mRNA-seq (NGS) nrog rau cov genome siv
RNA-seq yog ib qho cuab yeej txheem thoob plaws kev tshawb fawb txog lub neej thiab qoob loo, txuas qhov sib txawv ntawm cov genomes thiab proteomes. Nws lub zog nyob hauv kev tshawb pom cov ntawv sau tshiab thiab ntsuas lawv cov lus qhia hauv ib qho kev sim. Nws siv dav rau kev tshawb fawb sib piv transcriptomic, qhia txog cov noob caj noob ces cuam tshuam nrog ntau yam cwj pwm lossis phenotypes, zoo li sib piv cov mutants rau cov tsiaj qus lossis qhia cov lus qhia gene hauv qab cov xwm txheej tshwj xeeb. BMKCloud mRNA (Reference) APP koom ua ke kev ntsuas kev qhia tawm, kev tshuaj xyuas kev qhia tawm sib txawv (DEG), thiab kev tshuaj xyuas cov qauv sib lawv liag rau hauv mRNA-seq (NGS) bioinformatics pipeline thiab sib xyaw cov zog ntawm cov software zoo sib xws, ua kom yooj yim thiab yooj yim rau cov neeg siv. Cov neeg siv tuaj yeem upload lawv cov ntaub ntawv RNA-seq rau huab, qhov twg App muab cov kev daws teeb meem bioinformatic ib zaug xwb. Tsis tas li ntawd, nws muab qhov tseem ceeb rau cov neeg siv khoom, muab cov haujlwm tus kheej uas haum rau cov neeg siv cov kev xav tau tshwj xeeb. Cov neeg siv tuaj yeem teeb tsa cov kev cai thiab xa cov dej num ntawm lawv tus kheej, tshawb xyuas daim ntawv qhia sib tham, saib cov ntaub ntawv / daim duab thiab ua tiav cov ntaub ntawv tshawb nrhiav, xws li: kev xaiv cov noob caj noob ces, kev sib sau ua ke, kev kos duab, thiab lwm yam.
Platform:Illumina, MGI
Txoj Kev Npaj:RNA-Seq
Kev teeb tsa: Pared, huv-cov ntaub ntawv.
Hom tsev qiv ntawv:fr-unstranded, fr-firststrand lossis fr-secondstrand
Nyeem ntev:150 bp
Hom ntaub ntawv:*.fastq, *.fq, *.fastq.gz lossis *.fq.gz. Lub kaw lus yuavcia li txuas cov ntaub ntawv .fastq raws li lawv cov npe ntaub ntawv,piv txwv li *_1.fastq ua ke nrog *._2.fastq.
Tus naj npawb ntawm cov qauv:Tsis muaj kev txwv rau tus lejntawm cov qauv kuaj, tab sis lub sijhawm tshuaj xyuas yuav nce ntxiv raws li tus lej ntawmcov qauv loj hlob.
Cov Ntaub Ntawv Pom Zoo:6G ib qho qauv
