Txo Cov Neeg Sawv Cev Bisulfite Sequencing (RRBS)
Txo cov sawv cev bisulfite sequencing (RRBS) tso siab rau kev txhawb nqa ntawm CpG cov kob-nplua nuj cheeb tsam los ntawm MspI cleavage ua raws li kev xaiv loj ntawm 200-500/600 bps fragments. Yog li ntawd, tsuas yog thaj tsam ze rau CpG Islands tuaj, thaum cov Islands tuaj CpG nyob deb tsis suav nrog kev tshuaj xyuas. Cov txheej txheem no, ua ke nrog bisulfite sequencing, tso cai rau cov kev daws teeb meem siab ntawm DNA methylation, thiab kev sib txuas ua ke, PE150, tsom tshwj xeeb rau qhov kawg ntawm cov ntawv ntxig es tsis yog nruab nrab, ua kom cov txiaj ntsig ntawm methylation profileing.
Cov txheej txheem no tau tshwm sim los ua tus nqi tsim nyog thiab muaj txiaj ntsig zoo rau Whole Genome Bisulfite Sequencing (WGBS) hauv DNA methylation kev tshawb fawb. Thaum WGBS muab kev nkag siab zoo los ntawm kev tshuaj xyuas tag nrho cov genome ntawm ib qho kev daws teeb meem, nws cov nqi siab tuaj yeem yog qhov txwv tsis pub, uas RRBS tawm tswv yim txo qhov kev sib tw no los ntawm kev xaiv txheeb xyuas ib feem ntawm cov genome. Cov txheej txheem no yog ib qho cuab yeej tseem ceeb uas ua rau muaj txiaj ntsig DNA methylation tshawb fawb thiab txhawb kev paub txog cov txheej txheem epigenetic.
Kev Pabcuam Nta
● Sequencing ntawm Illumina NovaSeq.
● Yuav tsum muaj kev siv genome.
● Lambda DNA yog siv los soj ntsuam bisulfite conversion efficiency.
● MspI digestion efficiency kuj raug saib xyuas.
● Muab ob npaug rau enzyme zom rau cov qauv cog.
Kev Pabcuam Zoo
●Nqi-zoo thiab Efficient Alternative rau WGBS: Nws tso cai rau kev soj ntsuam kom ua tiav ntawm tus nqi qis thiab nrog cov qauv qis dua.
●Ua kom tiav Platform:Peb muab ib qho kev pabcuam zoo tshaj plaws los ntawm kev ua qauv, kev tsim kho tsev qiv ntawv, thiab ua raws li kev txheeb xyuas bioinformatics.
●Kev txawj ntse: Peb muaj kev vam meej thoob plaws ntau hom kev sib txawv. BMKGENE coj ntau tshaj kaum xyoo ntawm kev paub, pab pawg neeg soj ntsuam cov neeg txawj ntse, cov ntsiab lus dav, thiab kev txhawb nqa zoo tom qab muag.
Service Specifications
| Lub tsev qiv ntawv | Sequence Strategy | Pom zoo cov ntaub ntawv tso zis | Kev tswj kom zoo |
| MspI zom thiab Bisulfite kho cov tsev qiv ntawv | Illumina PE 150 | 8gb ib | Q30 ≥ 85% Kev hloov pauv bisulfite> 99% MspI txiav efficiency> 95% |
Cov Qauv Xav Tau
| Concentration (ng / μL) | Tag nrho cov nyiaj (µg) |
| |
| Genomic DNA | ≥ 30 | ≥ 1 | Tsawg degradation los yog paug |
Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
BI tsom xam suav nrog:
● Raw sequencing zoo tswj;
● Daim ntawv qhia txog kev siv genome;
● Kev txheeb xyuas ntawm 5mC methylated bases thiab motif qhia;
● Kev tshuaj xyuas ntawm kev faib methylation thiab kev sib piv piv txwv;
● Kev soj ntsuam ntawm Cov Cheeb Tsam Sib Txawv Methylated (DMRs);
● Cov lus piav qhia ua haujlwm ntawm cov noob muaj feem rau DMRs.
Kev tswj kom zoo: digestion efficiency (hauv genome mapping)
Kev tswj xyuas zoo: kev hloov pauv bisulfite (hauv cov ntaub ntawv methylation extraction)
Methylation daim ntawv qhia: 5mC methylation genome-wide faib
Kev sib piv piv txwv: Tus Thawj Saib Xyuas Kev Tshawb Fawb
Differentially Methylated Regions (DMRs) tsom xam: heatmap
Tshawb nrhiav cov kev tshawb fawb nce qib los ntawm BMKGene tag nrho cov kev pabcuam genome bisulfite sequencing los ntawm kev sau cov ntawv tshaj tawm.
Li, Z. et al. (2022) 'High-fidelity reprogramming rau hauv Leydig-zoo li hlwb los ntawm CRISPR ua kom thiab paracrine yam',PNAS Nexus, 1(4). doi: 10.1093/PNASNEXUS/PGAC179.
Tian, H. et al. (2023) 'Genome-wide DNA methylation tsom xam ntawm lub cev muaj pes tsawg leeg hauv Suav monozygotic ntxaib',European Journal of Clinical Investigation, 53(11), p. ib 14055. doi: 10.1111/ECI.14055.
Wu, Y. et al. (2022) 'DNA methylation thiab duav-rau-hip piv: kev tshawb fawb epigenome-wide koom haum hauv Suav monozygotic ntxaib',Phau ntawv Journal of Endocrinological Investigation, 45(12), pp. 2365–2376. doi: 10.1007/S40618-022-01878-4 ib.
