Kev Tshawb Fawb Txog Metagenomic -NGS
Ib qho metagenome yog ib qho kev sib sau ua ke ntawm tag nrho cov khoom siv caj ces ntawm ib lub zej zog sib xyaw ntawm cov kab mob, xws li ib puag ncig thiab tib neeg metagenomes. Nws muaj cov genomes ntawm ob qho tib si cog tau thiab tsis cog tau. Shotgun metagenomic sequencing nrog NGS ua rau kev kawm txog cov toj roob hauv pes genomic nyuaj no uas tau muab tso rau hauv cov qauv ib puag ncig los ntawm kev muab ntau dua li kev txheeb xyuas taxonomic, muab kev nkag siab tob txog ntau haiv neeg, kev nplua nuj dynamics, thiab cov qauv pej xeem nyuaj. Dhau li ntawm kev tshawb fawb taxonomic, shotgun metagenomics kuj muab kev pom genomics ua haujlwm, ua rau kev tshawb nrhiav cov noob encoded thiab lawv lub luag haujlwm hauv cov txheej txheem ecological. Thaum kawg, kev tsim cov tes hauj lwm sib raug zoo ntawm cov ntsiab lus genetic thiab cov yam ntxwv ib puag ncig pab txhawb rau kev nkag siab holistic ntawm kev sib cuam tshuam nyuaj ntawm cov zej zog microbial thiab lawv keeb kwm yav dhau los ecological. Hauv kev xaus, metagenomic sequencing sawv cev rau lub cuab yeej tseem ceeb rau kev daws cov genomic intricacies ntawm ntau haiv neeg microbial, qhia txog kev sib raug zoo ntawm ntau yam genetics thiab ecology hauv cov ecosystem nyuaj no.
Cov platforms: Illumina NovaSeq thiab DNBSEQ-T7
Cov Kev Pabcuam Zoo
●Txoj Kev Cais Tawm thiab Tsis Muaj Kev Cog Qoob Loo Rau Kev Tshawb Fawb Txog Lub Zej Zog MicrobialPab kom muaj kev txheeb xyuas cov khoom siv caj ces los ntawm cov kab mob uas tsis tuaj yeem cog tau.
●Kev daws teeb meem siabTshawb nrhiav cov tsiaj txhu uas muaj tsawg hauv cov qauv ib puag ncig.
●Kev Tshuaj Xyuas Txog Bioinformatics Uas Muaj Kev Paub Tag Nrho:Tsis yog tsuas yog tsom mus rau qhov sib txawv ntawm taxonomic xwb tab sis kuj tseem tsom mus rau qhov sib txawv ntawm kev ua haujlwm ntawm lub zej zog.
●Kev Paub Ntau Yam:Peb pab neeg muaj keeb kwm ua tiav ntau qhov project metagenomics hauv ntau qhov chaw tshawb fawb thiab ua tiav ntau dua 200,000 qhov qauv, peb pab neeg coj ntau yam kev paub dhau los rau txhua qhov project.
Cov Lus Qhia Txog Kev Pabcuam
| Lub platform txheeb xyuas cov ntaub ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv pom zoo | Kev tswj xyuas zoo |
| Illumina NovaSeq los yog DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Cov Kev Cai rau Kev Pabcuam
| Kev Ntsuas (ng/µL) | Tag nrho cov nyiaj (ng) | Ntim (µL) |
| ≥1 | ≥30 | ≥20 |
● Av/sludge: 2-3g
● Cov ntsiab lus ntawm cov hnyuv-tsiaj: 0.5-2g
● Cov khoom hauv plab hnyuv - kab: 0.1-0.25g
● Nto ntawm tsob nroj (cov av nplua nuj): 0.5-1g
● Cov kua zaub uas muaj cov av nplaum ntau ntxiv rau hauv cov kua zaub fermentation): 0.2-0.5g
● Cov quav (cov tsiaj loj): 0.5-2g
● Cov quav (nas): 3-5 cov noob
● Cov kua ntxuav lub ntsws alveolar: daim ntawv lim dej
● Daim ntaub so qhov chaw mos: 5-6 daim ntaub so
● Daim ntaub so tawv nqaij/qhov chaw mos/cov qaub ncaug/cov nqaij mos mos hauv qhov ncauj/cov ntaub so caj pas/cov ntaub so qhov quav: 2-3 daim ntaub so
● Cov kab mob me me ntawm qhov chaw: 5-6 daim ntaub so ntswg
● Lub cev dej/cua/biofilm: daim ntawv lim dej
● Cov Endophytes: 2-3g
● Cov Plaque Hniav: 0.5-1g
Kev Ua Haujlwm Pabcuam
Kev xa khoom qauv
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Muaj xws li cov kev tshuaj xyuas hauv qab no:
● Kev tswj xyuas cov ntaub ntawv zoo ntawm kev txheeb xyuas cov ntaub ntawv
● Kev sib sau ua ke ntawm Metagenome thiab kev kwv yees txog cov noob caj noob ces
● Kev piav qhia txog noob caj noob ces
● Kev tshuaj xyuas ntau haiv neeg ntawm cov alpha taxonomic
● Kev tshuaj xyuas kev ua haujlwm ntawm lub zej zog: kev ua haujlwm ntawm lub cev, kev zom zaub mov, kev tiv thaiv tshuaj tua kab mob
● Kev tshuaj xyuas txog ob qho tib si kev ua haujlwm thiab kev faib tawm ntawm cov tsiaj txhu:
Kev tshuaj xyuas ntau haiv neeg ntawm Beta
Kev tshuaj xyuas ntawm pawg neeg sib txawv
Kev tshuaj xyuas kev sib raug zoo: ntawm cov yam ntxwv ib puag ncig thiab OUT cov khoom sib xyaw thiab ntau haiv neeg
Kev tshuaj xyuas kev ua haujlwm: CARD kev tiv thaiv tshuaj tua kab mob
Kev tshuaj xyuas sib txawv ntawm KEGG metabolic pathways: heatmap ntawm cov pathways tseem ceeb
Alpha ntau haiv neeg ntawm kev faib tawm taxonomic: ACE index
Beta diversity ntawm taxonomic faib: PCoA
Tshawb nrhiav cov kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam metagenome sequencing nrog Illumina los ntawm kev sau cov ntawv tshaj tawm.
Hai, Q. et al. (2023) 'Kev tshuaj xyuas Metagenomic thiab Metabolomic ntawm kev hloov pauv hauv cov ntsiab lus ntawm cov hnyuv ntawm cov ntses rainbow trout (Oncorhynchus mykiss) uas muaj tus kab mob hematopoietic necrosis virus ntawm cov dej sib txawv ntawm cov dej kab lis kev cai',Cov ciam teb hauv Microbiology, 14, nplooj 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Cov zej zog kab mob me me, cov noob tiv taus, thiab kev pheej hmoo tiv taus hauv cov pas dej hauv nroog ntawm ntau lub xeev trophic sib txawv: Kev sib txuas sab hauv thiab kev cuam tshuam sab nraud',Phau Ntawv Xov Xwm Txog Kev Txhim Kho Cov Khoom Phom Sij,,9 ,ua. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Kev Tshawb Fawb Txog Metagenomic Qhia Txog Qhov Txawv ntawm Cov Khoom Siv thiab Kev Ua Haujlwm Ntawm Cov Kab Mob Me Me Uas Muaj Kua Thiab Cov Khoom Uas Muaj Khoom Ntawm Cov Rumen Yaj',Cov ciam teb hauv Microbiology, 13, nplooj ntawv 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Cov kab mob microbiota uas muaj los ntawm npua uas rog dhau lawm rov ua kom cov carnitine metabolism zoo dua los txhawb cov leeg nqaij fatty acid deposition hauv cov npua DLY uas tsis muaj roj ntau',Kev Tsim Kho Tshiab, 4(5), p. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Kev nkag siab txog Metagenomic txog kev pheej hmoo ntawm cov yas uas sawv cev rau cov khoom siv bio/non-degradable thiab cov khib nyiab uas tsis yog yas nyob rau sab saud thiab qis dua ntawm Haihe Estuary, Suav teb',Kev Tshawb Fawb Txog Ib Puag Ncig Tag NrhoIb., 887, p. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
