucleus thiab ntshav.Kev txhim kho hauv kev sib txuas thev naus laus zis thiab bioinformtics ua kom paub txog ntau yam tshiab lncRNAs thiab koom nrog cov haujlwm lom neeg.Cov ntaub ntawv pov thawj qhia tau hais tias lncRNA muaj kev koom tes hauv kev tswj hwm epigenetic, kev tswj hwm kev hloov pauv thiab kev tswj xyuas tom qab kev hloov pauv.
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Me RNA sequencing-Illumina
Me RNA yog hais txog ib chav kawm ntawm cov tsis-coding RNA molecules uas feem ntau tsawg dua 200nt nyob rau hauv ntev, suav nrog micro RNA (miRNA), me me cuam tshuam RNA (siRNA), thiab piwi-interacting RNA (piRNA).
MicroRNA (miRNA) yog ib chav kawm ntawm endogenous me RNA nrog ntev li ntawm 20-24nt, uas plays ntau yam tseem ceeb hauv kev tswj cov hlwb.miRNA koom nrog ntau cov txheej txheem ntawm lub neej uas nthuav tawm cov ntaub so ntswg - tshwj xeeb thiab theem - kev qhia tshwj xeeb thiab tau txais txiaj ntsig zoo hauv ntau hom.
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CircRNA sequencing-Illumina
CircRNAs (Circular RNAs) yog ib chav kawm ntawm cov tsis-coding RNA molecules uas luv ntawm 5′ kawg cap thiab 3′ kawg poly (A) tail.CircRNAs ua cov qauv ncig los ntawm covalent daim ntawv cog lus uas yog xwm txheej tiv thaiv RNA exonuclease digestion.circRNA plays lub luag haujlwm tseem ceeb hauv kev loj hlob thiab kev loj hlob ntawm cov kab mob thiab lawv cov tsis kam mus rau sab nraud ib puag ncig.
CircRNA muaj ntau txoj haujlwm, uas tuaj yeem sib tw khi miRNA, ua haujlwm tswj hwm kev ua haujlwm ntawm ceRNA, tswj cov noob qhia.Nyob rau hauv xyoo tas los no, nws kuj tau pom tias nws muaj feem cuam tshuam nrog kev tshwm sim thiab kev loj hlob ntawm cov kab mob, thiab muaj kev thov zoo rau kev cia siab rau cov kev taw qhia ntawm cov cim kab mob thiab lwm yam.
