Kev Txheeb Xyuas CircRNA-Illumina
Cov yam ntxwv
● Kev tshem tawm rRNA ua raws li kev npaj cov tsev qiv ntawv qhia kev taw qhia, ua kom cov ntaub ntawv sequencing tshwj xeeb rau cov strand.
● Kev ua haujlwm bioinformatic ua rau muaj kev kwv yees circRNA thiab kev ntsuas kev qhia tawm.
Cov Kev Pabcuam Zoo
●Kev Paub Ntau YamPeb tau ua tiav ntau tshaj 20,000 qhov qauv, uas suav nrog ntau hom qauv sib txawv, peb coj kev txawj ntse ntau yam los rau txhua qhov project.
●Kev Tswj Xyuas ZooPeb siv cov ntsiab lus tswj hwm tseem ceeb thoob plaws txhua theem, txij li kev npaj cov qauv mus rau kev npaj tsev qiv ntawv, kev txheeb xyuas cov ntaub ntawv thiab kev siv bioinformatics. Peb qhov kev saib xyuas zoo ua kom ntseeg tau tias muaj cov txiaj ntsig zoo tas li.
●Cov Tsev Qiv Ntawv RNA Ntau Ntxiv:Peb siv rRNA depletion es tsis txhob linear RNA depletion hauv peb qhov kev npaj ua ntej-tsev qiv ntawv, kom ntseeg tau tias cov ntaub ntawv sequencing tsis yog tsuas yog circRNA tab sis kuj tseem muaj mRNA thiab lncRNA, ua kom muaj kev tshuaj xyuas ua ke ntawm cov ntaub ntawv no.
●Kev Tshuaj Xyuas Xaiv Tau ntawm Kev Sib Tw Endogenous RNA (ceRNA) Networks: Muab kev nkag siab tob dua txog cov txheej txheem tswj hwm cellular.
● Kev Pab Tom Qab Muag KhoomPeb nkag siab tias qhov tseem ceeb ntawm kev tuaj koom, yog vim li ntawd peb txoj kev cog lus txuas ntxiv mus dhau qhov ua tiav qhov project nrog 3 lub hlis kev pabcuam tom qab muag. Thaum lub sijhawm no, peb muab kev saib xyuas qhov project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.
Cov Qauv Yuav Tsum Tau Ua thiab Kev Xa Khoom
| Tsev qiv ntawv | Platform | Cov ntaub ntawv pom zoo | Kev Tswj Xyuas Cov Ntaub Ntawv |
| rRNA depleted directional library | Illumina PE150 | 16-20 Gb | Q30≥85% |
Cov Qauv Yuav Tsum Tau Ua:
Cov nucleotides:
| Conc.(ng/μl) | Tus nqi (μg) | Kev dawb huv | Kev ncaj ncees |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Muaj tsawg los yog tsis muaj protein lossis DNA uas pom ntawm gel. | RIN≥6.0; 5.0≥28S / 18S≥1.0; txwv los yog tsis muaj qhov siab pib |
● Cov Nroj Tsuag:
Cag, Qia los yog Paj: 450 mg
Nplooj los yog noob: 300 mg
Txiv hmab txiv ntoo: 1.2 g
● Tsiaj:
Lub plawv los yog hnyuv: 450 mg
Cov hnyuv lossis lub hlwb: 240 mg
Cov leeg: 600 mg
Pob txha, Plaub hau los yog Tawv Nqaij: 1.5g
● Cov kab mob Arthropod:
Kab: 9g
Cov ntses me me: 450 mg
● Cov ntshav tag nrho:2 lub raj
● Cov Cell: 106 cov hlwb
● Cov ntshav thiab cov ntshav hauv cov ntshav: 6 ml
Kev Xa Qauv Pom Zoo
Lub thawv: 2 ml centrifuge tube (Tsis pom zoo kom siv ntawv ci)
Piv txwv kev sau npe: Pawg + theej piv txwv li A1, A2, A3; B1, B2, B3.
Kev Xa Khoom:
1. Dej khov qhuav: Cov qauv yuav tsum tau ntim rau hauv hnab thiab faus rau hauv dej khov qhuav.
2. Cov raj RNAstable: Cov qauv RNA tuaj yeem ziab hauv cov raj RNA stabilization (piv txwv li, RNAstable®) thiab xa mus rau qhov kub thiab txias hauv chav.
Kev Ua Haujlwm Pabcuam
Tsim qauv kev sim
Kev xa khoom qauv
Kev rho tawm RNA
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Kev tshawb fawb txog bioinformatics
- Kev Tswj Xyuas Cov Ntaub Ntawv
- Kev sib phim ntawm genome
- Kev txheeb xyuas CircRNA
- Kev qhia tawm CircRNA
- Kev tshuaj xyuas qhov sib txawv ntawm kev qhia tawm
kev kwv yees circRNA: kev faib tawm ntawm chromosomal
Cov circRNAs Qhia Sib Txawv - Daim duab qhia txog roob hluav taws
Kev Qhia Txog CircRNAs Sib Txawv - Kev Sib Koom Tes Hauv Hierarchical
Kev txhim kho kev ua haujlwm ntawm circRNA cov noob caj noob ces
Tshawb nrhiav kev nce qib ntawm kev tshawb fawb uas tau pab txhawb los ntawm BMKGene' circRNA sequencing cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm.
Wang, X. et al. (2021) 'CPSF4 tswj kev tsim circRNA thiab microRNA mediated gene silencing hauv hepatocellular carcinoma',Oncogene2021 40:25, 40(25), nplooj ntawv 4338–4351. doi: 10.1038/s41388-021-01867-6.
Xia, K. et al. (2023) 'X oo-responsive transcriptome qhia txog lub luag haujlwm ntawm lub voj voog RNA133 hauv kev tiv thaiv kab mob los ntawm kev tswj hwm kev qhia tawm ntawm OsARAB hauv mov',Kev Tshawb Fawb Txog Phytopathology, 5(1), nplooj ntawv 1–14. doi: 10.1186/S42483-023-00188-8/FIGURES/6.
Y, H. et al. (2023) 'CPSF3 hloov kho qhov sib npaug ntawm cov ntawv sau voj voog thiab kab ncaj hauv hepatocellular carcinoma'. doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al. (2023) 'Kev ntsuam xyuas tag nrho ntawm circRNAs hauv cirrhotic cardiomyopathy ua ntej thiab tom qab hloov siab',Kev Tshuaj Tiv Thaiv Kab Mob Thoob Ntiaj TebIb., 114, p. 109495. doi: 10.1016/J.INTIMP.2022.109495.
