Txo Cov Neeg Sawv Cev Bisulfite Sequencing (RRBS)
Service Specifications
Platform: NovaSeq platform, PE150
Cov tsev qiv ntawv hom: 200-400bp ntxig bisulfite kho DNA tsev qiv ntawv
Sequencing lub tswv yim: Paired-End 150 bp
Pom zoo cov ntaub ntawv tso zis: 10 Gb raw cov ntaub ntawv / qauv
Cov Qauv Xav Tau
DNA ntau: ≥ 2g
DNA concentration: ≥ 20ng / μl.
Purity: OD260/280 = 1.8 txog 2.0 yam tsis muaj degradation lossis RNA paug
Bioinformatics tsom xam
Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
a.Reference genome alignment statistics
Statistics ntawm daim ntawv qhia nyeem
Statistics ntawm sequencing qhov tob thiab kev pab them nqi ntawm C site
Accumulated kev pab them nqi
Digestion efficiency ntawm txhua tus qauv
b.Methylation site nrhiav kom tau
Daim ntawv teev cov qib methylation ntawm C site
BS hloov dua siab tshiab
Methylated cytosine
Methylation theem faib
c.Methylation daim ntawv qhia
Genome dav
Genome ntsiab
d.Kev sib piv ntawm qib methylation ntawm cov qauv
Pairwise Correlation ntawm CG methylation
PCA-CG methylation
CLustering ntawm CG methylation
d.Sib txawv methylation tsom xam
Statistics ntawm DMR
DMR ntev kev faib tawm
Hnoj map
DMR kev faib tawm hauv cheeb tsam tsom
