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Cov khoom

Metatranscriptome Sequencing

Metatranscriptome Sequencing Featured Duab
  • Metatranscriptome Sequencing

Metatranscriptome sequencing indentifies gene qhia ntawm microbes (ob leeg eukaryotes thiab prokaryotes) nyob rau hauv tej yam ntuj tso ib puag ncig (xws li av, dej, hiav txwv, quav, thiab plab.). ntawm hom, kev ua kom muaj txiaj ntsig zoo ntawm kev qhia cov noob sib txawv, thiab lwm yam.

Platform: Illumina NovaSeq Platform


Cov ntsiab lus kev pabcuam

Cov Qauv Xav Tau

Hom qauv: Tag Nrho RNA;

Qhov hnyav: 2.5 ug;

Cov ntsiab lus: 50ng / ul;

Kev Pabcuam Tswv Yim

Lub tsev qiv ntawv hom: 250-300bp ntxig cDNA lub tsev qiv ntawv;

Sequencing Platform thiab Strategy: illumina NovaSeq Platform; khub-kawg 150bp;

Cov ntaub ntawv pom zoo: 12G raw cov ntaub ntawv / qauv

Kev Pabcuam Ua Haujlwm Flow

coj mus kuaj

Qauv xa tuaj

Kev npaj tsev qiv ntawv

Kev tsim kho tsev qiv ntawv

Sequencing

Sequencing

Cov ntaub ntawv tsom xam

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Kev pab cuam tom qab muag

  • Yav dhau los:
  • Tom ntej:

    • tau ib nqe lus

    Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Khoompawg

    Xa koj cov lus rau peb:

    Ntaus nkag mus tshawb lossis ESC kom kaw