Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
Kev Pabcuam Nta
● Kev pabcuam yuav tsum tau kuaj cov ntaub so ntswg, es tsis txhob rho tawm cov nucleic acids, kom txuag tau DNA-protein kev sib cuam tshuam.
● ATAC txoj kev suav nrog cov ntaub so ntswg dissociation, ua raws li kev rho tawm nuclei, Tn5 kho thiab purification, PCR amplification, xaiv qhov loj me thiab sib txuas.
● Sequencing hauv Illumina NovaSeq
Kev Pabcuam Zoo
●High Sensitivity:qis pib ntawm tes kom muaj nuj nqis txaus rau kev npaj tsev qiv ntawv thiab ua kom tiav.
●Cov ntaub ntawv zoo heev: ib txhij nthuav tawm cov chaw genomic ntawm qhib chromatin thiab cov txheej txheem tswj kev ua haujlwm xws li cov chaw sib txuas lus sib txuas.
●Kev sim ua kom rov ua tau zoo: technical replicates qhia zoo heev repeatability.
●Kev txawj ntse: nrog ntau pua ntawm ATAC sequencing tej yaam num ua tiav, BMKGENE coj ntau tshaj kaum xyoo ntawm kev paub, pab pawg neeg soj ntsuam zoo heev, cov ntsiab lus nthuav dav, thiab kev txhawb nqa tom qab muag zoo heev.
●Muaj peev xwm koom nrog Transcriptomics Analysis: tso cai rau kev sib koom ua ke ntawm ATAC seq nrog lwm cov ntaub ntawv omics xws li RNA-seq.
● Kev Tshawb Fawb Txog Kev Tshawb Fawb Txog Bioinformatic: ua kom tsis tsuas yog kev txheeb xyuas ntawm thaj chaw qhib ntawm chromatin thiab lawv cov haujlwm sib xws (promoters, UTs, exons, introns) tab sis kuj tseem muaj kev tshuaj xyuas qhov sib txawv ntawm chromatin qhib thaj tsam ntawm cov qauv.
Service Specifications
| Lub tsev qiv ntawv | Sequence Strategy | Pom zoo cov ntaub ntawv tso zis | Kev tswj kom zoo |
| ATAC-sib | Illumina PE 150 | > 20M nyeem Nyob ntawm qhov loj ntawm genome (Tib neeg: 50 M nyeem) | Q30≥85% |
Cov Qauv Xav Tau
Hom qauv: cov ntaub so ntswg, nyob los yog khov hlwb, ntshav
● Cell xov tooj: ≥ 106hlwb
● Cov ntaub so ntswg hnyav: ≥ 200mg cov ntaub so ntswg tshiab
● Ntshav: ≥ 4 mL
Kev Pabcuam Ua Haujlwm Flow
Kev sim tsim
Qauv xa tuaj
DNA rho tawm
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Xws li Kev Tshawb Fawb Hauv qab no:
● Raw cov ntaub ntawv zoo tswj;
● Peak hu raws li daim ntawv qhia rau siv genome;
● Gene annotation ntawm qhov chaw siab tshaj;
● Motif Analysis: txheeb xyuas qhov chaw sib txuas lus (TFBS);
● Differential Peak Analysis thiab annotation.
1.Heatmap ntawm ATAC nyeem kev faib tawm ntawm TSS thiab cov cheeb tsam uas nyob ib sab (± 3 kb)
2.Kev faib tawm ntawm thaj chaw qhib chromatin hauv cov khoom sib txawv ntawm cov genome
3.Differential ncov hu ntawm pab pawg
Tshawb nrhiav cov kev tshawb fawb nce qib los ntawm BMKGene's ATAC sequencing cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm.
Fu, A.ua al.(2023) 'Telomere-to-telomere genome los ua ke ntawm iab melon (Momordica charantia L. var. abbreviata Ser.) qhia cov txiv hmab txiv ntoo loj hlob, muaj pes tsawg leeg thiab ripening genetic yam ntxwv',Kev Tshawb Fawb Txog Kev Tshawb Fawb, 10(1). doi: 10.1093/HR/UHAC228.
Gong, B.ua al.(2023) 'Epigenetic thiab transcriptional activation ntawm secretory kinase FAM20C ua ib qho oncogene hauv glioma',Phau ntawv Journal of Genetics thiab Genomics, 50(6), pp. 422–433. doi: 10.1016/J.JGG.2023.01.008.
Nws, Y.ua al.(2023) 'Butyrate thim rov qab ferroptosis tsis kam nyob rau hauv mob qog noj ntshav los ntawm inducing c-Fos-dependent xCT suppression',Redox Biology, 65 , ib. 102822. doi: 10.1016/J.REDOX.2023.102822.
