Sib piv Genomics
Kev Pabcuam Zoo
● Kev txheeb xyuas cov pob khoom, uas muaj yim qhov kev soj ntsuam uas xav tau tshaj plaws
● Kev ntseeg siab hauv kev tshuaj xyuas nrog cov ncauj lus kom ntxaws thiab nkag siab yooj yim ntawm cov txiaj ntsig
● Zoo-tsim npaj-rau-tshaj tawm cov duab
● Pab pawg neeg paub txog bioinformatics tau ua tiav ntau yam kev xav tau ntawm tus kheej
● Lub sijhawm tig rov qab luv dua nrog qhov tseeb dua hauv kev tshuaj xyuas
● Muaj kev paub dhau los nrog ntau dua 90 qhov xwm txheej ua tiav nrog cov ntawv tshaj tawm cuam tshuam ntau dua 900
Service Specifications
| Kwv yees lub sijhawm tig rov qab | Tus naj npawb ntawm hom | Kev tshuaj xyuas |
| 30 hnub ua haujlwm | 6–12 | Gene family clustering Gene tsev neeg expansion thiab contraction Phylogenetic ntoo tsim Divergence lub sij hawm kwv yees (Fossil calibration yuav tsum tau) LTR tso lub sij hawm (Rau cov nroj tsuag) Tag nrho genome duplication (Rau cov nroj tsuag) Xaiv lub siab Synteny tsom xam |
Bioinformatics tsom xam
● Gene tsev neeg
● Phylogenetics
● Lub sijhawm sib txawv
● Xaiv lub siab
● Synteny tsom xam
Sample Requirements thiab Delivery
Cov Qauv Xav Tau:
Cov ntaub so ntswg los yog DNA rau genome sequencing thiab sib dhos
Rau Tissue
| Hom | Cov ntaub so ntswg | Kev soj ntsuam | PacBio CCS |
| Tsiaj | Visceral ntaub so ntswg | 0.5 ~ 1g | Yog '> 3.5 g |
| Cov nqaij mos | |||
| ≥ 5.0g | |||
| ≥ 5.0ml | |||
| Mammalian ntshav | |||
| ≥ 0.5 mL | |||
| Nqaij qaib / Ntses ntshav | |||
| Nroj tsuag | Nplooj tshiab | 1 ~ 2g | ≥ 5.0g |
| Nplooj / qia | 1 ~ 2g | ≥ 10.0g | |
| Hauv paus / noob | 1 ~ 2g | ≥ 20.0g | |
| Cells | Cultivated cell | - | ≥ 1 x 108 |
Cov ntaub ntawv
genome sequence files (.fasta) thiab annotation cov ntaub ntawv (.gff3) ntawm cov tsiaj uas ze ze
Kev Pabcuam Ua Haujlwm Flow
Kev sim tsim
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
*Cov txiaj ntsig tau pom ntawm no yog tag nrho los ntawm genomes luam tawm nrog Biomarker Technologies
1.LTR insert lub sij hawm kwv yees: Daim duab qhia ib tug tshwj xeeb bimodal tis nyob rau hauv LTR-RTs insertion lub sij hawm nyob rau hauv Weining rye genome, piv rau lwm hom.Qhov siab tshaj plaws tsis ntev los no tau tshwm sim nyob ib ncig ntawm 0.5 lab xyoo dhau los.
Li Guang et al.,Nature Genetics, 2021 ib
2.Phylogeny thiab gene tsev neeg tsom xam ntawm chayote (Sechium edule): Los ntawm kev tshuaj xyuas chayote thiab lwm yam 13 yam muaj feem xyuam hauv tsev neeg, Chayote tau pom tias muaj feem cuam tshuam nrog nab gourd (Trichosanthes anguina).Chayote muab los ntawm nab gourd nyob ib ncig ntawm 27-45 Mya thiab tag nrho genome duplication (WGD) tau pom nyob rau hauv chayote hauv 25 ± 4 Mya, uas yog qhov thib peb WGD tshwm sim hauv cucuibitaceae.
Fu A et al.,Kev Tshawb Fawb Txog Kev Tshawb Fawb, 2021 ib
3.Synteny tsom xam: Qee cov noob muaj feem xyuam rau phytohormones hauv kev loj hlob txiv hmab txiv ntoo tau pom nyob rau hauv chayote, nab gourd thiab squash.Kev sib raug zoo ntawm chayote thiab squash yog me ntsis siab dua li ntawm chayote thiab nab gourd.
Fu A et al.,Kev Tshawb Fawb Txog Kev Tshawb Fawb, 2021 ib
4.Gene family analysis: KEGG enrichment on gene family expansion and contraction in G.thurberi thiab G.davidsonii genomes qhia tias steroid biosynthesis thiab brassinosteroid biosynthesis ntsig txog cov noob tau nthuav dav.
Yang Z et al.,BMC Biology, 2021 ib
5.Txhua qhov kev tshuaj ntsuam genome duplication: 4DTV thiab Ks kev faib tawm kev tsom xam pom tag nrho genome duplication tshwm sim.Peaks ntawm intraspecies qhia duplication txheej xwm.Peaks ntawm interspecies qhia speciation txheej xwm.Qhov kev tshuaj ntsuam qhia tau hais tias muab piv nrog rau lwm yam peb hom tsiaj, O. europaea tau dhau los ntawm cov noob qoob loo loj dua tsis ntev los no.
Rao G et al.,Kev Tshawb Fawb Txog Kev Tshawb Fawb, 2021 ib
BMK Cas
Rose tsis muaj prickle: genomic insights txuas rau ya raws adaptation
Luam tawm: National Science Review, 2021 ib
Sequence Strategy:
'Basy'sThornless' (R.Wichurainan) genome:
Kwv yees li.93 X PacBio + ca.90 X Nanopore + 267 X Illumina
Cov txiaj ntsig tseem ceeb
1.High zoo R.wichuraiana genome tau tsim los siv cov txheej txheem nyeem ntev ntev, uas ua rau muaj kev sib dhos ntawm 530.07 Mb (Kev kwv yees genome loj yog kwv yees li 525.9 Mb los ntawm kev ntws cytometry thiab 525.5 los ntawm genome survey； Heterozygosity nyob ib ncig ntawm 525.9 Mb.BUSCO kwv yees tus qhab nia yog 93.9%.Muab piv nrog "Old blush" (haploOB), qhov zoo thiab ua tiav ntawm cov genome no tau lees paub los ntawm qhov tseeb ntawm lub hauv paus ib leeg thiab LTR sib dhos Performance index (LAI = 20.03).R.wichuraiana genome muaj 32,674 protein coding noob.
2.Multi-omics kev sib koom ua ke, suav nrog kev sib piv genomics, transcriptomics, QTL kev tshuaj ntsuam ntawm cov noob caj noob ces, qhia txog qhov tseem ceeb ntawm R. wichuraiana thiab Rosa chinensis.Tsis tas li ntawd, kev qhia txawv ntawm cov noob muaj feem xyuam hauv QTL yuav muaj feem cuam tshuam nrog qia prickle qauv.
Kev sib piv genomics anaysis ntawm Basye; s Thornless thiab Rosa chinensis nrog rau kev tsom xam synteny, gene family cluster, expansion and contraction analysis, nthuav tawm ntau qhov kev hloov pauv, uas muaj feem xyuam rau qhov tseem ceeb hauv roses.Qhov tshwj xeeb nthuav dav hauv NAC thiab FAR1 / FRS gene tsev neeg muaj feem cuam tshuam nrog kev tiv thaiv rau qhov chaw dub.
Kev sib piv genomics tsom xam ntawm BT thiab haploOB genomes.
Zhong, M., et al."Rose tsis muaj prickle: genomic insights txuas rau ya raws adaptation"National Science Review, 2021; nwb 092.
