Iimveliso

IMetabolomics

I-metabolome yi-terminal downstream imveliso ye-genome kwaye iqulethe i-complement epheleleyo yazo zonke i-molecular-weight-weight molecules (i-metabolites) kwiseli, izicubu, okanye i-organism.I-Metabolomics ijolise ukulinganisa ububanzi obubanzi beeamolekyu ezincinci kumxholo we-physiological stimuli okanye izifo zesifo.Iindlela zeMetabolomics ziwela kumaqela amabini ahlukeneyo: i-metabolomics engajoliswanga, uhlahlelo olubanzi olujoliswe kulo lonke uhlalutyi olunokulinganiswa kwisampulu kubandakanywa iikhemikhali ezingaziwayo zisebenzisa i-GC-MS/LC-MS, kunye ne-metabolomics ekujoliswe kuyo, ukulinganiswa kwamaqela achazwe ngokwemichiza kunye i-biochemically annotated metabolites.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

IiMfuno zeSampulu

Iimetabolomics ezingajoliswangaIintlobo zeesampuli		Isampulu ecetyiswayoisixa	Uphindaphindo lwebhayoloji
LI-C-MS/GC-MSi-metabolomics engajoliswanga	Izicubu	200mg	Isityalo ≥6; Isilwanyana ≥10; Umntu ≥30;
	Iplasma/serum	200ul
	Imixholo yeFecal/Intestinal	150mg
	Iiseli	1*10⁶
	umchamo	500ul
	Ulwelo lweRumen	1ml
Imetabolomics ekujoliswe kuyoIintlobo zeesampuli		Isixa sesampulu esicetyiswayo	Uphindaphindo lwebhayoloji
I-Phytohormone/Tryptophane/I-Energy Metabolism/ lipids oxidized/Carotenoid;carotinoid	Izicubu	500mg	Isityalo ≥3; Isilwanyana ≥6;
	Iplasma/serum	500ul
	I-Fecal	1000mg
	Iiseli	1*10⁷

IiNgcaciso zeNkonzo

Imetabolomics engajoliswanga:
1.Itekhnoloji eqolileyo kunye nezinzile, isisombululo esiphezulu kunye nokukhetha okulungileyo
2. Gqibezela uvimba weenkcukacha;
3. Ukulungele uhlalutyo lweematriki eziyinkimbinkimbi, ukubonelela ngolwazi olubanzi kuhlalutyo olunye;

Imetabolomics ekujoliswe kuyo:
1.Sebenzisa isiqinisekiso somgangatho wangaphakathi we-isotopu ukuphucula umgangatho kunye nokuchaneka kobungakanani bezinto;
2.Inkqubo yolawulo lomgangatho ukuqinisekisa ukuthembeka kwedatha.