Nroj / Tsiaj Tag Nrho Genome Sequencing
1. Kev Pabcuam Zoo
Kev paub dav dav hauv genome sequencing rau ntau dua 1000 hom.
Ntau tshaj 800 qhov xwm txheej luam tawm nrog qhov cuam tshuam ntau dua ntawm ntau dua 4000.
Kev txheeb xyuas bioinformatics ntawm kev sib txawv hu thiab kev tsom xam ua haujlwm.
2. Kev Pabcuam Specifications
| Platform | Lub tsev qiv ntawv | Pom zoo seq qhov tob | |
| Illumina | PEB 150 | Rau SNP, InDel hu ≥ 10x Rau SV, CNY hu ≥ 30x
| |
| Nanopor
| 8kb ib | Rau SV, CNY hu ≥ 20x | |
| Pacbio | CCS | 15kb ib | Rau SNP, InDel, SV, CNY hu ≥ 10x |
3. Cov qauv yuav tsum tau ua
| Platform | Conc.(ng/μL)
| Tus nqi (ng)
| Purity
| Agarose gel
| ||
| OB 260/280 | OB 260/230 | 1. Clear lub ntsiab band uas tsis muaj lossis txwvdegradation pom ntawm gel. 2. Tsis muaj lossis txwv tsis pub muaj RNA lossis cov protein ntau
| ||||
| Illumina | ≥1 | ≥30
| - | - | ||
| Nanopor
| ≥30 | Nyob ntawm cov ntaub ntawv tawm los 10 μg / cell | 1.7-2.2 | ≥1.5 | ||
| Pacbio | CCS | ≥50 | 1.7-2.2 | 1.8-2.5 Nws | ||
4. Bioinformation Analysis
5. Kev Pabcuam Ua Haujlwm Zoo
Qauv xa tuaj
DNA rho tawm
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev xa cov ntaub ntawv
1) Statistics ntawm Genome Mapping
Table 1 Statistics of map result
Daim duab 1 Kev faib tawm ntawm qhov loj me thiab nyeem qhov kev pab cuam.
2) Variate Detection
Daim duab 2 Statistics thiab annotation ntawm SNP / INDEL / SV ntawm Cov Qauv
Daim duab 3 Genome-wide faib ntawm vatiations
3) Muaj nuj nqi annotation ntawm variations
| 2019 | Ntuj kev sib txuas lus | Tag nrho-genome resequencing qhia Brassica napus keeb kwm thiab caj ces loci koom nrog hauv nws txoj kev txhim kho |
| 2020 | PNAS | Keeb kwm evolutionary thiab domestication keeb kwm ntawm goldfish (Carassius auratus) |
| 2021 | Plant Biotechnology Journal | Kev siv genomes ntawm ob hom jute cultivated |
| 2021 | Plant Biotechnology Journal | Genomic kos npe ntawm zaub thiab oilseed allopolyploid Brassicajuncea thiab genetic loci tswj cov tsub zuj zuj ntawm glucosinolates |
| 2019 | Molecular Cog | Tag nrho-genome resequencing ntawm ib tug thoob ntiaj teb sau ntawm rapeseed accessions qhia genetic hauv paus ntawm lawv ecotype divergence |
| 2022 | Kev Tshawb Fawb Txog Kev Tshawb Fawb | Kev tshuaj ntsuam genome-wide koom haum muab cov kev nkag siab molecular rau hauv kev hloov pauv ntawm cov noob txiv ntoo loj |
| 2021 | Phau ntawv Journal of Experimental Botany | Genome-wide Association txoj kev tshawb fawb txheeb xyuas qhov sib txawv ntawm GhSAD1 hais txog kev ua siab ntev ntev hauv paj rwb |
| 2021 | Phau ntawv Journal of Experimental Botany | Genome-wide koom haum txoj kev kawm thiab kev sib piv transcriptome qhia tshiab QTL thiab cov neeg sib tw cov noob uas tswj cov nplooj loj hauv rapeseed |
