16S/18S/ITS Amplicon Sequencing-NGS
Cov yam ntxwv ntawm kev pabcuam
● Lub platform txheeb xyuas cov ntaub ntawv: Illumina NovaSeq.
● Kev nthuav dav ntawm thaj chaw luv luv ntawm 16S, 18S thiab ITS, ntawm lwm cov hom phiaj nthuav dav.
● Kev xaiv yooj ywm ntawm amplicon.
● Kev paub dhau los ntawm qhov project nrog ntau lub hom phiaj amplification.
Cov Kev Pabcuam Zoo
●Tsis muaj kev cais tawm:Kev txheeb xyuas sai ntawm cov kab mob me me hauv cov qauv ib puag ncig.
●Kev daws teeb meem siab: Hauv cov khoom tsawg-ntau hauv cov qauv ib puag ncig.
●Siv Tau DavKev tshawb fawb txog cov kab mob sib txawv.
●Kev Tshawb Fawb Txog Bioinformatics Uas Muaj Kev Paub Tag NrhoCov pob khoom QIIME2 tshiab kawg (kev nkag siab txog kev ntsuas ntau yam hauv kev ua neej ntawm cov kab mob me me) nrog ntau yam kev tshuaj xyuas ntawm cov ntaub ntawv, cov lus piav qhia, OTU/ASV.
●Kev Paub Ntau YamBMKGENE muaj 150 txhiab qhov project amplicon sequencing uas tau ua txhua xyoo, thiab muaj ntau tshaj kaum xyoo ntawm kev paub dhau los, pab pawg neeg tshuaj xyuas uas muaj kev txawj ntse heev, cov ntsiab lus dav dav, thiab kev txhawb nqa tom qab muag zoo heev.
Cov Lus Qhia Txog Kev Pabcuam
| Tsev qiv ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv pom zoo |
| Amplicon | Illumina PE250 | 50/100/300K cim npe (Nyeem Khub) |
Cov Kev Cai rau Kev Pabcuam
| Kev Ntsuas (ng/µL) | Tag nrho cov nyiaj (ng) | Ntim (µL) |
| ≥1 | ≥200 | ≥20 |
● Av/sludge: 1-2g
● Cov ntsiab lus ntawm cov hnyuv-tsiaj: 0.5-2g
● Cov khoom hauv plab hnyuv - kab: 0.1-0.25g
● Nto ntawm tsob nroj (cov av nplua nuj): 0.1-0.5g
● Cov kua zaub uas muaj cov av nplaum ntau ntxiv rau hauv cov kua zaub fermentation): 0.1-0.5g
● Cov quav (cov tsiaj loj): 0.5-2g
● Cov quav (nas): 3-5 cov noob
● Cov kua ntxuav lub ntsws alveolar: daim ntawv lim dej
● Daim ntaub so qhov chaw mos: 5-6 daim ntaub so
● Daim ntaub so tawv nqaij/qhov chaw mos/cov qaub ncaug/cov nqaij mos mos hauv qhov ncauj/cov ntaub so caj pas/cov ntaub so qhov quav: 2-3 daim ntaub so
● Cov kab mob me me ntawm qhov chaw: daim ntawv lim dej
● Lub cev dej/cua/biofilm: daim ntawv lim dej
● Cov tshuaj Endophytes: 1-2g
● Cov Plaque Hniav: 0.5-1g
Kev Ua Haujlwm Pabcuam
Kev xa khoom qauv
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Muaj xws li cov kev tshuaj xyuas hauv qab no:
- Kev tswj xyuas cov ntaub ntawv raw zoo
- OTU clustering / De-suab nrov (ASV)
- Cov lus piav qhia ntawm OTU
- Kev tshuaj xyuas qhov sib txawv ntawm Alpha: ntau cov ntsuas, suav nrog Shannon, Simpson thiab ACE.
- Kev tshuaj xyuas ntau haiv neeg ntawm Beta
- Kev tshuaj xyuas ntawm pawg neeg sib txawv
- Kev tshuaj xyuas kev sib raug zoo: ntawm cov yam ntxwv ib puag ncig thiab OUT cov khoom sib xyaw thiab ntau haiv neeg
- Kev kwv yees txog kev ua haujlwm ntawm 16S gene
Histogram ntawm kev faib tawm ntawm taxonomic
Daim ntawv qhia kub ntawm kev faib tawm ntawm cov pawg neeg muaj ntau yam
Kev tshuaj xyuas ntau haiv neeg ntawm Alpha: qhov nkhaus tsis tshua muaj
Kev tshuaj xyuas ntau haiv neeg beta: NMDS
Kev tshuaj xyuas ntawm pawg neeg sib txawv: Kev tshawb pom LEFSE biomarker
Tshawb nrhiav kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam amplicon sequencing nrog Illumina los ntawm kev sau cov ntawv tshaj tawm.
Dong, C. et al. (2022) 'Kev Sib Dhos, Core Microbiota, thiab Kev Ua Haujlwm ntawm Rhizosphere Av thiab Bark Microbiota hauv Eucommia ulmoides', Frontiers in Microbiology, 13. doi: 10.3389/FMICB.2022.855317/FULL.
Li, Y. et al. (2023) 'Kev hloov pauv kab mob sib xyaw ua ke rau kev kho mob Gardnerella vaginalis-induced bacterial vaginosis hauv nas', Microbiome, 11(1), pp. 1–14. doi: 10.1186/s40168-023-01497-y
Yang, J., Fu, Y. thiab Liu, H. (2022) 'Cov kab mob me me ntawm cov hmoov av hauv huab cua uas tau sau thaum lub sijhawm sim ua kom lub neej rov qab zoo li qub hauv av "Lunar Palace 365"', Environmental Microbiomes, 17(1), pp. 1–20. doi: 10.1186/S40793-022-00399-0/FIGURES/8.
Yin, S. et al. (2022) 'Kev muaj ntau yam noob caj noob ces uas cuam tshuam nrog kev hloov pauv nitrogen uas tswj kev poob nitrogen hauv kev ua composting', Bioresource Technology, 361, p. 127678. doi: 10.1016/J.BIORTECH.2022.127678.
