Metatranscriptome Sequencing
Kev Pabcuam Nta
● rRNA depletion ua raws li kev taw qhia mRNA tsev qiv ntawv npaj.
● Sequencing ntawm Illumina NovaSeq.
Kev Pabcuam Zoo
●Kawm Cov Kev Hloov Ntawm Cov Zej Zog Microbial Complex:Qhov no tshwm sim ntawm qib transcriptional thiab tshawb nrhiav cov noob muaj peev xwm tshiab.
●Piav Microbial Community Interactions nrog tus tswv tsev lossis ib puag ncig.
●Comprehensive Bioinformatic Analysis: Qhov no muab kev nkag siab ntawm zej zog taxonomic thiab kev ua haujlwm sib xyaw ua ke, nrog rau kev txheeb xyuas cov noob sib txawv.
●Cov lus piav qhia dav dav ntawm Gene:Siv cov ntaub ntawv tshiab ntawm cov noob muaj nuj nqi rau cov ntaub ntawv gene qhia cov ntaub ntawv ntawm cov zej zog microbial.
●Kev them nyiaj yug tom qab muag:Peb qhov kev cog lus txuas ntxiv dhau qhov kev ua tiav nrog rau 3 lub hlis tom qab kev muag khoom. Nyob rau lub sijhawm no, peb muab cov phiaj xwm taug qab, kev pab daws teeb meem, thiab Q&A ntu los daws cov lus nug ntsig txog cov txiaj ntsig.
Service Specifications
| Sequence platform | Sequence Strategy | Cov ntaub ntawv pom zoo | Data Quality Control |
| Illumina NovaSeq | PEB 150 | 12gb ib | Q30≥85% |
Cov Qauv Xav Tau
| Concentration (ng / μL) | Tag nrho cov nyiaj (µg) | Ntim (µL) | OB 260/280 | OB 260/230 | RIN |
| ≥50 | ≥1.0 | ≥ 20 | 1.8-2.0 Nws | 1.0-2.5 hli | ≥6.5 |
Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Xws li cov kev txheeb xyuas hauv qab no:
● Sequencing Data Quality Control
● Cov ntawv sau ua ke
● Taxonomic Annotation thiab ntau
● Cov lus piav qhia ua haujlwm thiab kev nplua nuj
● Kev nthuav qhia kom muaj nuj nqis thiab kev txheeb xyuas qhov sib txawv
Taxonomic faib ntawm txhua tus qauv:
Beta diversity tsom xam: UPGMA
Functional annotation – GO abundance
Differential taxonomy abundance - LEFSE
Tshawb nrhiav cov kev nce qib uas tau pab los ntawm BMKGene's meta transcriptomics sequencing cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm.
Lu, Z. et al. (2023) 'Acid tolerance ntawm lactate-siv cov kab mob ntawm qhov kev txiav txim Bacteroidales pab tiv thaiv ruminal acidosis hauv tshis yoog raws li kev noj zaub mov zoo',Tsiaj noj, 14, pp. 130–140. doi: 10.1016/J.ANINU.2023.05.006.
Song, Z. et al. (2017) 'Unraveling core functional microbiota nyob rau hauv cov tsoos solid-state fermentation los ntawm high-throughput amplicons thiab metatranscriptomics sequencing',Frontiers hauv Microbiology, 8 (JUL). doi: 10.3389/FMICB.2017.01294/FULL.
Wang, W. W. et al. (2022) 'Novel Mycoviruses Tshawb Pom los ntawm Kev Tshawb Fawb Metatranscriptomics ntawm Phytopathogenic Alternaria Fungus',Kab mob, 14(11), p. 2552. doi: 10.3390/V14112552/S1.
Wei, J. et al. (2022) 'Parallel metatranscriptome tsom xam qhia kev degradation ntawm cov nroj tsuag theem nrab metabolites los ntawm kab thiab lawv lub plab symbionts',Molecular Ecology, 31(15), pp. 3999–4016. doi: 10.1111/MEC.16557.
